| Objective:To observe the symptoms, digestion and absorption function, transport capacity of membrane protein, mitochondrial structure and function of of intestinal epithelium in spleen-deficiency rats; explore the relationship between invigorating spleen effect of moxibustion and each link of intestinal epithelium energy metabolism; elucidate molecular biological mechanism of invigorating spleen effect of moxibustion; provide experimental basis for moxibustion invigorating spleen theory.Methods:The study was divided into three parts. Part one:40 healthy SD rats were randomly divided into 4 groups, namely, blank group (Group A), model group (Group B), moxibustion group (Group C) and herbs group (Group D),10 rats in each group. The model was established by intragastric administration with Chinese Rhubarb infusion. The appetite, weight, tractive tail resists time and spleen-deficiency symptoms integral were recorded. The intestinal propulsive rate and content of D-xylose were detected. Part two:groups and processing method are same as part one. The content of ATP, activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase in jejunal epithelium were detected by colorimetric method. The expression of GLUT-1 in jejuna epithelium was measured by immunohistochemistry. Part three:groups and processing method are same as part one. The mitochondrial ultrastructure of jejuna epithelium was observed by electron microscope. The content of SDH and CCO of jejuna epithelium was detected by ELISA method. The expression of COX I of jejuna epithelium was detect by western-blot method.1. Compared with group A, the increasing rate of weight and appetite, tractive tail resists time in group B were significantly decreased (P<0.01), while symptoms integral increased (P<0.01). Compared with group B, the increasing rate of weight and appetite, tractive tail resists time in group C and D were significantly increased (P<0.01), while symptoms integral decreased (P<0.01). There were no significant difference between group C and D.2. Compared with group A, the intestinal propulsive rate and content of D-xylose in group B were significantly decreased (P<0.05 or P<0.01). Compared with group B, The intestinal propulsive rate and content of D-xylose in group C and D were significantly increased (P<0.05 or P<0.01). There were no significant difference between group C and D.3. Compared with group A, the content of ATP, activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase in jejunal epithelium in group B were significantly decreased (P<0.05 or P<0.01). Compared with group B, the content of ATP, activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase in jejunal epithelium in group C were significantly increased (P<0.05 or P<0.01). There were no significant difference between group C and D. The expression of GLUT-1 in jejuna epithelium in group C was higher than that in group B (P<0.01).4. The mitochondrial ultrastructure of jejuna epithelium in group B was destroyed seriously, while that in group C and D was destroyed mildly. Compared with group A, The content of SDH and CCO of jejuna epithelium in jejunal epithelium in group B were significantly decreased (P<0.05 or P,0.01). Compared with group B, The content of SDH and CCO of jejuna epithelium in jejunal epithelium in group C and D were significantly increased (P<0.05 or P<0.01). There were no significant difference between group C and D. The expression of COX I in jejuna epithelium in group C was higher than that in group B (P<0.01), but there were no significant difference between group B and D.1. Moxibustion could decrease symptom integral, increase weight, appetite and physical strength, which suggested moxibustion could invigorate spleen.2. Moxibustion could increase the intestinal propulsive rate and content of D-xylose, which suggested that moxibustion could improve digestion and absorption function of spleen-deficiency rats. 3. Moxibustion could increase the content of ATP, activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase, and expression of GLUT-1 in jejuna epithelium, which suggested moxibustion could promote ATP production and improve content of transport capacity of membrane protein.4. Moxibustion could promote mitochondrial ultrastructure repair of jejuna epithelium, increase content of SDH and CCO, upregulate expression of COX I, which suggeste moxibustion could improve the structure and function of mitochondria repairment to enhance metabolism.5. "Spleen", mitochondria and metabolism are. Moxibustion invigorating spleen effect might be related to repairing improve the structure and function of mitochondria repairmen, improve activity of mitochondrial enzyme, enhance metabolism to improve transport function of intestinal epithelium and movement-absorbtion function.6. Moxibustion and Sijunzi decoction both had invigorating spleen effect, but the mechanism might be different. |
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