Atractylodes Concocted Principle

The processing principle research for Atractylodes macrocephala Koidz. was Country-15 tackled key problem.which discussed the processing principle of baizhu, mainly through the aspects of chemistry, pharmacology and the dry property of trad. Ch. materia medica,etc. ,to provide the theoretical basis for making the quality standard of the processed sample .Main research contents:1 .Chemical researchThe extraction liquid for the raw and processed samples of baizhu was done the whole wavelength UV scanning to compare the absorption peak ,and under different absorption wavelengths we assay the samples before and after processing with HPLC The result of the contrasted altas analysis manifested that there were no new peaks, only changes occuring to the content.The content of atractrylone reduced,and which of Atractrylode I III increased.With the percolation and repeated silicagel column chromatography, atractrylone, Atractrylode I and III were massively separated to prepare for the pharmacological experiments.The stability of atractrylone was systematically studied. After the atractrylone crystal was laid under room temperature,its transformed products were determined by HPLC and GC-MS.on the basis of structure analysis and the contrast of similar compounds,we discussed its transformation mechanism. Under the room temperature , atractylone could be transformed into Atractylenolide, atractylenolide II and atractylenolide III; when heated, atractylenolideIIIcould be dehydrated , then turned into atractylenolide II. and we also studied the stability of atractrylone crystals under different conditions and its transformation rate into Atractrylode respectively.The contents of atractrylone Atractrylode I IIand III were determined before and after baizhu was processed.The chemical compounds of the processed baizhu were systemically separated, atractrylone, Atractrylode I . II, III, β -sitosterol ,biatractylenolide, β -amyrin acetate, taracetate were respectively recieved from the petroleum and ethyl acetate extract of baizhu.2.Pharmacological researchThe theory of tran. Ch .medicine thought: the eliminating dampness was weakened and reinforcing the spleen was strengthened after baizhu was stir-baked with wheat bran. Chemical research showed: in the processed baizhu,the content of atractrylone decreased ,meanwhile Atractrylode I III increased.atractrylone was extremely unstable and easily oxidized into Atractrylode.So the single compound of atractrylone, Atractrylode I and III were done the pharmacological experiment on the eliminating dampness and reinforcing the spleen. (1) dry property content researchWe designed to study the dry property of atractrylone through its effect on salivary gland secretion of rabbits and the water-intake of mice The result manifested that atractrylone could significantly inhibit the salivary gland secretion and increase the water-intake According to the theory of tra. Ch. medicine that domination of dryness brings about deficiency of fluid, we infered that atractrylone was possibly one of the dry property contents in baizhu, which provided the theoretical basis for clarifying the processing principle...