Effect Of Micropowder Buyang Huanwu Decoction On Neurologic Deficits And Nogo-A Of MCAo Model Rat

Objectives:To observe the effect of Micropowder Buyang huanwu Decoction on neurologic deficits,Nogo-A and Nogo-A recepter NgR of MCAo model rat. Discovery the possible mechanism of the improving on neurologic deficits and the characteristic in useing.Methods:MCAo mice models were reproduced by Intraluminal Suture.One hundred and twenty rats survival were classified into three groups after 2 weeks: model group,traditional decoction group and Micropowder-form group(the dosage is 1/3 of traditional decoction group).Rats in each group were classified into four time points:before treatment,2 weeks after treatment,4 weeks after treatment,and 6 weeks after treatment respectively(10 rats for each time point).Forty another normal rats were taken as control group.1.Each rat was scored by neuroethology before treatment,2 weeks after treatment,4 weeks after treatment,and 6 weeks after treatment respectively.Six items were evaluated:Placing test,Beam Balance test,Adhesive Removal test,Beam-traversing test,Limb-use asymmetry test,and Single-pellet reaching test.2.Rats were killed after neuroethology score,and were analyzed the expression of Nogo-A protein and the receptor NgR protein using the Immunochistochemistry method and RT-PC method.Results:1.Model rats showed neurologic deficits after surgery.The neurologic deficits still remained in 6 weeks after surgery.The score of neurologic deficits for model group, traditional decoction group and Micropowder group were statistically different from control group(P＜0.01) before treatment or in 6 weeks after surgery.Compare with 2 week after the surgery,The neurologic deficits showed spontaneous recovery after 6 weeks'treatment.The score of neurologic deficits in 6 weeks after treatment was statistically significant different from the score of which before teatmeant(P＜0.01). Buyang huanwu Decoction can improve the recovery of neurologic deficits.The score of neurologie deficits in traditional decoction group and Micropowder group were different from model group(P＜0.01 or 0.01＜P＜0.05) in 6 weeks after treatment. The difference in the score between Micropowder group and traditional group after 2 weeks'treatment,4 weeks'treatment and 6 weeks' treatment were not significant(P＞0.05).2.The expression of mRNA and protein for Nogo-A could be observed in 2 weeks after surgery.The difference in IOD between the baseline group(8.57±0.79) and the control group(0.61±0.12) was significant(P＜0.01).The difference in RT-PCR result between the baseline group(0.52±0.13) and the control group(0.10±0.02) was significant(P＜0.01).The expression of mRNA and protein for Nogo-A decreased along with survival time increasing.Immunochistochemistry and RT-PCR were found significantly different(P＜0.01) in model group between the time of 2 weeks(8.57±0.79,0.52±0.13) and the time of 6 weeks(5.23±1.22,0.29±0.08). Both form of Chinese medicine can reduce the expression of mRNA and protein for Nogo-A.Compared with model group(5.23±1.22),significant differences(P＜0.01) in IOD were found for traditonal group(1.84±0.38) and Micropowder group (1.88±0.20) in 6 weeks after treatment.Compared with model group(0.29±0.08), significant differences(P＜0.01) in mRNA were found for traditional group(0.14±0.06) and Micropowder group(0.15±0.04) in 6 weeks after treatment.There was not significant differences between 1/3 dosage of Micropowder-form and traditional decoction.The expression of the receptor NgR protein of Nogo-A and the effect on NgR expression of stroke brain tissue by medicine were similar to the result of Nogo-A. Conclusions:1.MCAo rats presented different extent symptoms of neurologic deficits.The symptoms of neurologic deficits were relieved and the neuroethology score were improved after therapy by taking Buyang huanwu Decoction.The difference in the effect between Micropowder-form(the dosage is 1/3 of traditional decoction) and traditional decoction was not significant.2.The expression of Nogo-A protein,the receptor NgR protein and mRNA were increased after surgery in MCAo rats.Buyanghuanwu Decoction can reduce this expression,and there was not significant difference in the effect between Micropowder -form(the dosage is 1/3 of traditional decoction) and traditional decoction.