Er Gene Polymorphism And Month After Syndromes Was Less And The Curative Effect Of Correlation Research

Hypomenorrhea:themenstrual cycleis normal,duration andamount of menses are reduced. It may develop amenorrhea or premature ovarian failure if the hypomenorrhea does not treated in time,and it may lead to Habitual abortion or infertility.So it is important to treat hypomenorrhea in time.Objective1Based on frequency analysis and cluster analysis,study the distribution law of TCM syndromes of the hypomenorrhea.Further to provide a statistical basis for the hypomenorrhea of TCM syndromes.2The present study was undertaken to find out the variations in the estrogen receptor-a gene polymorphisms in susceptibility to hypomenorrhea with polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP).To find out the relation to risk of the hypomenorrhea of TCM syndromes and the estrogen receptor-a gene PvuⅡ (rs2234693) and Xbal (rs9340799) and TA repeat ((TA)n). Further to provide the molecular biology basis for the hypomenorrhea of TCM syndromes.3Thepresent case-controlstudywas examinedthevariations of the endometrial thickness and endometrial morphology and endometrial blood flow in hypomenorrhea by Transvaginal color Doppler ultrasound. Examined the associations between them and the estrogen receptor-a gene PvuII and Xbal and (TA)n.4To find out the efficacy of Chinese Traditional Medicine over the hypomenorrhea. Analysisthe variationsof theendometrial thicknessandendometrial morphologyand endometrialbloodflow after treatment.Studythe associationsbetween the efficacyof Chinese Traditional Medicine and the estrogen receptor gene PvuⅡ and XbaⅠ and (TA)n.Methods1The presentstudy based on the questionnairesabouttheTCM syndromes of hypomenorrhea from159cases. Studythedistribution law of TCM syndromesof the hypomenorrhea with frequency analysis and cluster analysis.2The present case-control study was undertaken to find out the variations in the estrogen receptor-agene polymorphisms in susceptibility to hypomenorrhea with polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP).To find out the relation to risk of the hypomenorrhea of TCM syndromes and the estrogen receptor-α gene PvuⅡ and Xbal and (TA)n. 3The present case-control study was examined the variations of the endometrial thickness and endometrial morphology and endometrial blood flow in hypomenorrhea by Transvaginal color Doppler ultrasound. Examined the associations between they and the estrogen receptor-α gene PvuⅡ and Xbal and (TA)n.4To find out the efficacy of Chinese Traditional Medicine over the hypomenorrhea. Analysis the variations of the endometrial thickness and endometrial morphology and endometrial blood flow after treatment. Study the associations between the efficacy of Chinese Traditional Medicine and the estrogen receptor-gene PvuⅡ and Xbal and (TA)n.Results1The cluster analysis showed the distribution characteristics of TCM syndromes of hypomenorrhea from159case were follows:the kidney deficiency and liver stagnation syndrome>the kidney deficiency and blood stasis syndrome>the Qi and blood deficiency syndrome>the liver kidney deficiency syndrome>the kidney deficiency and phlegm dampness syndrome.2The Analysis of frequency showed the distribution laws of TCM syndromes of hypomenorrhea from159case were follows:the most symptoms of premenstrual irritability, premenstrual breast pain, blood black-red and with clots, lassitude, soreness and weakness of waist and knees.3The differences of genotype frequencies of ESR1rs2234693PP/Pp/pp genotype carriers between hypomenorrhea patients and general population were statistically significant (P<0.05). The ESR1rs9340799XX/Xx/xx genotype carriers were statistically significant too (P<0.05). The genotype frequencies of ESR1were as follows:PP<Pp<pp, XX<Xx<xx; The differences of allele frequencies of ESR1rs2234693P/p genotype carriers between hypomenorrhea patients and general population were statistically significant (P<0.05). The ESR1rs9340799X/x allele carriers were statistically significant too (P<0.05). The risk of P allele cause hypomenorrhea measured as odds ratio (OR) equal to0.55times to p allele.The risk of X allele cause hypomenorrhea measured as odds ratio (OR) equal to0.483times to x allele.4The differences of single nucleotide polymorphism frequencies of ESR1were statistically significant (P<0.05) between hypomenorrhea patients and general population. The study showed that the single nucleotide polymorphism frequencies of ESR1ppxx were more susceptibility to hypomenorrhea.5The differences of genotype frequencies of ESR1rs2234693PP/Pp/pp genotype carriers were statistically significant (P<0.05) among the different TCM syndromes of hypomenorrhea. The ESR1rs9340799XX/Xx/xx genotype carriers were statistically significant too (P<0.05). The study showed that the genotype frequencies of ESRl pp and xx genotype were as follows: the kidney deficiency and phlegm dampness syndrome>the kidney deficiency and liver stagnation syndrome>the kidney deliciency and blood stasis syndrome>the liver kidney deficiency syndrome>the