Hypericum Attenuatum Choisy To Ischemic Heart Disease Beastie Model Drug Action Material Basis And Mechanism Of Action Of Disquisition

There have been many people lost their life because of cardiovascular disease at home and abroad. Cardiovascular disease has become a major cause of morbidity and mortality throughout the world. And it affected people's heath and life quality seriously. Ischemic heart disease has a close relationship with XIONG BI and XING TONG of Chinese Medicine. There exist a lot of advantages in treating ischemic heart disease with Chinese medicine.Hypericum attenuatum Choisy. , a medical plant in genus Hypericum, was known as WEN XIN CAO in some area and it was used to treat hear diseases as a kind of drink. Recently, some compounds were seprated from it such as hyperin, hyperoside, quercetin, etc. But at present, the concrete experimental study of Hypericum attenuatum Choisy. on cardiovascular disease has not been found. This article mainly studied the effective chemical composition group of Hypericum attenuatum Choisy. that had obvious effect on ischemic heart disease.First, pharmaceutical research established tow kinds of detection methods that could detect chemical composition and established the abstraction method that could make total flavonoids separated from Hypericum attenuatum Choisy.Second, drug efficacy study was used. The results indicated that:1.The total flavonoids of Hypericum attenuatum Choisy. could inhibit the cardiac arrhythmia induced by Calcium chloride in rat, which also could increase the myocardial contractility, decrease heart rate of isolated heart and promoted the isolated heart rate have a stable law.2. Coronary artery ligation experiment showed that the total flavonoids group could remarkably decrease the activities of serum CK-MB and LDH. It also significantly increased the activity of SOD and decreased the levels of MDA in myocardium.3. The ultrastructure was observed by electron microscope. The result indicated that compared with model group, the myocardial cell and chondrosome of positive control group and total flavonoids group had little changes.4. Immunohistochemistry study showed that the total flavonoids of Hypericum attenuatum Choisy. could depress or disrupt the procedure of cell apoptosis by regulating the expression of Bcl-2 gene. At the same time, the expression of Bcx gene could be detected in all groups, and non-flavonoids groups' were found to be more obvious.To conclude, the main material basis that Hypericum attenuatum Choisy. treat experimental ischemic heart disease was the total flavonoids...