Studies On The Vitrification And Prevention In The Culture Of Pigment Marigold

In this experiment, High efficiency in vitro regeneration system of pigment marigold was developed using potted seedings and seedings from seeds were use as explants.in subculcure process,and the vitrification in subculcure have been studied.The aim is of establish high efficiency in vitro regeneration system of pigment marigold which was to reduce vitrification rate and raise propagation rate.Results were as follows:1.The establishmeng of the regenerating system in pigment marigoldAxillary buds as explants to compare the different hormone levels on axillary bud induction, proliferation, rooting, The results showed that:in the bud induction in, MS+6-BA0.5mg/L+ NAAO.lmg/L pigment marigold buds in explants of the best bud induction medium; Proliferation in the bud, to MS+6-BA1mg/L+NAAO.Olmg/L is appropriate, value-added factor of 11.5 is the test the best pigment medium subculture marigold; The most suitable rooting medium for the pigment of marigold 1/2MS+IBA0.1mg/L.2.The influencing factors and preventive2.1 The effects of the hormones on the vitrificationThe results showed that:6-BA marigold vitro proliferation of pigment and vitrification of the rate coefficient significantly affected. When 6-BA concentration of 0.1mg/L with the 0.5mg/L were significantly different between the vitrification of the lowest, Either below or above this concentration, vitrification of the increase in varying degrees,0.1mg/L of 6-BA on the vitrification affect the pigment of marigold plantlets occurs most significant, and when 6-BA concentration of 0.lmg/L when the proliferation of pigment marigold highest coefficient,3.56.2.2The effects of the sucrose,agar,AC on the vitrificationThe results showed that:sucrose concentration on pigment marigold Subculture has some influence on the pigment marigold, the sucrose concentration of 30-50g/L when the pigment is to reduce the glass marigold plantlets appropriate concentration. Agar concentration of pigment marigold Subculture have a certain influence, agar concentration of 1.2% is the optimum concentration affect the glass. Impact on the multiplication coefficient, the agar concentration of 0.8% of the maximum proliferation factor for the appropriate concentration. Carbon concentration from 0 to 0.1%, the lowest level of pigment marigold glass, but carbon concentration of 0.3%, the pigment marigold vitro multiplication coefficient up to 2.46.2.3The effects of the other additives on the vitrificationThe results showed that:polyvinyl alcohol concentration of 1.8g/L, the lowest rate of pigment marigold glass, the highest proliferation coefficient, was 3.16. Add penicillin to the medium and MET inhibition can play a role in the rate of glass, proliferation factor also improved, but the proliferation of MET can significantly improve the coefficient of 4.54.2.4The preventive measures to overcome vitrification Based on the above findings:The test conclude that the vitrification to prevent the pigment of marigold plantlets comprehensive measures:(a) use 0.1mg/L6-BA, add 0.O1mg/LNAA; (b) the use of sucrose concentration (30-50g/L); (c) using the agar concentration of 1.2%; (d) add the activated carbon concentration of 0.1%; (e) the use of 1.8g/L of polyvinyl alcohol concentration.