Qi and blood deficiency syndrome. The differences were statistically significant(P <0.01). The differences of allele frequencies of ESRl P/p and X/x genotype carriers were statistically significant (P<0.05) among the different TCM syndromes of hypomenorrhea. The study showed that the genotype frequencies of ESRl P allele were as follows: the Qi and blood deficiency syndrome>the liver kidney deficiency syndrome>the kidney deficiency and blood stasis syndrome>the kidney deficiency and liver stagnation syndrome>the kidney deficiency and phlegm dampness syndrome. The study showed that the genotype frequencies of ESRl X allele were as follows: the Qi and blood deficiency syndrome>the liver kidney deficiency syndrome> the kidney deficiency and blood stasis syndrome> the kidney deficiency and liver stagnation syndrome=thekidney deficiency and phlegm dampness syndrome.6The differences of single nucleotide polymorphism frequencies of ESRl carriers were statistically significant (P<0.01) among the different TCM syndromes of hypomenorrhea. The study showed that the single nucleotide polymorphism frequencies of ESRlwere as follows: the kidney deficiency and phlegm dampness syndrome>thc kidney deficiency and liver stagnation syndrome>the kidney deficiency and blood stasis syndrome> the liver kidney deficiency syndrome>the Qi and blood deficiency syndrome.The differences were statistically significant(P<0.01).7Thedifferencesof theestrogen receptor-α TA repeat((TA)n)werestatistically significant(P<0.01) between hypomenorrhea patients and general population. The study showed that the ESRl (TA)19were more susceptibility to hypomenorrhea. the differences werestatistically significant(P<0.05). The risk of ESR1(TA)19cause hypomenorrhea measured as odds ratio (OR) equal to6.176.The differences of ESRl(TA)n carriers were statistically significant (P<0.01) among the different TCM syndromes of hypomenorrhea. The study showed that the ESRl (TA)19were as follows: the Qi and blood deficiency syndrome>the liver kidney deficiencysyndrome thekidney deficiency andblood stasis syndrome>the kidney deficiency and liver stagnation syndrome>the kidney deficiency and phlegm dampness syndrome. The differences were statistically significant(P<0.01).8The study showed that the variations of the endometrial thickness and endometrial morphology and endomelrial blood flow in hypomenorrhea patients and general population were statistically significant (P<0.01).But the differences about they and theestrogen receptor-α gene PvuⅡ and XbaⅠ and (TA)n were non-significant. 9The efficacy of hypomenorrhea therapy with Chinese Traditional Medicine were was achieved in72.5%, in100%of patients with Hypochondrium pain, in90%of patients with premenstrual breast pain, in88.89%of patients with the lower abdomen pain,and in80%of patients with soreness and weakness of waist and knees.Under Chinese Traditional Medicine therapy improving of endometrial blood flow was achieved in65%,The differences were statistically significant.Conclusion1The study showed the distribution characteristics of TCM syndromes of hypomenorrhea were follows:the kidney deficiency and liver stagnation syndrome> kidney deficiency and blood stasis syndrome>Qi and blood deficiency syndrome> liver kidney deficiency syndrome> kidney deficiency and phlegm dampness syndrome.2The distribution laws of TCM syndromes of hypomenorrhea were follows:the most symptoms of premenstrual irritability, premenstrual breast pain, blood black-red and with clots, lassitude, soreness and weakness of waist and knees.3The results of the present study suggest that the ESR1rs2234693and rs9340799gene are associated with hypomenorrhea. Patients with low frequencies of the p allele and x allele were more likely to impede the hypomenorrhea.The study showed that the single nucleotide polymorphism frequencies of ESR1ppxx and ESR1pp genotype and xx genotype were more susceptibility to hypomenorrhea.4The results of the present study suggest that the ESR1rs2234693and rs9340799gene are associated with the different TCM syndromes of hypomenorrhea.Patients with high frequencies of the p allele and x allele were more likely to respond to the hypomenorrhea with the excessive and the deficiency syndrome. especially kidney deficiency and blood stasis syndrome and the kidney deficiency and liver stagnation syndrome. The single nucleotide polymorphism frequencies of ESR1ppxx were more susceptibility to the hypomenorrhea with the excessive and the deficiency syndrome.5The results of the present study suggest that the estrogen receptor-α TA repeat ((TA)n) are associated with the hypomenorrhea.the ESR1(TA)19were more susceptibility to hypomenorrhea.6The results of the present study suggest that the variations of the endometrial thickness and endometrial morphology and endometrial blood flow are associated with the hypomenorrhea, but no significant correlation with the different TCM syndromes of hypomenorrhea.7The efficacy of hypomenorrhea therapy with Chinese Traditional Medicine were was achieved in72.5%, it may obviously improve the endometrial blood flow.