The Influence Of Control Factor In Rats With Brain Edema And Cell Apoptosis After Acute Intracerebral Hemorrhage Treated By Electro-Nape Acupuncture Therapy

Objective:To research the mechanism of control factor in which electro-nape acupuncture therapy influences the brain edema and apoptosis occurred from intracerebral hemorrhage,through the experiment on the rats with intracerebral hemorrhage.Method:healthy male wistar rats(weight:250-300g) were randomly divided into three groups:A,B and C,each group was again randomly divided into four smaller groups;12h,24h,3d,7d four time points,the rats were prepared by rules.The rats in group A were injected 0.9%physiological saline 2ul,the rats in group B and C were injected 0.9% physiological saline with 0.5u/ul collagenase 1ul and 0.9%physiological saline with 7u/ul heparin 1ul,then observe the information and data at assigned time:Neurological deficit scale,the brain tissue water content,braintissue patho-form,braintissue ultramicro-texture and AQP-4,MMP-9,Caspase-3 and Bcl-2 by immunity histochemistry method.Results:1.the recordings indicate there is Neurological deficit scale in hemorrhage rats,the peak occurred at 3d and descent then.There are significant disparity between model group and treating group.2.The brain tissue water content step up after 24h and the peak occurred at 3d,there are significant disparity between model group and treating group.3.Light-microscope shows there's severe pathological change in brain hemorrhagic model group,Nerve cells and colloidal cells swell,and in cell space there's vacuoles of different sizes and the cell space increases.In treating group,hemorrhage and cell dropsy are distinctly alleviated.4.Electron microscope results show that the cerebral ultrastructure was significantly meliorated in treating group;There were plenty of golgicomplexes in neuron,the structure of mitochondrion and other cell organs were full and there were more synaptic vesicles in treating group.5.The AQP-4 expression in model group reach peak at 3d,there are significant disparity between model group and treating group(P＜0.01).6.The MMP-9 expression in model group reach peak at 3d,there are significant disparity between model group and treating group(P＜0.01)7.The Caspase-3 expression in model group reach peak at 24h,there are significant disparity between model group and treating group(P＜0.01)8.The Bcl-2 expression in model group reach peak at 24h,there are significant disparity between model group and treating group(P＜0.01)Conclusion:1.Electro-nape acupuncture therapy reduces Neurological deficit scale in hemorrhagerats,improves the nervous function.2.Through observing and comparing braintissue morphology around hematoma,BBB ultramicro-texture,ultramicro-texture of braintissue-edema zone,Electro-nape acupuncture therapy improves pathological change of braintissue-ultramicrotexture.3.The above results show that the content and changes of AQP-4 and MMP-9 have big relationship with cerebral edema after hemorrhage,they may lead to permeability in BBB and increasing brain tissue water.4.The above results show that the content and changes of Caspase-3,Bcl-2.have big relationship with cerebral apoptosis after hemorrhage.5.The symptom became better after electro-nape acupuncture therapy,one of the mechanism of anti-edema and anti-apoptosis by acupuncture may adjust the expression of AQP-4,MMP-9,Caspase-3 and Bcl-2,diminish break in BBB,degrade brain water content,improve symptom accompanied with cerebral edema and apoptosis.