Herba Leonuri Skin Photoaging Of Experimental Research

Objective:The experiment is according as the basic theory of TCM, to investigate LSH’s influence on the activity of SOD, GSH-Px and CAT in the skin of photoaging mice, wich result from ultraviolet irradiation, as well as the content of HA, MDA and HYP in their skin, the expression levels of Bcl-2protein、Bax and TGF-β1; and to investigate LSH’s effect on the structure of the photoaging mice. To establish the animal model of skin photoaging as well as to study the protective effects of LSH.Methods:Healthy KM mice were divided into11groups randomly and respective-ly,45days age, weight18+2.2g, female. Falls into negative control group (A), external-model group(B), oral-model group(C), positive druggery defending group(D), external low-LHS-dosage preventing group(E), external medium-LHS-dosage preventing group(F), external high-LHS-dosage preventing group(G), oral low-LHS-dosage preventing group(H), oral medium-LHS-dosage preventing group (I), oral high-LHS-dosage preventing group (J), external medium-LHS-dosage preventing group(afer UV irradiation)(K). The mice in all groups but group A were irradiated to simulate skin photoaging by long-term ultraviolet irradiation.30minutes before UV irradiation, external0.9%physilological saline,0.4ml to B Group;Oral0.9%physiological saline,20ml/kg to C group;external VitE to D Group;At the same time, the mice those in E, F, and G groups were given LHS externally and which in H, I, J groups through mouth. Staining skin with HE, under40times light microscope, the structure of dermal was observed. While the changes of the content of H2O2, MDA, HYP, as well as the activity of SOD, GSH-Px and CAT, the changes of the expression levels of Bax, Bcl-2and TGF-β1in the skin were measured by biochemical test method in each group of the experiment.Results:1. In model group, the activity of SOD, CAT and GSH-Px in dermal were decreased(p <0.01), while the contents of the MDA, H2O2were increased seriously (P<0.01). on the contrary, the content of HYP was reduced (P<0.01). The skin biopsies appear photoaging look.2. Compared with the model group, the contents of HYP in external and oral groups were made a great progress (P<0.01, P<0.05); It can make differences with the contents of HYP in low-LHS-dosage external and oral groups (P<0.05);midium-LHS-dosage external and oral groups(P<0.05). It can make any differences with the contents of HYP in low-LHS-dosage external and high-LHS-dosage oral groups (P>0.05).3. Compared with the model group, in external and oral groups, the contents of MDA were decreased, the SOD’s energy were elevation (P<0.01). The contents of MDA in external and oral groups were made a great progress (P<0.01);It can make any differences with the contents of MDA in medium-LHS-dosage external and oral groups (P>0.05). It can make differences with the contents of MDA in high-LHS-dosage external and oral groups (P<0.05);The SOD’s energy were make any sense both in the external and oral three groups(P>0.05).4.Compared with the model group, the activity of CAT’s, GSH-Px’s energy were elevation, the contents of HA were decreased (P<0.05, P<0.01). The CAT’s energy were make any sense both in the external and oral three groups(P>0.05). The contents of HA were make any sense both in the medium, high-external and oral groups (P>0.05).5. Compared with the model group, the expression of Bax was down-regulation, Bcl-2was up-regulation, TGF-β1was up-regulation(P<0.01). The expression of Bax was make any sense both in the external and oral three groups (P>0.05). The expression of Bcl-2was make sense between the high-dosage external and oral groups(P<0.01). The expression of TGF-β1was make sense between the high-dosage external and oral groups (P<0.01).6. Compared with the model group (B), the energy of SOD, CAT, GSH-Px were increased (P <0.01);The contents of HA, MDA were decreased (P<0.01);the contents of HYP was increased(P<0.01);The expression of Bcl-2was up-regulation, Bax was down-regulation, TGF-β1was up-regulation (P<0.01).Conclusions:1. Long-term ultraviolet irradiation can make skin of mice become photoaging. UV-irradiation induced photoaging of mice, the derma tissue of mice have oxidative damage obviously, the cells apoptosis were accelerate.2. LHS can improve the pathological film of mice who have photoaging damage. It has discrepancy in three external groups, It has no-discrepancy in three oral groups (P>0.05). The external groups have therapy effect than oral-groups.3. LHS could eliminate free radicals itself, and by increasing the ability of inhibiting the oxidative damages of the organism,, remit the trend of cell apoptosis to prevent photoaging damage.4. LHS can also repair the photoaging damage of the mice derma tissue.