The Study Of Clinical Efficacy And Mechanism On Jia Jian Yi Wei Tang Ftreating With Yin-Deficiency Of Spleen And Stomach Pedo-anorexia

Objective:Under the guidance of TCM theory and combined with modem method of scientific research, the study, taking the way of "replenishing Yin and strengthen stomach"as the direction, centerson the effect of Jia Wei Yi Wei Tang on the trial animal's gastrointestinal function,digestion, assimilation and the regulating of its gastrointestinal hormone so as to further approach the pathological mechanism of immature anorexia and the acting mechanism of "replenishing Yin and strengthen stomach" in dealing with immature anorexia by improving the gastrointestinal function and hormone disorder.Methods:Two herbalp reparaitons:Jia Wei Yi Wei Tang and Er Bao Granules are used to conduct i.g treatment on immature anorexic rats which are fed with special forage developed by Nanjing University of T.C.M. Meanwhile, any forage intake and weight changes on the rats are closely watched and recorded.To be specific, using method of radioimmunization to check and measure the amount changes on MLT,N T,CCK and NPY of rats;using improved Anson-Mirshy method to measure the pharmaceutical effects of Jia Wei Yi Wei Tang on rats' activities of gastric protein enzyme and the gastric acid secretion; And using determination of enzyme-linked immunosorbent assay in plasma leptin levels in rats; according to the principle of randomized comparison from the clinical practice respect, investigates curative effect of 30 cases Children with Yin-Deficiency anorexia using Jia Wei Yi Wei Tang.Conclusions:The results show that Jia Wei Yi Wei Tang is effective on inhibiting the gastrica cids ecertion of anorexia rats,and obviously activating the pepsin of anorexic rats; it may also works well on the regulation of trial anorexia rats'MTL and NT disorder. What's more,it decrease anorexia rats'content of CCK in plasma and decrease the expression of CCK-R mRNA in colon smooth muscle cells of the anorexia rats. However, plasma Leptin and NPY levels of anorexia rats have little effect. Accordingly,the weakening of gastrointestinal motility and that of the digestive and assimilative function maybe defined as the important factors which contribute a lot to immature anorexia with Yin Deficiency; in addition, a close connection is found between the incerasing of NT along with the deficiency of MTL and the immature anorexia. The expression of CCK-R mRNA was obviously increased in colon smooth muscle cells of the anorexia pedo-rats, which is one of the mechanisms of anorexia.Modified Yiwei decoction can decrease the expression of CCK-R mRNA in colon smooth muscle cells of the anorexia rats. The clinical study results suggest that the total curative effect of Jia Wei Yi Wei Tang treating Yin-Deficiency anorexia is 93.3%, has no obvious difference. (P>0.05) But the Jia Wei Yi Wei Tang group has superiority on the evident control rate compares to the control group.And it has better effection on long-term improvement in children with height, weight and improving content of Zn elements in blood of children anorexia compares to Er Bao Granules group (P<0.05). It illustrates Jia Wei Yi Wei Tang treating Yin-Deficiency anorexia has unique superiority itself, worth researching a step further.