Benefit Yangming Tianjin Gas To Delay The Beginning Of Old Female Rats The Ovarian Function Of Aging Mechanism

Objects: To explore the effect and mechanism of "tonifying Yang Ming Fluid and Qi" to delay the aging of the primary senile rat ovary and so that to testify the importance of TCM traditional classic theory "Yang Ming Meridian Declines during Wu Qi period" in premenopause. The study may offer a new study and differentiational thought to improve premenopausal women health, delay the aging of ovary founction, prevent and treat relative diseases. It also can offer pharmacodynamics evidence for manufacturing relative effective formulas.Methods: 4~6 months old SD female rats were set to be normal control group and 10~12 months old SD female rats whose oestrus elongation were set as primary senile female rat model. Primary senile female rats were devided into 5 groups randomly as following: I. High dose Yiweitang group; 2. Middle dose Yiweitang group; 3. Low dose Yiweitang group; 4. Diethylstilbestrol group; 5. Model control group. The intervention period was 4 weeks. The blood samples were tested by radio-immunity method to detect serum E2, P, T, FSH and LH. The ovaries were treated by HE and Immune histochemistry method to observe growing follicles, corpus luteum, vascular amount, structure of vascular across section, expression of Bcl-2, Bax, Fas, FasL, Caspase3, Cytochrome C. Ovary cells suspension was tested by Flow Cytometry (FCM) to detect apoptosis and the expression of right ovary Inhibin amRNA was measured by RT-PCR.Results: In modulating H-P-O-Ar aspect, compound Yiweitang which could tonify YangMing Fluid and Qi could increase uterus index, ovary index, adjust sex hormone level in blood, change the growth internal environment of follicle, promote the growth of follicle, vascular production of ovary, up regulate the expression of ovary Inhibin amRNA, ER and FSHR. In modulating ovary apoptosis aspect, Yiweitang could improve the expression of Bcl-2 in ovary and weaken the expression of Bax, which could increase the ratio of Bcl-2/Bax. It could also decrease the expression of Caspase3, Cytochrome C and reduce the percentage of apoptosis. Yiweitang could inhibit apoptosis from mitochondrial pathway, while couldn't reach the normal group level. Conclusions: The results showed that Yiweitang, which could tonify YangMing Fluid and Qi, could change the growth internal environment of follicle, promote the growth of follicle, increase the excretion of ovary inhibin and decrease FSH, improve the blood supply of ovary, increase the expression of hormone receptor and restrain ovary apoptosis. These effects may be the mechanism of Yiweitang delay the aging of primary senile rat ovary.