Influence On Rat Model With Renal Deficiency By Invigorating Kidney Therapy

Objective:The research focused on invigorating kidney therapy applying on rat model with renal deficiency and ovulation inhibition built by Hydroxyurea for stimulating ovulation.The therapies for invigorating kidney are including herbal medical formula, acupuncture,both herbal formula and acupuncture.This study would make sense in mechanism of Chinese medicine for female infertility treatment on ovulation inhibition.Method.SPF SD female rats,10-12weeks old,weight 220-250g,were made by Hydroxyurea for stimulating ovulation.After intragastric administration of hydroxycarbarnide,Vaginal cells in smear and pathological changes of ovary were observed.Successful model has prompted by inhibiting ovulation pathology,which the proportion of secondary growing follicles and mature follicles was reduced in evidence,primordial follicles and primary growing follicles were in the majority in ovary when the numbers of follicles of all levels were counted through light microscope in the 29^th day.Successful model rats were distributed into 4 groups at random,which were group A:model,B:model +medicine,C.model +acupuncture,D.model + medicine + acupuncture,adding E:control,which were not intragastric administration. There were 5 groups all.Rat model with renal deficiency were given invigorating kidney therapies,including herbal medical formula,acupuncture,both herbal formula and acupuncture.Herbal medical formula and(or) acupuncture intervention in the first 6 days,half of each group rat were taken female and male with cage;In the first 15 days,rat which were not with the male rat with cage were killed in asepsis condition, and the following date was measured:blood-serum sex hormones and inhibin,wet weight of uterine,ovarian,kidney,spleen,adrenal and pituitary.At the same day,rat which were with the male rat with cage were taken out of the cage.After 7days,rat which were with the male rat with cage were killed in asepsis condition,the following date was measured:blood-serum sex hormones,wet weight of organs,pregnancy rate.Results,1.Model rat with intragastric administration of hydroxycarbamide expressed symptoms of renal deficiency,less activity,slower weight increase,weight of model rats were lower compared with control group.2.Invigorating kidney therapies were taken on successful model rat with renal deficiency and ovulation inhibition,involving promoting ovulation herbal medical formula of Luo,acupuncture of Guanyuan,Zhongji,Shenshu,Zigong points,both herbal formula and acupuncture.Wet weights of uterine and ovarian were increased in varying degrees compared with model group,but wet weights of kidney,spleen, adrenal,pituitary did not increase in significant difference.The pregnancy rates,the number of pregnancy embryos and the diameter of a single embryo were also increased.3.There were no significant difference in blood-serum sex hormones and inhibin A and B in all groups(P＞0.05).Conclusion:Conclusions were educed on research of invigorating kidney therapy applying on rat model with renal deficiency and ovulation inhibition built by Hydroxyurea for stimulating ovulation.1.Model rat with intragastric administration of hydroxycarbamide were successfully built,symptoms of deficiency both renal and spleen were showed.2.Nourish kidney therapies effectively improved the symptoms of deficiency both renal and spleen after treatment.3.Nourish kidney therapies involved promoting ovulation herbal compound medical formula of Luo which has radix aconiti carmichaeli,semen cuscutae,radices morindae,herba epimedii,and so on,acupuncture of Guanyuan,Zhongji,Shenshu, Zigong points and both herbal formula and acupuncture.Pregnancy rate,number of pregnancy embryos,diameter of a single embryo of treatment groups improved effectively.But there were no significant difference in organ wet weights, blood-serum sex hormones and inhibin in all groups.4.There were no significant difference in organ wet weights,blood-serum sex hormones and inhibin in all groups,which could illustrate model rat with intragastric administration of hydroxycarbamide,which were at the early reproductive life,did not influence the reserve capacity of ovarian..