| Chapter 1 The Study of literature of the facial color-diagnosis of traditional chinese medicalThe facial color-diagnosis originated in "Huang Di Nei Jing". ancient physicians have many expatiations and development. However, it was difficult to find the breakthrough about the study of the facial color-diagnosis.Many of them were only the things on summarizing experiences and the qualitative analysis. To study the principle and the significance of the facial color-diagnosis, to provide the theoretical basis for the quantitative and the objective of the facial color-diagnosis,we must classify and review the previous literature on the basis of a comprehensive and systematic exposition of ancient books.Objective:For the facial color-diagnosis of traditional chinese medical,understanding its history and origin, diagnosis principle and clinical application,discussing the research status at present,the existing problems, and research countermeasures.Methods:By studying the literature about the facial color-diagnosis of traditional Chinese medical, especially studying previous physicians'successful examples in the clinical diagnosis and treatment of diseases, to classify and review them in the principle of diagnosis,clinical application and modernization research,and to clear the research status, the existing problems,and research countermeasures for the facial color-diagnosis.Results:1.The facial color-diagnosis originated in "Huang Di Nei Jing". ancient physicians have many expatiations and development which believed "the Jing gas out of the viscera, the facial color along with the Jing gas bacame more and more fine-looking."2. There were many successful examples of the facial color-diagnosis in clinical application,which can identify causes of disease and disease-card changes and judge the severity of the disease and the fine-ups of the Jing gas.3. modernization research of the facial color-diagnosis, provide some objective and quantitative data for the clinical diagnosis. However, it is difficult to get firm conclusions for the lack of strict scientific design and less duplication of tests.4. Present color-diagnosis devices have drop far behind the science and the technoloy. applying the technology of photoelectric blood stream volume in the facial color-diagnosis of traditional chinese medical, developing the photoelectric blood stream plethysm may be one of the breakthrough objectifying the facial color-diagnosis.Conclusions:1.The facial color-diagnosis of traditional chinese medical has a long history, there were many ancient classic exposition.2. "The Jing gas out of the viscera, the facial color along with the Jing gas bacame more and more fine-looking."is the principle of the facial color-diagnosis of traditional chinese medical.3. On the facial color-diagnosis applications, ancient physicians have many successful examples, for the cause of disease, symptom change, disease severity, and filling or declining of the Jing gas.4. The study objectifying the facial color-diagnosis and the study of the looking form mechanism show that the facial color-diagnosis lack theoretical innovation and interdisciplinary research; how to introduce modern scientific and technological achievements, make the facial color-diagnosis objective and quantitative, and applied it to clinical diagnosis,is one of the urgent problem to be solved in facial color-diagnosis.Chapter 2 Development and Application of The traditional Chinese medical photoelectric blood stream plethysmFor a long time, the facial color-diagnosis research is stagnant,and difficult to achieve a breakthrough. on the one hand, facial color-diagnosis base only on the gathering experence.It is intuitive conduct on the basis of qualitative analysis,lack accurate quantitative indicators. So it is difficult to quantify and repeat. on the other hand,color-diagnosis lack theoretical innovation and interdisciplinary. how to use modern scientific and technological achievements, to make the facial color-diagnosis objective and quantitative,is an important problem that we must study.By using the photoelectric blood stream plethysm technology in traditional Chinese medicine theory of the facial color-diagnosis, developing the photoelectric blood stream plethysm is perhaps one of the breakthrough objectifying facial color-diagnosis.Objective:1.Developing GD-3 photoelectric blood stream plethysm of the facial color-diagnosis.2. determine the indicators of detection and the methods of operation.3. In order to obtain stable waveform parameters, studying factors affecting blood stream plethysm.Methods:By developing and using photoelectric sensors, signal