The Research Of Pain Suppressing And The Immune Mechanism Caused By Electro Acupuncture Therapy On Primary Dysmenorrhea Rats

1.Reserch purposeDysmenorrhea is a common disease in the department of gynecology and can be treated effectively by acupuncture and moxibution.Its pathogenesis is very complicated and probably close to endocrine unbalance abnormal uterine contractility,uterine ischemia of hypoxia.The subject of this article is to explore the new development of TCM treatments on dysmenorrhea application. In recent years there have been many reports on the use of acupuncture in treating PD,because its applications are simple,effective,and with few side effects it has received widespread attention from the medical community. Hopefully by addressing this issue,we can further investigate treatment of PD by building on previously accumulated research and experience,to find a method of treatment that is safe,effective,and can be repeated.2.Method2.1 Literature ResearchAdopt documents researching approach to explain synthetically the understanding of primary dysmenorrhea mechanism and commonly used methods of treating dysmenorrhea in accordance to symptoms,the TCM understanding of primary dysmenorrhea,and treating dysmenorrhea with acupuncture therapy and moxibustion,and to comment and look into the distance.2.2 Experimental ResearchThis research regards the equilibrium ability of the PD organism as the breakthrough point;adopts methods include biochemistry,radioimmunology etc; observes and appraises,the effects of electro acupuncture therapy under different groups with individual levels which are a whole,a system,an organ, and a molecule and compares and contrasts it to the effects achieved by Western medication.(1) The animals tested upon and grouped as of the following:40 SD female rats of clean class,were divided randomly into four groups,the blank group, the model group,the electric needle group,and the medication group,with each group containing 10 rats.(2) Establishing the PD animal model:Make the model by injecting 0.8mg of diethylstilbestrol injection per rat(1^st and 10^th day),0.4mg per rat(from 2^nd day to 9^th day) into the abdominal cavity and 2U of oxytocin injection per rat on the 11^th day.(3) Method of choosing electro acupuncture prescriptions:According to the traditional theory under TCM of the Ren meridian,Chong meridian, prescription composed of Guanyuan(RN4),Sanyyinjiao(SP6).(4) Medication group:Make model by feeding 0.8ml of Ibuprofen sustained-release capsules per rat for 10 days in succession.(5) Observe general behavior and amount and percentage of total found squirming.(6) Observe PGE₂,PGF_2a contents of endometrium of the PD model rats under the different groups.(7) Observe TNF-α,IL-6,IL-1 contents of serum of the PD model rats under the different groups.(8) Observe NK cell activeness in the spleen of the PD model rats under the different groups.(9) Observe 5-HT contents of the hypothalamic organization of the PD model rats under the different groups.(10) Observe the change and influence of electro acupuncture on the above mentioned in order to probe into its function and mechanism of treating PD.(11) Analyze the meaning of the change of the above mentioned and its relevant index and interaction between meaning and index in which the above mentioned' s relevant indexes change.3.Result(1) The PD model rats appeared with slow movement and reaction,dispirited spirit,weight increase getting slow,the hair color turned yellow and the gloss was bad.Electro acupuncture could improve this status.(2) Not only electro acupuncture but also western medicine could improve body-stretching rate. (3) To observe endometrium contents of PGE₂,PGF_2a,the ratio of PGF_2a/PGE₂ of the PD model rats under the different groups,there exists an obvious difference.There is no obvious difference between the blank group,electro acupuncture group and medication group but electro acupuncture group and medication group.(4) The electro acupuncture can improve the serum contents of TNF-α, IL-6,IL-1 and spleen NK cell activeness and hypothalamic organization contents of 5-HI under the PD model,enhance the function of immunity systems. The pain release effect is the same as western medicine and achieved the normal rats standard.(5) To observe the response to the PD rats under different groups,use the TCM principles with acupuncture,which provide experimental data for clinical application and a basis for further study.4.Conclusions(1) The model was made successfully according to the observation of general behavior and amount and percentage of total found squirming.(2) The electro acupuncture can improve the level of PGE₂,PGF_2a,TNF-α, IL-6,IL-1,TNF-α,IL-6,IL-1 and 5-HT.This research provides experimental data for clinical application of treating PD by electro acupuncture and a basis for further study.There is abundant experience on PD treatment under TCM.To use the acupuncture and moxibustion to treat PD is very special and strong.It is cheaper,easier,non-poison etc.