Metabolic Gene Expression Profiles. "syndrome - Genome" Methodology And Pedigree Cold Syndrome

BackgroundAfter the Human Genome Project to be completed, the arrival of post genome era provide an opportunity to study the Zheng of Traditional Chinese Medicine (TCM). At present, the important focuses of a research is to explore "Zheng-genomics", in which "Zheng-gene expression profile" confronts a great deal of puzzlements. Therefore, the methodology study on "Zheng-gene expression profile" has positive meanings to clarify therse problemes. It is not scientific attitudes that neither stick to established practices, nor underestimate oneself. We should explore a new way for the study of "Zheng-gene expression profile" in line with the spirit of seeking truth from fact.Objectiveâ‘  To establish methodology platform on "Zheng-genomics".â‘¡ To researtch gene expression profile on predigree in deficiency-cold Zheng.Methodsâ‘  Through the reviewing the up-dated references in the world on microarray technology systematically and data analysis, A theoretical fundamental for correct usage of microarray technology may be provided. A fuzzy analysis system on gene functions is set up by combining between traditional Chinese and western medicine from OMIM database. This system adopts the concept of integrity and regards the interaction between man and nature as guidance and refer to modern medicine pattern also. It provides an information platform for clinical application in gene expression related.â‘¡ According to the conception of different diseases may being same Zheng in medicine, the pedigree in genetics, the gene expression in molecular biology, the biologic statistics on mathematics and information mining of bioinformatics, we adopt 3 kind of experiment schemes to research one pedigree of deficiency-cold Zheng. A justment model of support vector machines is applied successfully for the Zheng by the result of indirect compareing. At the same time, we verificated the difference expression gene by the methods of RT-PCR and qPCR.Resultsâ‘  Through the analyzing the influence agents of experiment systematically, the advantages and shortcomings of the design and 21 kind of mathmatics methods were check out. A constructive suggestion is put forward for choosing an experiment scheme on "Zheng-gene expression profile".â‘¡ A fuzzy analysis system of combination between traditional Chinese and...