amplifier, matching Pclab biological signal acquisition system,display the waveform on the computer screen, identify the name, the symbols and measuring units of the parameters indicator such as the amplitude,the time, the ratio,the area,the angle and so on,and establishing the methods and steps operating the instrument.At the same time, by comparing the waveforms of the same subject in different circumstances, analyse of the pros and cons of measurement conditions, this provide experimental basis for controling measuring conditions.Results:1.We developed GD-3 photoelectric blood stream plethysm of the facial color-diagnosis. Choosing suitably light-emitting diodes and resistors to make photosensitive photoelectric sensors, by the data acquisition and processing, A /D digital conversion, detection filtering, signal amplification, with Pclab biological signal acquisition system,the graph in real-time is displayed on the computer screen,the parameters results is calculated,and the analytical report is submited.2.According to the waveform of the photoelectric blood stream plethysm displayed on a computer screen, to determine the name, the symbols and measuring units of the parameters indicator such as the amplitude,the time, the ratio,the area,the angle and so on,and establishing the methods and steps operating the instrument.3.Photoelectric blood flow volume is a graph of weak Biological signals being vulnerable to interference such as different physiological and psychological conditions, measuring different parts,different conditions, different testing environment, and so on. We study impact factors to reduce the interference of various factors, to access to higher-quality waveforms. By comparing the waveforms of the same subject in different circumstances, analyse of the pros and cons of measurement conditions, this provide experimental basis for controling measuring conditions. Conclusions:1.Successfully developed GD-3 photoelectric blood stream plethysm of the facial color-diagnosis.2.Determine its waveform detection indicators and the methods of operation.3.By the study controlling the impact factors such as measureing conditions,we can acquire higher quality and waveform stable technical parameters.This provide the experimental basis for objectifying the facial color-diagnosis in the traditional Chinese medicine.Chapter 3 The Clinical Research of photoelectronic blood volume graph in Chinese facial color-diagnosisAfter studying facial color-diagnosis in the literature and developing photoelectric device of facial blood flow volume diagnosis, the reseach of its clinical application is particularly important. How normal color of different parts on a healthy face is? how pathology five color and different colored pathological subtype?how facial color of HBSS?the PBSP parameters of QSBSS and QDBS? the relationship between Facial PBSP parameters and FVB and their formation mechanism? The study has important significance.Objective:1. Finding out characteristics of PBSP parameters for often color and disease color, different positions, different diseases, different card type, different subtypes. 2.Understanding the relation between characteristics of photoelectric blood flow volume parameters and Nailfold microcirculation, tongue microcirculation, Pulse Detection.3.By observing the relation between the amplitude of photoelectronic blood flow volume graph and the content of HbO2, ET/NO, TXB2/6-K-PGF1 a, GMP-140, to grasp photoelectronic blood flow volume graph and the formation mechanism of the facial color-diagnosis.Methods:1.Photoelectronic Blood Volume Measurement, Nailfold microcirculation and tongue microcirculation tests, Pulse Detection, Presses the instrument working instruction operation.2. The content of HbO2, the blood plasma NO,the blood plasma ET,and the content of TXB2,6-K-PGF1α, the GMP-140 is determined with the HICN color method, the nitric acid reductases law, the emission immunity analytic method and the enzyme to unite the immunity adsorption law respectively.Results:1. The values of healthy facial color five regular PBSP were compared: The different values of Hb, Hd, He, Hf, Tw, Hb/Tab, Hd/Hb, He/Hb, Hf/ Hb, Tw/Tag due to different sites. The Hb/Tab assumes the tendency of the nasal tip> left and right cheek> forehead and chin, but the Hd/Hb, He/Hb, Hf/Hb and the Tw/Tag are on the contrary. Healthy face three types of color casted (partial red group, partial white group, partial yellow group) were compared:there are significant differences of Hb/Tab, Hd/Hb, He/Hb, Hf/ Hb and Tw/Tag, Hb/Tab became smaller and Hd/Hb, He/Hb, Hf/Hb, Tw/Tag become bigger with partial facial often red color