| Depression is a psychosis, which is characterized by various manifestations of affective disorder. It affects patients' working, learning and living so that they have difficulty adapting themselves to society , even induce suicidal behavior at last. With intense social competition and fast working pace, more and more people will feel tensive, exhausted and melancholy. Incidence rate of depression will show an upward tendency. Furthermore, relief of depression is related to the rehabilitation of body disease. Psychopathology of depression is not known. Treatment on depression that is based on the hypotheses has a variety of defects. Today, the research is focused on the pathogenesis of depression and the new drugs that are effective and have fewer side effects. On the basis of the traditional theories, traditional Chinese medicine (TCM) had played more and more important role in treating depression. Guided by TCM's theories, combining with contemporary theories, experimental and clinical results of TCM and Western medicine, developing new Chinese herbal preparation with good curative effects and fewer side effects is a new project. It will be of great importance to improve the effect of TCM on treating depression and understand the mechanism of treating depression of classical prescriptions. "Guanyu Capsule" is created to treat depression due to pathogenic fire derived from stagnation of liver-qi and phlegm-heat attacking the heart, accompanied by deficiency of the spleen. Our studies include prescription, pharmacy, action, toxicology and mechanism exploration. 1 The demonstration of Guanyu Capsule's prescription 1.1 Ingredients The chief herb: Herba Hyperici Perforati, adjuvant herb: Radix Curcumae, Rhizoma Acori Talarinowii, assistant herb: Radix Ginseng, Radix Polygalae, Flos Albiziae. 1.2 Action and indications Action: To disperse the depressed liver-energy, clear heat and eliminate phlegm, and tranquilize mind. Indications: Depressive disorders due to pathogenic fire derived from stagnation of liver-qi and phlegm-heat attacking the heart, with manifestation of deprementia, restlessness, tantrum, gas pain of chest and hypochondrium, palpitation, insomnia, morbid forgetfulness, dreaminess, festless sleep, painful abdominal mass, poor appetite, lassitude, red tongue, yellow and greasy fur, slide and rapid pulse. 2 Pharmaceutical research of Guanyu Capsule 2.1 production technology research of Guanyu Capsule Orthogonal design was used to optimize the production technology. The optimization technology: Rhizoma Acori Talarinowii was macerated for 2h and isolated volatile oil with 8 times volume of water for 15h, filtered, and decoction was reserved. The volatile oil was included by β-cyclodextrin. Radix Curcumae, Radix Polygalae, Flos Albiziae and Rhizoma Acori Talarinowii gruffs were macerated for 4h and extracted in sequence two times with 10 and 8 times volume of water for 1.5h respectively. The above decoctions were combined, concentrated to a thin extract with a relative density of about 1.04, added ethanol to a content of 60% ethanol, stored at 4℃ for 24h, filtered. The filtrate was recovered ethanol, concentrated and dried. Taking 60% ethanol as solvent, Herba Hyperici Perforati and Radix Ginseng were macerated for 2h and extracted with 10 times volume of ethanol for 1h, filtered, and the filtrate were recovered ethanol, concentrated and dried. To combine the above extraction and β-cyclodextrin inclusion compound, crush, mix well, pack into capsules. Preparation ofβ-cyclodextrin inclusion compound of volatile oil from Rhizoma Acori Talarinowii: To adopt the method of stirring, oil:β-CD was 1:4, and the inclusion temperature and time were at 30℃ and for 15 minutes. 2.2 Study on quality standards for Guanyu Capsule The content of Guanyu Capsule is dark brown powder, sour flavor and bitter. Weight variation, water determination, disintegration time, microbial limit were all in line with rule. Rhizoma Acori Talarinowii, Radix Ginseng and Radix Polygala were identified by TLC. The content of hy... |
PDF Full Text Request