Studies On Cihang Dan Prescription To Probe Into The Semi-bionic Extraction Mode On Comound Containing Volatile Ingredients

Objective:Taking Cihang dan prescription as an example to probe into the basic mode on extraction of available substances through the final product of molecular weight≤1000 Da contraction of traditional Chinese medicines compound containing volatile ingredients based on the composite evaluate indexes by comprehensive evaluation extracted by semi-bionice extraction (SBE) method.Methods:Fistly, distinguishing the source,the character and definiting the contents identification of the prepared slices of Chinese crude drugs respectively according to Chinese pharmacopeiachp of 2005; only if they are fit for the pharmacopoeia quality standard, they can be used in the experiment. Secondly, extracting the volatile ingredients of Rhizoma Chuanxiong,Rhizoma Cyperi,Radix Angelicae Sinensis by supercritical carbon dioxide extraction (SFE-CO2) through U7(75) homogeneous design based on the same conditions of medicine,equipment and technical parameter and taking ligustilid,chuanxionggzine hydrochlorid,α-Cyperone and toal yield as evaluating indexes to optimize four operational variables of SFE-CO2 (extraction pressure,extraction temperature,desorb temperature and extraction time); the total productivity of SFE-CO2 extraction was stored in other container. Thirdly, getting the dregs of three medicinal material extracted by SFE-CO2 and Herba Leonuri together and extracted by SBE through U9(91×33) homogeneous design based on adding all the indexes of the productivity of Chuanxionggzine hydrochlorid,Ferulic acid,Stachydrine Hydrochoride,D-Glucose anhydrous and molecular weight≤1000 Da dry extraction processed by standardized, comparing the composite evaluate indexes of each method by weighted summ and finally choosing the best method of the preparation of molecular weight≤1000 Da contraction. Fourthly, Optimizing the best consistency of ethanol(30%-80%) for extracting the prescription by proportional subsection method through the same method as mentioned above to ge the final product of molecular weight≤1000 Da contraction.Fifthly, extracting the Cihang dan prescription by SBE,by water and eighty percent of ethanol respectively, got the there contractions of molecular weight≤1000 Da and optimized the best method by comparing the composite evaluate indexes through the same deal methods.Sixth, as the matter of the same component not mean the same effect, comparing the results of studying on the main pharmacodynamics and the acute toxicity test including the effects of dysmenorrhea,regulate menstruation,neuroleptanalgesia and the toxicology.Seventh, comparing the results of High Performance Liquid Chromatography (HPLC)fingerprint of Cihang dan prescription extracted by three methd through the contractions of molecular weight≤1000 Da and the serun containing drugs of rabbits.Eighth, comparing the effects of serun containing drugs of molecular weight≤1000 Da of rabbits on the smooth muscle of isotated uterine in the rats of Cihang dan prescription extracted by three methods.Finally, Optimizing the best conditions of Cihang dan prescription extracted by three methods by composite evaluate indexes of each method by weighted sum.Results:①All the prepared slices of Chinese crude drugs of Cihang dan prescription were fit for the pharmacopoeia quality standard of 2005 respectively and can be used in the experiment.(2)Combinating manufacturing fact, the optimal extracting procedure was as follows:the extraction pressure is 30Mpa; the extraction temperature is 48℃, the desorb temperature I is 60℃, the desorb temperature II is 30℃and the extracting time is 4.0h.③The best conditions of Cihang dan prescription is that the pH value of three extractions is 6.0,7.5,8.8 respectively and the extraction time is2.0h,1.5h,1.5h in turn.④The best consistency of ethanol for extracting the prescription is 80 percent.⑤The composite evaluate indexes of Y of each method by weighted sum of CihangDan prescription extracted by three methods is as follows:YSBE>YAE>YWE.⑥Haven had optimized the best sampling blood time of the rabbits was 1.5h by the HPLC fingerprints of the serun containing drugs of rabbits of Cihang dan prescription extracted by SBE and the dosage of the rabbits was 7.0g·Kg-1 (crude drug-body weight-1).Facts proved that the characteristic peak numbers and the total areas of SBE group both were the most by comparing the results of the HPLC fingerprints of the serun containing drugs of rabbits and three contractions of Cihang dan.⑦The results showed that the resultant effects of promoting blood circulation to restore menstrual flow,acesodyne and anti-inflammatory of SBE extraction is the best through the experiments of elementary pharmacodynamics and the results were in agreement with that of comprehensive evaluating by multi-indexed process and the research result of HPLC fingerprints of Cihang dan prescription extracted by three methods, but the research result of toxicology of the three extractions were not statistical significance differentially, none of the rats was dead in the test.⑧Comparing to the control group, all the serun containing drugs of the extractions of the Cihang dan prescription extracted by three methods can significantly reduce the contraction of the amplitude,the frequency and the tension of the rats'in vitro uterine smooth muscle (P<0.05); Comparing to the SBE groups, the AE group and the WE group both were statistical significantly differentially(P<0.05), and of which the SBE groups produced the greatest effect (P<0.05).⑨It proved that the method of SBE is the best one of three methods through composite judgement.Conclusion:The best way to extract the medicinal composition of Cihang dan prescription as follows:firstly extracting herbal pieces with volatile components(Rhizoma,Chuanxiong,Rhizoma Cyperi,Radix Angelicae Sinensis)by SFE-CO2, then blending dregs of decoction Herba Leonuri and extracted by SBE method and got the molecular weight≤1000 Da contraction through "centrifugate-membrane filtration method".It proved that the comprehensive components value of 3 kinds of extraction solution(SBE,AE,WE)consistent with comprehensive pharmacodynamics value.Of the three extractions of the Cihang dan prescription extracted by there methods, the content of pharmacodynamic substances of SBE extraction was the highest and the effect of the pharmacological action of SBE extractions was the strongest, among the three extracting methods, the SBE method was the best. In the study, all the indexes of the chemical composition,the productivity of the (molecular weight≤1000Da) dry extraction,the elementary pharmacodynamics,the HPLC fingerprints of the extractions of the Cihang dan prescription and the results of the HPLC fingerprints of the serun containing drugs of rabbits of the three extractions of Cihang dan prescription extracted by there methods were organically combined together systematically.The study further richened and embodied the rationality and the scientificalness of the basic mode of establishing semi-bionic extraction method of Chinese medicine by gray thinking method.The study embodied the globality,synthesis objectivity and fuzzyness of Chinese medicine through the results of molecular weight≤1000 Da contractions.The study provides reference to other researches in choosing modern pharmaceutical dosage form and expanded the train of thought and method for the study of modernization of Chinese medicine.