The Experimental Research For The Influence Of Wenshen Fang On Subclinical Hypothyroidism Rats’ Vascular Injury

Objective:to observe the effect of Wen Shenfang healing the pathological changes of SCH model rats and its blood vessels’da-mage;Method:select8healthy Wistar rats who are6months old from the whole50rats as the control group; Then make the operation that cut the thyroid off all from the rested rats and inject L-T4to these oprated rats to make sure these rats being with the SCH status. When find all these rats had become into SCH status, separate these rats into4groups—model、TCM (Mediate)、TCM(high)、L-T4group. Then turn drugs into these rats’stomachs for30days.Then1.Use the chemiluminescence trial to detect the level of the function of all these rats ’Thyroid-FT3, FT4, TSH.2. Use Toshiba DBA-120type fully automatic biochemistry analyzer to detect the blood fat’s concentration of the experimental rats.3. Detectthe rats’ liver tissues ’HMG-CoA reductase and serum MCP-1, ET-1level by Elisa method.4. Remove the rat the main thoracic aorta ’s2cm, then homogenate them, take the clear liquid, extract the total RNA, using the RT-PCR technology to expand gene fragments in vitro, amplifying product through the electrophoresis, through to the electrophoresis photos on grey value for testing, compare the LOX-1mRNA’s relative content.5. Take rat body materials from them after irrigation treatment stomach, remove the rat the thoracic specimens, prepare patho-logical specimens, through the light and electron microscopic for observating experimental rats’ thoracic aorta ’s microstructure and ultrastructure; Results:1. The group compared with normal, model rats, serum TSH level increased significantly (P<0.01). Group compared with model, Chinese traditional medicine group TSH level is reduced significantly (p<0.05); TSH leels significantly reduce western medicine group (P<0.05); High dose group of traditional Chinese medicine TSH leels significantly reduce (p<0.05). FT3between groups, FT4level no difference obvious (P>0.05) model group liver TT3content, group compared with normal, have reduced significantly (P<0.05); High in the group, Chinese traditional medicine group, western medicine group TT3content are liver is obviously improve the model group (P<0.05), and especially the Chinese traditional medicine the high group most obvious increase;2. High dose group of traditional Chinese medicine, compared with controls, plasma TC, TG, LDL-C, hdl-c level no obvious difference (p>0.05);, compared with Chinese traditional medicine group, serum lipid four level no obvious difference (P>0.05); Group compared with western medicine, there was no significant difference (P>0.05); And model group, the comparative plasma TC, TG, LDL-C level reduced significantly (P<0.05), serum levels of HDL-C level obviously improve (P<0.05). Model group, compared with controls, plasma TC, TG, LDL-C level increased significantly (P<0.05), and HDL-C level reduced significantly (P<0.05);3. The experimental results through the statistics processing, it was found that the high dose group of traditional Chinese medicine, compared with controls, the liver HMG-CR, and serum MCP-1, ET-1level no obvious difference (p>0.05);, compared with Chinese traditional medicine group, the liver HMG-CR, and serum MCP-1, ET-1level no obvious difference (P>0.05); Group compared with western medicine, but no obvious difference (P>0.05); And model group, the comparative liver HMG-CR, and serum MCP-1, ET-1level reduced significantly, and have obvious difference (P<0.05).4. Of blank control, high dose group of traditional Chinese medicine, western medicine group, Chinese traditional medicine group, the model group rats blood vessels of LOX-1mRNA RT-PCR products after electrophoresis. Through the central region of the scan the grey value analysis, then, will the corresponding group respectively their LOX-1the mRNA products and electrophoresis own groups of beta-Actin mRNA of the electrophoresis of grey value product comparison, between the ratio of numerical calculation. Results show that, compared with the normal control group, the model group LOX-1the relative content of the mRNA rose substantially (P<0.05); And the model group of LOX-1the relative content is the mRNA, high dose group of traditional Chinese medicine, western medicine group, the Chinese traditional medicine group LOX-1the relative content of decreased significantly (P<0.05), and especially in high doses of Chinese traditional medicine group dropped the most obvious;5. Microscopic structure is mainly manifested in the artery lining losing smoothy state, endothelial cells’ damaging, arrangement, losing the normal order of things, the wide gap between endothelial cells, monocyte macrophages infiltrates, and the formation of the foam cells, smooth muscle cells deformation, the hyperplasia; In the ul trastructural level is behaved for endothelial cell injury, structured exception, the film lost smooth level off state in cells, mitochondria swell, rough endoplasmic reticulum reduce, cytoplasm show clear thin, endothelial gap expand, visible mononuclear macrophages, an infiltration performance; Through the Chinese medicine dose after intervention, rats the degree of vascular lesions can be obviously lighter, and with the increase of temperature renal party dose, the clinical JiaJian rats vascular lesions in the case of the apparent ease, and compared with western medicine in the treatment group, also can embody the corrective and clinical JiaJian rats vascular lesions of the slightly advantage. Conclusion:Taking "all thyroid cutness+daily subcutaneous injection L-T40.95ug/100g"-the modeling methods for the preparation of success-can succeed in making up SCH model rats. Using the method of preparation of SCH model rats, peripheral organization in the organization of the state JiaJian low T3, and liver tissue of the HMG-CoA reductase content on the high side, because the liver high HMG-CoA reductase level and low T3state SCH organization in rat models of high TC, LDL-C especially high OX-LDL level of blood lipid disorders, which happen to the fat of toxicity of vascular endothelial function, or for endothelial cell direct damage; And this kind of damage ET-1, MCP-1etc, in turn, aggravating vascular lesions, form relatively serious vascular lesions; The warm renal party intervention SCH rats and find that temperature renal party will really improve experimental rats SCH of state and increase the utilization rate of the week outside organization T3, adjust blood lipid disorders, especially reduce OX-LDL level and rel ieve for the damage and the vascular endothel ial directly caused by the secondary vascular lesions, the curative effect is obvious, and compared with western medicine group, it can improve endothelial injury, reduce vascular lesions.Chinese traditional medicine group especially high dose group of traditional Chinese medicine effect is more outstanding. This is for the wide use of temperature in the clinical renal party to provide evidence of the animal experiment and the foundation. Title:The experimental research for the influence of Wenshen Fang on subclinical hypothyroidism rats ’vascular injury Profession:Internal medicne of TCM Name:Pan Liwen Tutor:Professor Chen Ruquan...