Studies On Guizhi Fuling Pills Decoction To Probe Into Semi-bionic Extraction Mode On Compound That Contains Volatile Ingredients

Objective:Take Guizhi Fuling Pills Decoction as an example to probe into the basic mode on extraction of available substances in Chinese compound medicine that contain volatile ingredients by semi-bionic extraction(SBE).Methods:Firstly,Guizhi Fuling Pills Decoction was studied on literature,the source and contents identification of cut crude drugs.Of extraction study,First,Using U₇(7⁴)homogeneous design and taking cinnamaldehyde,cinnamic acid,paeonol and total CO₂ extract yield as evaluating indexes to optimize SFE-CO₂ technical conditions for extracting volatile ingredients of Guizhi Fuling Pills Decoction,Then,mixed dregs of medicinal material extracted by SFE-CO₂ with other medicinal material,using U₉(9¹Ã—3³) homogeneous design form and taking Paeoniflorin,Cinnamic acid,Laetrile,Pachymic acid, total polysaccharose and dry extract as indexes,the result was comprehensive evaluated to optimize SBE technical conditions.Next,basede on the optimized condtions,using proportional method to optimize the alcohol precipitation concentration of SBE extracting solution and WE extracting solution and alcohol extraction concentration.Last,the 5 kinds of extracting solution(SBE,WE,SBAE,WAE,AE) were comprehensive compared through the multi-indexes ingredients and HPLC fingerprints chromatograms.Because the same components content not mean the same biologic effects,so we contrasted 5 kinds of extracting solution on effect of hemorrheology of the acute blood stasis rats model,effect of dysmenorrheal rats induced by drug,anti-inflammatory action, immuneomodulation effect and Cyclomastopathy rats model.And acute toxicity was compared.Simultaneously,compare the 5 kinds of solution and medicated serum on contractility of isolated rat uterus in the presense of oxytocine.Finally judged them through comprehensively evaluation and determined the best technique method and technique parameters of Guizhi Fulingpills Decoction.According to the former extraction study,optimizing the shaping technology of Guizhi Fuling soft capsules and establishing quality standard.Results:(1)The optimized SFE-CO₂ extraction conditions are the following:the extraction pressure was 30 MPa;the extraction temperature was 36℃,the separation temperature was 32℃and the extraction time was 3.5h.(2)the optimized SBE extraction conditions are the following:pH values of the water for three extraction is 5.0,7.5,8.0,and extraction time is 2.0h,1.5h,1.5h in turn.(3)the optimized alcohol precipitation concentration for SBE solution is 60%.(4)the optimized alcohol precipitation concentration for WE solution is 70%.(5)the optimized alcohol concentration is 70%. (6)the order of comprehensive evaluated values of the 5 kinds of extracting solution is Y_SBE＞Y_AE＞Y_WE＞Y_SBAE＞Y_WAE.(7)SBE solution has the most total area of corresponding characteristic peaks in the fingerprint chromatograms of 5 kinds of solution.(8)in the serum fingerprint chromatograms of 5 kinds of solution,the SBE solution has the most peak number and total area.(9)Main pharmacodynamic activities test results indicate that the comprehensive value of SBE extract is the largest among extracts by 5 methods and no animals died in maximum tolerated dose experiment.(10)The 5 kinds of solution and their medicated serum could certain degree inhibit the contractility of the isolated uteri,and SBE solution and it's medicated serum has the max comprehensive value.(11)Optimizing the shaping technology of Guizhi Fuling soft capsules and establishing a quality standard for Guizhi Fuling soft capsules so as to control its quality efficiently and effectively.Conclusion:The best way to prepare Guizhi Fuling Pills Decoction is as follows,first, extracting Herbal pieces With volatile components(Ramulus Cinnamomi,Cortex Moutan, Radix Paeoniae Rubra and Semen Persicae)by SFE-CO₂,then mix dregs with other medicinal materials(Poria Cocos),and extracted by SBE.Compared with WE,SBAE, WAE and AE method,Guizhi Fuling Pills Decoction extracted by SBE possesses more contents of chemical compositions and better pharmacodynamic functions.This study verified again the scientificity,rationality and advancement of the semi-bionic extraction patterns for compound which based on the gray thinking mode.Provides reference to other researches of valuable substances containing volatile components in traditional Chinese medicine and twice exploitation of chinese herbs.In the meantime,it may open a new avenue for the rapid growth of Modernization of Traditional Chinese Medicine.