from white→partial→Pianhuang,2. The values of pathological five color facial PBSP were compared:there were significantly difference of Hb/Tab, He/Hb, Tw/Tag, Tab/Tag [Tae-Tab]/Tag compared with normal group. The comparation results of Pathological colored groups each other as follow:reddish complexion group Hb/Tab and [Tae-Tab]/Tag was significantly higher; whitish complexion group Tab/Tag was significantly higher than that of the group, but its Hb/ Tab lowest; yellowish complexion group He/Hb decreased significantly; bluish complexion group Tw/Tag and He/Hb were higher than those in the group; blackish complexion group [Tae-Tab]/Tag was significantly lower than that of the group. Pathological reddish complexion, red group Hb increased significantly, rubeosis group Tae/Teg increased significantly, blushed group to He/Hb increased significantly for it. Pathological whitish complexion,pale group Hd low, whitish group [Tae-Tab]/Tag minimum, Tab /Tag maximum value, and whiteness group Tab/Tag normal, but Hd significantly higher than the other two group characteristics. Pathological yellowish complexion, etiolate Group Tae extension, yellowish puffy group He /Hb increased jaundice group Hb/Tab increased significantly characterized. Pathological bluish complexion, the lowest bluish-white group Hd, Tw smaller slatey group, cyanochroia Group Tw extended, and Hb increased significantly. Pathological blackish complexion, light black patients [Tae-Tab]/Tag lower,dry black group Tae/Teg increased, while dark group to Tw/Tag for increased significantly.3. The photoelectric blood volume amplitude indices of CHD HBSS group were compared with health group:Hd, He, Hf were reduced. He/Hb, Tw/Tag were significantly increased, and Hd/Hb, [Tae-Tab]/Tag, Hf/Hb is markedly reduced.4.Photoelectric facial blood volume results of deficiency of heart-energy syndrome, deficiency of heart-blood syndrome, cariac blood stasis syndrome and health group showed that:The Hb and Hf of deficiency of heart-energy syndrome were significantly lower than other groups, the Tab and Tae of deficiency of heart-blood syndrome were significantly higher than other groups, while the Hd and Tw of cariac blood stasis syndrome were significantly higher than other groups.The Hb/Tab and Hf/Hb of deficiency of heart-energy Syndrome were lower than deficiency of lung-QI. Deficiency of heart-blood syndrome was compared with deficiency of liver-blood syndrome,Tab/Tag increased significantly, and [Tae-Tab]/Tag decreased significantly. The Hd/Hb and Tw/Tag of cariac blood stasis syndrome were significantly higher than liver blood stasis syndrome.5. The facial PBSP parameters was compared with QSBSS, QDBS, and healthy group:The He/Hb of two Syndrome Group was significantly higher than healthy group, the Hd and Tab/Tag of QSBSS were significantly higher than other groups, the Hb and Hb/Tab of QDBS were significantly lower than other groups.6.The facial PBSP parameters of the PCHD HBSS and PS were compared:HBSS and PS were compared with the normal group, Hb, He, Hf and Hf/Hb decreased significantly;The result of Synchronous detection NFM showed that:HBSS compared with other groups, output branch diameter and the top diameter loop increased significantly, velocity slowed significantly, shape score,flow score and total score were heightened significantly; Synchronous detection TTM found that the HBSS of Papillae in diameter, thickness and velocity on the cortex significantly decreased, and the number of nipple stasis expanded significantly increased in the cortex integral, integral form, flow pattern points total score significantly increase; synchronous detection EPG found:cariac blood stasis syndrome Hf, Hd/Hb, Hf/Hb decreased, and He/Hb increased significantly.7.With PBSP amplitude increased, the number HbO2 content gradually increased, ET values are gradually decreased, NO values are rising, TXB2 were numerical has been gradually declining,6-K-PGF1αvalues are rising, GMP-140 numerical are gradually declining. And low volatility and high volatility group there were significant group differences.PBSP amplitude and HbO2 content with a positive correlation, correlation coefficient of 0.523, p= 0.000; ET and the content of a negative correlation, correlation coefficient of-0.319, p= 0.001; NO content with a positive correlation, correlation coefficient of 0.398, p= 0.000; and TXB2 in the negative correlation, correlation coefficient of-0.339, p= 0.001; and 6-K-PGF1αin a positive correlation, correlation coefficient of 0.304, p= 0.000; and GMP-140 in a negative correlation, correlation coefficient of-0.199, p-value of 0.045.Between HBSS, PS and the normal