Screening And Identification Of Hair Follicle Development Related MicroRNA In Cashmere Goat

Inner Mongolia cashmere goat has excellent producing wool traits,is precious germplasm genetic resources,so research the cashmere goat fluff development mechanism,explore the important candidate genes associated with villi is particularly important. High-throughput sequencing technology is applied in this experiment,this study collected 10 cashmere goat in different periods of fetal skin, according to previous research results.to cashmere goat fetus skin hair follicle initiation, growth and development of three key period,55 to 45 days and 65 days down producing goat fetal body side of the skin (the same period of the three samples as biology repeat) RNA samples, after mixing amount to transcriptome sequencing,and the results are of bioinformatics analysis and qRT-PCR verification. Test results show that:1. The use of high-throughput sequencing method for fetal skin hair cashmere goat at different time microRNAs and mRNA expression data.Oar-let-7 and oar-miR-200 family of micrornas in pregnancy samples of 55 days and 65 days of pregnancy,45 rise significantly relative to pregnancy, suggests that it may be a key in the development of follicles of micrornas.2.The application of bioinformatics analysis forecast oar-let-7 and oar-miR-200 target genes for GLDC, GPM6A, LRP.3.Test using PCR technology to cashmere goat hair follicles in the sequencing results validated the key mirna, results show that the quantitative results express trend with the sequencing results are basically identical.4.Test the qRT-PCR technology for cashmere goat hair follicles sequencing results verified. Results show that the predicted the target genes,respectively is GLDC, GPM6A and LRP in cashmere goat fetal skin hair follicle development in higher quantity.