The Effects Of Qinggan Xiehuo Prescription On Graves Disease Rats About The Expression Of Inflammatory Factors And Related Proteins

Objective:Graves Disease(GD) model is established by immuning Wistar rats with Y.E.to draw analogy between the curative effect of the decoction that subdue the pathogenic fire in liver in different dosage,discuss whether the composition of the decoction that subdue the pathogenic fire in liver is reasonable,verify the hypothesis that the occuring of GD is closely related to the pathological injury caused by partial inflammation in the thyroid gland and expond the mechanism of treating GD through subduing the pathogenic fire in liver.So as to find new methods for the bianzhengshizhi of GD,and lay the theoretical foundation and experimental evidence for the further development of new drugs.Method:Take immuned Wistar rats with Y.E.as the experimental model, then divide the rats into 5 groups randomly:1.model group;2.group of small dosage of herbal medicine;3.group of medium dosage of herbal medicine;4.group of large dosage of herbal medicine;5.group of thiamazole;treat them with small,medium,large dosage of herbal medicine and western medicine respectively.And TH is examined by RIA,NF-κB is examined by Immunohistochemistry Technique,CK is examined by RT-PCR,CRP and ICAM-1 is examined by ELISA. Result:1.Result of TH of the GD rats:The Tsh of the rats in model group decreased obviously,and t he FT₃,FT₄ of the rats in model group rose significantly,and the re were obvious difference between the normal group and the mod el group,indicating that the model was successfully established (P<0.01).It was of significant difference when the group of we stern and the groups of herbal medicine were compared with the model group(P<0.01),which indicated that the curative effect was remarkable and played the role of anti-thytoglobulin.And th ere was no obvious difference between the group of western medi cine and the groups of small,medium dosage of herbal medicine(P >0.05),and the large-dosage of hebal medicine was quite differe nt.Curative effect of small-dosage,medium-dosage of hebal med icine and western medicine group was quite basic and large-dosa ge was significantly better than the other groups(p<0.01).2.Result of CRP of the GD rats:The CRP of the rats in model group rose obviously,which was significantly different from the model group(P<0.01),indicating that the occurance and development of GD were closely related to the inflammation.There were obvious differences between the treating groups of small,medium and large dosage of herbal medicine and the model group(P<0.01).And there were also obvious differences between the group of small,large dosage of herbal medicine and the group of mediun dosage of herbal medicine(P<0.01).The group of medium dosage of herbal medicine was more superior than the group of large dosage of herbal medicine and the western medicine(P<0.01). The result indicated that the curative effect of the group of medium dosage of herbal medicine was more remarkable than the group of large dosage of herbal medicine and the western medicine group.The fluctuation of TH of the rats in the groups of small,medium and high dosage of herbal medicine did not synchronise with the fluctuation of CRP,indicating the fluctuation of CRP beared no obvious relation to the fluctuation of TH.So,whether CRP is an independent factor of the occurrence and development of GD should be studied further more thoroughly.3.Result of ICAM-1 of the GD rats:The ICAM-1 of the rats in model group rose obviously,which was significantly different from the normal group(P<0.01).There were obvious differences in ICAM-1 between the treating group of small,medium,large dosage of herbal medicine and the model group(P<0.01).Apart from the above mentioned,there were no significant differences among the treating groups of herbal medicine(p>0.05),and they were also different from the western medicine group(p>0.05),indicating that the treatment groups have no different in treatment effect.4.Result of NF-kB of the GD rats:The NF-κB of the rats in model group rose obviously,which was significantly different from the normal group(P<0.01).The pathological result showed that the density of TEC of the rats in model group increased obviously,the majority of positive signals existed in nucleus and cytoplasm at the same time.And there was lots of brown particles in the cytoplasm,ranging in slices, indicating that there were obvious differences in NF-κB between the treating group of small,medium,large dosage of herbal medicine and the model group(P<0.01).The treatment group intracytoplasmic accumulation of brown granules scattered,suggesting the group of herbal medicine treatment in all dosage groups was more apparent. There was a significant difference between its large-dosage group and small-dosage group medicine in the dosage of herbal medicine, (P<0.01),but no difference with the western medicine(p>0.05).In treatment effect,the large-dosage group was better than the small and medium dosage group.5.Result of IL-6mRNA,IL-10mRNA of the GD rats:The IL-6mRNA of the rats in the model group rose obviously,with a brightest light band,and its average of OD was significantly different from the normal group(P<0.01).ODs in each treating groups fell obviously,and was significantly different from the model group(P<0.01).There were also of great difference among the group of medium dosage of herbal medicine,the group of western medicine and the group of large dosage of herbal medicine(P<0.01),indicating that the curative effect of the group of medium dosage of herbal medicine was better than the group of large dosage of herbal medicine and western medicine.The IL-10mRNA of the rats in the model group decreased obviously, and was obviously different from the normal group(P<0.01).There were significant differences between the treating groups and the model group(P<0.01).There were significant differences between the large-dosage group and small-dosage group,medium-dosage group, the western medicine group.And the large-dosage group of herbal medicine had the best effect.6.Result of TNF-a of the GD rats:The TNF-αof the GD rats in the model group rose obviously, which was significantly different from the normal group(P<0.01).There existed obvious differences between the treating groups of small,medium,large dosage of herbal medicine and the model group(P<0.01).No significant difference was found in each treating group(P<0.01).There was significant difference between the treating groups and the normal group(P<0.01).This result directly verified that TNF- a took part in the immune response.With the controlling of the disease,the content of TNF-αfell obviously and its fluctuation was not synchronical with the ones of FT₃,FT₄,TSH.It indicated that TNF-αcould not be the independent factor of the cause of GD while the disease was the result of the pathogenic factor in many collaborative.Conclusion:1.The GD model established by injecting Y.E.into tail vain of rat shows the major character of GD in animal.This method is effective and advanced in researching of medicine and pathology.2.The decoction that subdue pathogenic fire in liver can improve TH level,and play the role of anti-thyroid.3.The high level of inflammatory protein and inflammatory factors in the rats in GD model group thoroughly proved that GD is closely related to inflammation.4.The decoction of subduing pathogenic fire in liver can control and improve the condition of disorder like the inspects saying above.