A Clinical And Laboratory Researches Of Antibacterial Synergism Of Xianglian Square

Objective:(1)To observe the antibacterial synergism of Xianglian suppository with Miconazole suppository in the treatment of the pure VVC clinically. At the same time to observe The characteristic of pure VVC.(2)To observe the antibacterial synergism of Xianglian Lotion、huanglian ecoction solution with nitric acid Miconazole solution in the laboratory(3)To further improve the standardization research of Xianglian Series square preparations in the topical treatment of refractory mucocutaneous fungal disease.(4)To Preliminarily study the advantages of combining traditional Chinese and Western medicine treatment of VVC.Methods:Clinical research:According to the principle of randomized single blind,65patients were divided into test group and control group who were included accordding to the strict standards with the.35patients of test group and30patients of is35and control group. The test group was given the Xianglian suppository and miconazole suppository, The control group was treated with miconazole suppository, A total of7days for the treatment. Observed the basic conditions of all patients, When the patient came to see thedoctor for the first time. The review time is the following:three days after the end of treatment, after the first menstrual clean, after the secede menstrual clean. Recorded the score before and behind the treatment. According to the main effect, therapeutic index, to evaluate the curative effect. Compared with the efficiency of the chi-square test.laboratory procedure:According to the1997CLSI (American clinical and Laboratory Standards Institute) broth microdilution antifungal susceptibility testing of yeasts reference methods promulgated the "(M27 program)", Using the broth microdilution method, The MIC of theXianglian lotion、nitric acid Miconazole solution、huanglian lotion、xianglian lotion with nitric acid miconazole solution、huanglian lotion with nitric acid miconazole effecting on Candida albicans and Candida glabrata were determined. Combined antibacterial index effect were used to analysis of the application of drugs; Using the independent samples t test, to analysis of the MIC of nitric acid Miconazole solution、xianglian lotion、huanglian lotion.Results:Clinical study results:For the65patients, the age is the smallest17years old, the biggest51years old, mainly concentrated in the20-40years old this stage. The27patients with unmarried sexual life(41.54%),38patients with married, most of were married and childbearing age. In the diet,16cases of patients with eating spicy,6cases of eating cold,3cases of eating sweets; In the education,11cases of junior high school,30cases of college,22cases of undergraduate,2cases of master. Menstruation is normal; There were no obvious tobacco and alcohol addiction; pressure for general pulse, tongue coating showed a red tongue greasy yellowish, dialectic is damp, wet weight in the heat.A week after treatment, observation of curative effect,3days after the end of treatment, the test group was57.1%, the effective rate was60%in the control group, no significant difference in efficacy between the two groups; after the first menstrual clean, the test group was57.1%:the effective rate of control group53.3%, there were no statistically significant differences effect of the two; after thesecond menstrual clean, the test group was57.1%, the control group was53.3%, there was no statistically significant difference in efficacy between the two.To observe the efficacy index, evaluating scores before and after the treatment. After the three days of treatment, the test group was (60%), the control group was23.33%, two groups were the effective treatment, but there were significant differences between the experimental group effect, curative effect is better than that of the control group.The results of laboratory research:For the effect on Candida albicans:nitric acid miconazole solution MIC value was:14. Oug/ml; Xianglian lotion MIC value was13.54mg/ml; Huanglian lotion MIC value was13.65mg/ml; We can said that for the inhibition of Candida albicans in vitro MIC value, Xianglian lotion13.54mg/ml, a considerable effect of Coptis Decoction13.65mg/ml and nitric acid miconazole solution14. Oug/ml. Huanglian lotion is more sensitive than Xianglian lotion on Candida albicans.Nitric acid Miconazole solution with Xianglian lotion effect on Candida albicans, two combined antibacterial index1<FICI=1.25≤4, The Nitric acid Miconazole solution and Xianglian lotion combined effect on Candida albicans, both of them were unrelated effect.Nitric acid Miconazole solution and huanglian lotion on Candida albicans, combined inhibition index of the two is0.5<FICI=0.53≤1, that of miconazole nitrate and huanglian lotion combined with effect on Candida albicans two additive effect.The effect on Candida glabrata:Nitric acid Miconazole solution MIC value was:14.88ug/ml, the average MIC of Xianglian lotion:8.40mg/ml, Huanglian lotion;MIC means:9.38mg/ml; can be said for the in vitro inhibition of Candida glabrata MIC value, Nitric acid Miconazole solution14.88ug/ml, quite effect of Coptis Decoction9.38mg/ml and miconazole nitrate14.88ug/ml. No differences in sensitivity to unilateral Coptis Decoction and Fufang Xianglian lotion of Candida glabrata.Nitric acid Miconazole solution with Xianglian lotion on Candida glabrata, two combined antibacterial index1<FICI=2.33≤4, that of Nitric acid Miconazole solution and Xianglian lotion combined effect on Candida glabrata, two for unrelated effect.Nitric acid Miconazole solution and huanglian lotion on Candida glabrata, combined inhibition index of the two is FICI=0.70≤0.5, that of miconazole nitrate and Coptis decoction combined with effects on Candida glabrata two synergistic action.The effect on Candida albicans and Candida glabrata, Nitric acid Miconazole solution, huanglian lotion were no significant difference. But for the effect on Candida albicans and Candida glabrata, The Xianglian lotion is more sensitive to Candida glabrata.Conclusion:Clinical results show that:In the treatment of simple vulvovaginal candidiasis, Xianglian suppository combined miconazole suppository can obviously improve the clinical symptoms, and provide clinical efficiency.Laboratory results show that:Xianglian lotion, huanglian lotion and Nitric acid Miconazole solution effect on Candida albicans, Candida glabrata have bacteriostasis. For Candida albicans, huanglian lotion is more sensitive than Xianglian lotion; Nitric acid Miconazole solution in Xianglian lotion both acting on Candida albicans and Candida glabrata, were unrelated effect; miconazole nitrate and Coptis Decoction on Candida albicans showed an additive effect, effect on Candida glabrata were synergistic.