| Objective:Five viscera holding spirit is an important theory on the relationship between zangfu and mind in traditional Chinese medicine. The study from the basis of theoretical analysis of the clinical literature, the point of view of animal experiments will explore the relationship between the spleen and stomach and consciousness. To explain the mechanism of action between the stomach and the mind has important theoretical significance and clinical value.Methods:Combing stomach as the theoretical basis of the five internal organs, the center of the whole, to explore the impact of stomach conscious activity under physiological and pathological states. Consciousness abnormal to today’s society, a high incidence of depression, for example, to collect the clinical literature of acupuncture in the treatment of depression in the commonly used medical databases, statistics select meridians and acupoints data and analysis. In the present study all rats were singly housed and chronic unpredictable stress was adopted to establish the depression model of rats. Applying electro acupuncture to Shao yin Heart Channel of Hand and Stomach Channel of Foot Yang ming, compared with flextime in the western medicine group, the therapeutic effects of electro acupuncture (EA) at the heart meridian and stomach meridian acupoints on depression were assessed. The present study investigates the mechanism of electro acupuncture(EA) at the heart meridian and stomach meridian acupoints on depression at the level of hippocampus by adopting open-field behaviors to test behavioral indexes of rats, observing cell arrangement and its morphological change of hippocampus neurons with hematoxylin-eosin staining method (HE staining), using western blot method to measure CREB expression levels in hippocampus, detecting CREB content in hippocampus with the method of Immunohistochemical method.Results:Five elements to the earth for the statue, which spleen is so important just because for the earth attachment. The ancients advocating bit of neutral position the center performance out of the phenomenon of metaphases cereal essence towards the maintenance of the body, that is an important source of qi and blood and respected human stomach. The whole body of the gas lift access even for a moment to turn pivot inseparable from the spleen and stomach, the stomach is actually an air-lift the pivot. Physiological stomach nourishes the five internal organs, to reconcile the power to help the five internal organs. Pathology, a sick stomach can lead directly to the five internal organs disharmony; then led to the five internal organs against the chaos of the overall air-yin and yang of the gas phase is not smooth; meridians blood runs disorders can also be caused by whole body gas-poor, internal organs and no security, and overall co-ordination uncoordinated thus affecting conscious activities. From clinical medical record analysis the stomach adjust the five internal organs, the overall impact of the mechanism of conscious activity, whether the stomach with the Shiyi, stomach presided over the five internal organs, the overall gas dryer disorders, or stomach metabolism of blood and body fluids, spleen, stomach and economic impact, pathogenesis key from not open blood, yin and yang. Reflect the results of the clinical literature, the frequency is much higher than the treatment of depression select yang Yin and depression in disease resistance is yin diseases also show that depression is not a separate dirty organ of the disease. Prompt diagnosis and treatment of depression should grasp the nature of the blood against the chaos, under the guidance of the overall concept for the treatment of air-conditioning blood, turn pivotally key Note "recuperates yang channel, attaches great importance to the spleen" treatment ideas.The Zusanli group of EA, Shenmen group depression rat level of activity and vertical activity increase in the number of obvious. Fluoxetine hydrochloride group, electro-acupuncture Zusanli group, EA Shenmen group on sugar consumption experiments compared with the model group, sucrose consumption was significantly increased. EA Zusanli group, EA Shenmen between the two groups, there was no significant statistical differences. Changes in body weight, it is found that the relative ease of electro-acupuncture Zusanli points Shenmen group weight of depression in rats than other groups. The HE staining observed in rat hippocampal neurons structure, model group rat hippocampal CA3neurons rehearsal disorder. EA Shenmen group, electro-acupuncture Zusanli group, and fluoxetine hydrochloride group hippocampal CA3neuronal degeneration significantly reduced cell bodies arranged in order, clearer cellular level structure is clearer. But EA Shenmen group, electro-acupuncture Zusanli group cells compared with fluoxetine hydrochloride group situation a little better, there is no significant differences. Immunohistochemical method observed the expression of CREB, model group was significantly lower than the other groups, EA Shenmen group was significantly higher than in group Zusanli; rat hippocampal CA3model of depression in rat hippocampal CREB expression was determined by Western Blot, model group The expression levels of CREB the lowest electricity Zusanli group than Shenmen group. Regulatory role in hippocampal CA3area of electro-acupuncture Shenmen enhanced CREB expression stronger than the EA enough Zusanli view from the entire hippocampus CREB expression, electro-acupuncture Zusanli better than EA Shenmen.Conclusions:1. Five zang-organs hidden spirit theory, the spleen and stomach is the center of the five zang-organs as a whole, is the key to the five zang-organs overall the spirit.2. Stomach regulating mechanism of the overall impact of the conscious activity of the five internal organs can not be separated from the blood, yin and yang.3.Depression belongs to consciousness disorders belong to yin disease, disease resistance, and also show that depression is not alone dirty organs of the disease. Prompt diagnosis and treatment of depression should grasp the nature of the blood against the chaos, under the guidance of the overall concept for the treatment of air-conditioning blood, turn pivotally key Note "recuperates yang channel, attaches great importance to the spleen" treatment ideas.4. Cupping the meridian of yang ming foot zusanli and hand less Yin the heart sutra cave shenmen can improve pathological state in rats depression, have the function of the prevention and treatment of depression prompts to Canon of five zang-organs theory and heart hidden spirit theory as the basis, guiding treatment curative effect is abnormal mind5. Cupping zusanli and shenmen adjusting mechanism of pathogenesis of depression is associated with an increased content of hippocampus after CREB, speculated that after CREB levels increase, the protection functions of nerve to strengthen the curative zusanli of the hippocampus after CREB levels increase shenmen is stronger than the influence of electric acupuncture point to consider the reason may be that acupuncture, the meridian of yangming foot directly adjust the upper abdomen spleen and stomach, generate adjustable factors after operation the qi and blood of the mind activity process mechanism may be associated with the changes of nerve-endocrine-immune network, the gut brain axis has great possibility to be involved in a series of reactions... |
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