Male Sterile And Lutein Inheritance&Screening Molecular Marker In Tagetes Erecta L.

In order to obtain new varieties of Tagetes erecta L. with intellectual property and highornamental values, selective male sterile line of Tagetes erecta L.,and seach for high luteincontent character geneticdevelopment.The main achievements are as follows:1. Experiment with208A male sterility plant and201B、205B、207B、217B ferility plantfor parent, record performance of the Fertility and Sterility in F₂generation separation ratio is3:1.Then back cross with F₁,the separation ratio is1:1. The Experimental Data Show thatMale sterility of Tagetes erecta L. was1of recessive allele nuclear genes controlled.2. Base on improve the petal color economic characters of male sterile line, use darkcolour208A×217B、208A×201B F₂backcross with W217B、 W201B,then sistercross.Selective male sterile line of Tagetes erecta L.3. Through the morphology, cytology observation, AB217male sterile line is structuraltype male sterile, Speculation it is base on B gene afunction.4. The measure SOD,POD, MDA, Fpro, soluble sugar content inAB217male sterileline concluded that W217male sterile is due to SOD,POD lively and MDA, Fpro, Solublesugar content descend. For speed up breeding that measure SOD,POD,in leaf and bud,measure POD and Soluble sugar content in flower to distinguish between fertile plants andheterozygous plant.5.Through217A（jacinth）×203B（white） P₁, P₂, F₁, BC₁^p1F₁, BC₂^p2,F₁,F₂nvestigateion,the type of jacinth flower colors heredity and lutein content heredity is2pair of additivedominance epistasis major gene+additive-dominance-epistasis polygenes. The relativitybetween flower colors heredity and lutein content heredity is tightly.6.Found147SSR in Tagetes erecta and Tagetes patula EST. Dinucleotide is the most,62.29%. TC to be the most abundant in dinucleotide. TTG to be the most abundant intrinucleotide.1pentanucleotide, and tetranucleotide not be found. Design28SSR primer withPrimer premier5.0software. The SSR analysis identified a total of17bands among the217A×203B F₂, with4bands being polymorphic. From the results of amplification,4from8SSR primers showed no different bands between217A×203B F₂.