| Object : Syndromes theory is the core theory of Chinese medicine theory . Syndromes animal model is a powerful tool for revealing syndrome biological basis . The overall and dynamic characteristics of Proteomics is surprisingly similar with the "whole concept" and the "concept of differentiation"of Chinese medicine theoretical system. Based on the above understanding, the rat model of left anterior descending coronary artery ligation is applied and judged by Syndrome property assessment from macro-performance, tongue, cardiac function,laboratory indicators and the efficacy of traditional Chinese composite prescriotion .The model of myocardial ischemia was sured to belongs to Qi-deficiency and Blood–stasis Syndrome . We discuss the pharmacological mechanism of replenishing Qi and activating Blood composite prescriotion to reverse myocardial fibrosis . And we applied proteomics technology to detect the cardiac different protein group between sham-operated group and the model group to reveal the biological basis of Qi-deficiency and Blood–stasis Syndrome.Methods :1 Methods of constrcting model and collecting four diagnostic information: left anterior descending coronary artery ligation rat model was applied.3 days after surgery , 4-6 abnormal Q–wave showed that coronary ligation is successful,Successful models were randomly divided into three groups, model group,Replenishing Qi and Activating Blood (RQAB)group and Warming yang to excrete water(WYEW) group,while producing a sham -operated group (not ligation of the left anterior descending coronary artery, and the rest is the same with model group).3 days after surgery, sham-operated group and the model group were stomach-perfused for 10ml/kg.d drinking water , RQAB group for 0.1g/kg.Qishenyiqi Pills (the main component of astragalus root, red sage root, notoginseng. dalbergia wood) WYEW group for 0.396g/kg.d Zhen Wu decoction. The experimental cycle is 30 days. At the end of the experiment, the number of each group sample is 10.At the 7,14,21,28 day of postsurgery,we observed and detected the rats of the general state, the tongue, fingernail and auricular, body weight, body temperature, respiratory rate and ECG. At the 8,15,22,29 day of postsurgery,we detected the rats, exhaustive swimming time (6% in heavy load ,temperature of 30±1°C water swimming, Exhaustive in terms of time, sinking into the water in the first 10 seconds as the exhaustive identification).At the 30 day of postsurgery ,the rats echocardiography was detected. At the 32 day of postsurgery ,the rats were killed, and the tongue tissue was dealed with HE staining, and the ET and CGRP of plasma was detected by RIA.Myocardial tissue were dealed with HE and Masson staining . The expression of TGF-β1 was detected by immunohistochemical staining, and the TNF, IL-6, Ang II, Ald of rat myocardial tissue were detected by RIA.2 Methods of detecting myocardial Proteomics : At the 32 day of postsurgery, three rats were randomly selected in the model group and the sham-operated group.The rat of model group chose the myocardial tissue of ischemic area ,and the rat of sham-operated group normal left ventricular The above myocardial tissues were dealed with the two-phase protein electrophoresis,silver staining and protein mass spectrometry testing.Result:1 the evaluation of syndrome : At the 8,15,22,29 day of postsurgery, compared with the sham-operation group ,model group ,s exhaustive swimming time decreased significantly; At the 22,29 day of postsurgery, RQAB group and WYEW group,s exhaustive swimming time increased significantly compared with model group. The model group ,s tongues were dark and purple compared with the sham-operation group, and R, G, B and L value of the model group ,s tongues dropped significantly ; At the 21,28d of postsurgery, L value of RQAB group increased significantly compared with the model group . Compared with the sham-operated group, the micro-vein diameter of the model group increased significantly ; Compared with the model group ,the micro-vein diameter of RQAB group decreased significantly (P <0.05). Compared with the sham-operated group, ET of the m odel group increased and CGRP decreased; Compared with the model group ,ET and CGRP increased . Compared with the sham-operated group ,EF and FS of the m odel group decreased ; Compared with the model group , EF and FS of the RQAB group increased the model group and the sham-operation group, dropped significantly.Compared with the sham-operated group, TNF, IL-6, Ang II, Ald of model group increased significantly; Compared with the model group , TNF, IL-6, Ang II, Ald of the RQAB group decreased . Compared with the sham-operated group, CVF of model group increased significantly; Compared with the model group , CVF of the RQAB group decreased . Compared with the sham-operated group, the expression of TGF-β1 of model group increased significantly; Compared with the model group , the expression of TGF-β1 of the RQAB group decreased .2 proteomics results of Qi-deficiency and Blood–stasis syndrome : Compared with the model group,the ischemic myocardium , s MDH, 8 iron-sulfur protein, myosin light chain, glutathione peroxidase, Cu-Zn superoxide dismutase decreased in the model group,αB-crystallin protein,triosephosphate isomerase,protease subunit precursor increased .Conclusion:1 The model of myocardial ischemia (left anterior descending coronary artery ligation rat model )belongs to Qi-deficiency and Blood–stasis Syndrome2 The diffenret protein group of myocardial ischemia Qi-deficiency and Blood–stasis Syndrome are related with the proteins of energy metabolism, oxidative damage and myocardial self protection.The external manifestation of myocardial ischemia Qi-deficiency and Blood–stasis Syndrome is based on the changes of myocardial energy metabolism, oxidative damage, myocardial necrosis and myocardial fibrosis. |
PDF Full Text Request