The Chemical Composition Of The Three Kinds Of Medicinal Plants

This dissertation dealt with the chemistry of the natural resources of three folk medicinal plants, Laggera alata D. Don Sch.-Bip.ex.Oliv. (Compositae), Senecio tsoon-gianus Ling (Compositae) and Ligularia veitchiana (Hemsl.) Greenm. (Compositae). From above three plants, 60 compounds were isolated by using column chromatograghy and preparative thin layer chromatogrghy. 50 compounds structures of them were identified by spectroscopic, chemical and X-ray diffraction methods. This thesis comprises of four chapters.Chapter 1 Phytochemical Investigation of Laggera alataLaggera alata is mainly distributed in Africa and southwestern of Asia, especially in Yunnan Province of China. Its remarkable anti-inflammatory and antibacterial properties led to the naming of the plant in Chinese: "Chou Ling Dang". Previous investigation of the same species collected from Africa and the same genus plant Laggera pterodonta found in Yunnan Province had led to the isolation of eudesmane derivatives including some cytotoxic ones. These interesting findings have prompted us to a phytochemical examination of Laggera alata grown in Yunnaa 43 compounds were purified and 9 new compounds [( I -1~ I -8, I -20)], along with 29 known compounds of them were identified as1. 5β-Hydroxyilicic acid (I-1),2. 5β-Hydroxy-4-epi-ilicic acid methyl ester ( I -2),3. 3α-Hydroxyilicic acid ( I -3),4. 8α -Hydroxyilicic acid ( I -4),5. Alatoside A( I-5),6. Alatoside B( I -6),7. AlatosideC(I-7),8. Alatoside D ( I-8),9. Eudesma-3,5,1 l(13)-trien-12-oic acid (1-9),10. Iβ-Hydroxyilicic acid ( I -10),ABSTRACT11. Costic acid (I-11),12. Isocostic acid (I -12),13. ilicic acid ( I -13),14. 5ct-Hydroxy-p-costic acid (1-14),15. 5a-Hydroxycostic acid (I -15),16. 3-Oxoisocostic acid (I -16),17. 1 P-Hydroxycostic acid (I -17),18. 5p-Hydroxycostic acid (I -18),19. Eudesma-4(15),ll(13)-dien-12,5p-olide (I -19), 20.AlatosideE(I-20), 21.Tessaricacid(I-21), 22.-Sitosterol(I-22),23. Stigmasterol ( I -23),24. 3p,A4-stigmasten-3-ol (I -24),25. 3p-stigmasta-5,25-dien-3-ol (I -25),26. A4-stigmasten-3-one (I ?6),27. A4'22-stigmasten-3-one ( I -27),28. Artemitin(I-28),29. Chrysosptertin B (I -29),30. Luteolin-3,3',4'-trimethyl ether (I -30), 31.Casticin(I-31),32. Quercetin-3,3'-dimethyl ether ( I -32),33. Centaureidin (I -33),34. 3',4',5-Trihydroxy-3,7-dimethoxyflavone ( I -34),35. 5-Hydroxy-3,3',4',7-tetramethoxyflavone ( I -35),36. Quercetagein 3,3',6-trimethyl ether ( I -36),37. 2,5-Dihydrocinnamic acid ( I -37),38. 10-Methyl-tritriacontane (I - 38),The structure of compounds ( I -1), ( I -2) and (1-21) were confirmed by X-raydiffraction analysis. Compounds ( I -20) and (1-21) were first obtained as natural products. Some difference in eudesmane derivatives between Laggera alata and Laggera pterodonta was noticed. The cytotoxicity of selected constituents from Laggera alata was also investigated. The compound (I -29) showed the strongest cytotoxicity on KB cells of a inhibition ration 62% at concentration 10~5 mol/L among the selected samples.Chapter 2 Eremophilane derivatives from Senecio tsoongianousSeveral species of Senecioneae have been shown to be rich sources of sesquiterpene lactone and pyrrolizidine alkaloids, which exhibited anti-tumour activity. No chemical studies have been so far published on Senecio tsoongianous, a species specially distributed in middle and west of Yunnan Province. A chemical investigation of the Seneciotsoongianous led to the isolation of four new eremophilane lactone (II -1~ II -3 and II -5)apart from three known ones. The structures of known and new compounds were mainly elucidated by spectral data and shown to be:39. 2-Hydroxyl-14-nor-eremophila-l,3,5,n(13)-tetraen-6,12-olide(II-l),40. 3-Oxo-10p-hydroxyeremophila-7(ll),8-dien-8,12-olide (II-2),41. 1 p, 1 Op-Epoxy-8p-hydroxyeremophila-7( 11 )-en-8a, 12-olide (II -3),42. 8p-Hydroxy-eremophila-7(ll),9-dien-8,12-olide (H-4),43. 8a-Hydroxy-eremophila-7( 11 ),9-dien-...