group, the PBSP amplitude significantly different; But the HbO2 content HBSS group is lowest, PS group next best, the normal group is highest, in both the February 2 was significant difference;the ET content among the three groups had no significant difference; the NO content HBSS group is lower than the normal group, the two have the significance difference; the TXB2 content HBSS group was significantly higher than the PS group and the normal group,6 K-PGF1α.content was significantly lower than the normal group, the two have the significance difference; and GMP-140 content HBSS group was significantly higher than PS group and the normal group, the two have the significance difference.Conclusions:1.Often facial five-volume blood flow parameters demonstrated that the different spot blood stream volume parameter is different. Partial red, Partial white, Partial yellow, the flow volume parameters are different. Tips flow volume parameters change is the formation of color often face physiological basis, reflecting the "looking-blood volume" in the mechanism.2. looking pathological parameters of the blood volume change reflects the characteristics of pathological colored and mechanisms.Mianchi are pathological Hb/Tab [Tae-Tab]/Tag increased significantly; pathology of white, Tab/Tag increased pathological side to Huang He/Hb decreased; pathology of Green, Tw/Tag, He/Hb significantly increased pathology of black, Tw/Tag, He/Hb High, [Tae-Tab]/Tag significantly lower.3. CHD in patients with HBSS photoelectric facial blood flow volume parameters:Hd, he, hf, (Tae-Tab)/Tag, Hd/Hb, Hf/Hb decreased significantly, and He/Hb, Tw/Tag was significantly higher. Tip cardiac dysfunction, vascular reduce flexibility, increased peripheral resistance, reduce compliance coronary artery blood stasis one of the basic pathological changes.4. Heart Disease Syndrome facial blood-flow volume of consultation and tests showed:deficiency of heart-energy syndrome patient by facial color pale primarily, has at the same time chlorosis, Hb and Hf were significantly lower than other group; deficiency of heart-blood syndrome patient by facial color chlorosis primarily, has at the same time pale, Tab and Tae were significantly higher than other group; cariac blood stasis syndrome patient by facial color schungite primarily, has at the same time dark, Hd and Tw were significantly higher than other group.5.QSBSS and QDBS facial blood flow parameters showed He/Hb increased significantly. Hd and Tab/Tag of QSBSS significantly increased; Hb and Hb/Tab of QDBS significantly reduced.6.Synchronous detection through PCHD facial blood flow volume map,NFM, TTM and EPG discover:PBSP parameters with three, the target assists mutually, clinical supplementary,to study heart, blood and veins in the physiological and pathological features and peripheral micro-threads to provide an experimental indicators, from the perspective of Chinese medicine clinical different dialectical provide the basis for the diagnosis.7. With photoelectric amplitude of the blood volume increased gradually increased in HbO2, ET has been gradually declining, NO increased gradually, TXB2 and has been gradually declining,6-K-PGF1αincreased gradually, GMP-140 is gradually declining. And low volatility and high volatility group there were significant group differences.With photoelectric amplitude of the blood volume increased gradually increased in HbO2, ET has been gradually declining, NO increased gradually, TXB2 and has been gradually declining,6-K-PGF1αincreased gradually, GMP-140 is gradually declining. And low volatility and high volatility group there were significant group differences.Photoelectric flow volatility and the volume of the main indicators related to chemical analysis found:HbO2 content with a positive correlation with the content of ET negatively correlated with NO content is positively correlated with the content of TXB2 negatively correlated with 6-K-PGF1αin a positive correlation with the GMP-140 in a negative correlation, all of which have very good the relevance of clarifying the photoelectric blood flow volume based on the physiological and pathological formation mechanism. At the same time in the blood stasis, phlegm card, the normal group among the three groups, photoelectric blood volume amplitude of the main significant difference in the HbO2, ET content, NO content in TXB2,6-K-PGF1αcontent, GMP-140 in comparison group were significantly different. The results show that:Different type of blood flow volume parameters photoelectric formation of pathological and physiological characteristics.
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