| Objective:Atrial fibrillation (AF) was one of the most common arrhythmia diseases threateninghuman health. The aim of the present study was to investigate the current prevalence ofatrial fibrillation in Xinjiang adult population, to explore the distribution of geneticpolymorphisms of KCNE1in healthy Han and Uighur population and assess theassociation between the genetic polymorphisms of KCNE1and atrial fibrillation inUighur and Han population in Xinjiang.Methods:By means of four-stage selected random sampling, adults aged over35year oldwere used to analyze the prevalence and relative risk factors in different nationality, ageand sex.The sampled adult population were collected26locality from23municipalitiesand7locality and5autonomous county in Xinjiang. The proportion of male to femaleaccounted for50%each.409Uighur and406Han healthy persons were detected thegenotypes of the rs1892593(SNP1), rs1805127(SNP2) of KCNE1gene by the use ofpolymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)analysis and directly sequence methods. Analysis the sequence with other population. In afine designed pair matched hospital based case-control study, we selected303patientswith atrial fibrillation and328control in Uighur population,307patients with atrialfibrillation and330control in Han population. They were detected the genotypes of thers1892593(SNP1), rs1805127(SNP2) of KCNE1gene by the use of polymerase chainreaction-restriction fragment length polymorphism (PCR-RFLP) analysis and directlysequence methods. Results:(1) A total of14618adults were surveyed. The prevalence of AF was0.40%, whichwould be0.35%after being standardized.The prevalence of AF was0.31%,0.25%and0.59%in Han, Uygur and Kazak population respectively after being standardized. Theprevalence of AF was0.53%in men and0.28%in women, men had higher prevalencethan women (P＜0.05). The prevalence of Af had a increased trend with age by agedistribution,75years and over age groups reached3.5%, significantly higher than otherage groups (P＜0.01). Among all the AF cases, valvular, nonvalvular, alone AF were31%,36.2%and32.8%respectively. Among all the AF cases, paroxysmal and chronicatrial fibrillation were19%,81%respectively. The prevalence rate of stroke in patientswith Af group was15.5%and3.7%in non-AF group, Af group had higher prevalencethan non-AF group (P＜0.01). The ratios of patients who receive antiarrhythmic therapywere51.7%, βblocker, digitalis and Amiodarone were22.6%,20.7%and8.6%respectively. The ratios of patients who receive antithrombotic therapy were29.3%,aspirin was the main drug about25.9%, only8.6%of patients often taken and96.6%ofpeople never taken warfarin. According to the multi-factor logistic analysis, the or valuebetween AF and age, heart valvular disease, chronic heart failure was1.09,1.90and10.65respectively.(2) The frequencies of AA, AG and GG genotypes of SNP1were25%,49%and26%in Uighur group while ones were36%,47%and17%in Han group; the frequencies of Aand G alleles were50%and50%in Uighur group while ones were59%and41%in Hangroup. There were significant difference in distribution of genotypes and alleles betweenthese two groups (both P＜0.01). The frequencies of AA, AG and GG genotypes of SNP2were13%,46%and41%in Uighur group while ones were21%,45%and34%in Hangroup; the frequencies of A and G alleles were36%and64%in Uygur group while oneswere44%and56%in Han group. There were significant difference in distribution ofgenotypes and alleles between these two groups (both P＜0.01). As to Uighur population,there were significant difference of SNP1in distribution of genotypes and allelescompared with European, Han in Beijin, Japan and Sub-Saharan African population (bothP＜0.05). As to Han population, there were significant difference of SNP1in distributionof genotypes and alleles compared with European and Sub-Saharan African population(both P＜0.05), neither genotypes nor alleles of SNP1were significantly differentcompared with Han in Beijin and Japan population (both P＞0.05). As to Uighurpopulation, there were significant difference of SNP2in distribution of genotypes and alleles compared with Sub-Saharan African population (both P＜0.01), neither genotypesnor alleles of SNP2were significantly different compared with European, Han in Beijinand Japan population (both P＞0.05). As to Han population, there were significantdifference of SNP2in distribution of genotypes and alleles compared with Japan andSub-Saharan African population (both P＜0.05), and there were significant difference ofSNP2in distribution of genotypes compared with European population (P＜0.05);neither genotypes nor alleles of SNP2were significantly different compared with Han inBeijin (P＞0.05), and there were no significant difference of SNP2in distribution ofalleles compared with European (P＞0.05).(3) Both in Uighur and Han population, there were significant difference of SNP2genotypes and alleles between AF group and control group (both P＜0.01), the frequencyof G allele was more common in the AF group than in the controls (both P＜0.01).Otherwise, neither genotypes nor alleles of SNP1were significantly differentbetween these two groups. SNP1is of linkage disequilibrium with SNP2both in Uighurand Han population of two groups.In the3haplotypes constructed with SNP1and SNP2of Uighur, the A-G haplotype was more frequent in the AF group than in the controls (P＜0.01), but the frequencie of A-A haplotype were lower in the AF group than in thecontrol group (P＜0.01). In the3haplotypes constructed with SNP1and SNP2of Han,the A-G haplotype was more frequent in the AF group than in the controls (P＜0.01), butthe frequencie of A-A and G-A haplotypeds were lower in the AF group than in thecontrol group respectively (both P＜0.01). According to the multi-factor logistic analysisin Uighur, the OR value between AF and LAD, AG, GG genotypes of SNP2was1.09,1.80and1.72respectively. According to the multi-factor logistic analysis in Han, the ORvalue between AF and LAD, AG, GG genotypes of SNP2was1.06,1.66and2.03respectively.Conclusions:(1) The total adults population prevalence of Af in Xinjiang was similar to reports atabroad and a bit low to reports at home, the age, gender epidemiological characteristicswere similar to reports at home and abroad and the classification characteristics weredifference from reports at home and abroad.There were no difference of prevalence of AFin Han, Uygur and Kazak. The treatment of atrial fibrillation in Xinjiang was not ideal,should strengthen the control of AF. Age, heart valvular disease and chronic heart failuremay be risk factors of Af in Xinjiang. (2) The polymorphism of1892593and rs1805127of KCNE1were significantdifference between Uighur and Han population of Xinjiang. As to Uighur population,there were significant difference of SNP1in distribution of genotypes and allelescompared with European, Han in Beijin, Japan and Sub-Saharan African population,. Asto Han population, there were significant difference of SNP1in distribution of genotypesand alleles compared with European and Sub-Saharan African population. As to Uighurpopulation, there were significant difference of SNP2in distribution of genotypes andalleles compared with Sub-Saharan African population. As to Han population, there weresignificant difference of SNP2in distribution of genotypes and alleles compared withJapan and Sub-Saharan African population, and there were significant difference of SNP2in distribution of genotypes compared with European population.(3) The polymorphisms of rs1805127of KCNE1was associated with the risk of AFin Uighur and Han population of Xinjiang. A-G haplotype of may be an genetic markerand A-A haplotype may be an protective factor of AF in Uighur and Han population, G-Ahaplotype may be an protective factor of AF in Han population. LAD and AG, GGgenotypes of rs1805127maybe more increase the AF risk in Uighur and Han populationof Xinjiang. |
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