Based On ALD To Investigate The Regulating Mechanisms To Endocrine System Of SQW

The formula of Suoquanwan(SQW) is recorded in a Chinese medicine book named Furenliangfang,which collected hundreds of formulas of Chinese medicine combinations.SQW consists of three herb medicines,Radix Linderae,Radix dioscoreae,and alpinia oxyphylla Miq.The function of SQW is to warm kidney,stop polyuria and hold excessive urination.In clinical practice,SQW is used for the treatment of urinary system diseases,which is polyuria and excessive urination due to Yang-deficiency of Kidney,such as enuresis,diabetes insipidus, nocturnal enuresis,neurogenic urinary frequency,Chronic glomerulonephritis,Nephrotic syndrome,Urethral syndrome.According to TCM's theory,polyuria is closely related to the function of Kidndy and bladder,that is,warm kidney and hold excessive urination can effectively cure the disease.Investigation in the earlier stage showed that its function of anti-diuresis was connected with retaining sodium and arranging potassium,and also promoting the combining and secretion of aldosterone,adjusted by Angâ…¡,ACTH,which was involved in endocriniums,such as RAAS,HPA.In endocriniums,HPA can affect the function of kidney,and HPA and RAAS can also adjust liquid metabolism by Angâ…¡,ACTH's affection to the combinign and excretion of ALD.According to the above analyse,focus on the combining and secretion of aldosterone,the thesis chose SQW to act to warm kidney and hold excessive urination and compared the concentration and contents of PRA,ACE, Angâ…¡,ALD,ACTH,cAMP in blood when Yang-deficiency of Kidey with after oral administration of SQW,and also explored its mechanism of affecting the combining and secretion of aldosterone,established the target spots by observing the mRNA and protein expressions of AT1R,MR,ACTH, GYP11B2.Through the above experiments,the thesis provided molecular pharmacology mechanism for SQW and expounded its therapy characteristics of multisystem and multi- target spots.The thesis explored SQW's therapy effect and adjusting function to endocriniums in kidney-yang deficiency rats with polyuria by detecting the weight of thymus gland,spleen,adrenal gland,hypophysis,urine and the concentration of ions in urine.By observing pathology morphology change of the adrenal gland,hypophysis and the contents change of CRH,Cort,cAMP,ACTH,PRA,ALD,Angâ…¡,ACE in blood,the study expounded its adjusting function to HPA,RAAS and the compound of ALD.The investigation also revealed SQW's molecular pharmacology mechanism after detecting mRNA and protein expressions of AT1R,MR,ACTH,CP11B2 by the mothed of RT-PCR and immunohistochemistry.The results show that SQW reduces significantly the urine and the concentration of sodium,chlorine in urine,and elevate urine potassium excretion in kidney-yang deficiency rats with polyuria.SQW can improve the atrophy state of thymus gland,spleen,adrenal gland,hypophysis by increasing its weight.The contents of CRH,Cort,ACE,Angâ…¡,ALD,ACTH, cAMP increase after SQW administration in model rats.The pathological characteristics of kidney and adrenal gland,hypophysis of model rats are switched.By RT-PCT and immunohistochemistry,both mRNA and protein expressions of AT1R,MR,ACTH,CYP11B2 are increased.The thesis expounds the pharmacology mechanism of SQW in nourishing kidney and reducing urine.At first,SQW can promote the combining of ALD by increasing the content of Cort,and also adjusting the mRNA expression of CYP11B2.The target is related to CYP11B2.And then,SQW can treat kidney-yang deficiency with polyuria by affecting endocriniums,which includes two approachs.One is that SQW can hold excessive urination by adjusting the combining and excretion of ALD.Its mechanism is to adjust HPA by changing the content of CRH,ACTH,cAMP in blood,improving the pathological characteristics of adrenal gland,hypophysis and by so doing,its function of nourishing kidney is correspondingly enhanced.Besides that,SQW can improv immune function by increasing the weight of imune organs,which also lead to the enhancement of HPA'effect.In short words,SQW's nourishing kidney is mainly connected with the above two factors.Its target spot in HPA is related to ACTH.Another is that SQW adjusts RAAS by affecting the contents of PRA,ACE,Angâ…¡in blood.And its target spots in RAAS are connected with AT1R,MR.The thesis confirms the therapy's characteristics of SQW,which are multi-target,multi-system,integral regulation,and also expounds its connotation of nourishing kidney and reducing urine,which involved in RAAS,HPA and MR,AT1R,CYP11B2,ACTH.