The Experimental Study About The Effect And Mechanism Of Gua Shi Tang To Ovarian Store Of The Primary Senile Female Rat

Objective:To discuss the effect and mechanism of Gua Shi Tang to improve ovary reserve of the primary senile rat ovary.The study may offer a new thought to improve ovary store founction, delay the age of ovary, improve premenopausal women health, prevent and treat relative diseases.Methods:4～6 months old SD female rats were set to be normal control group and 10～12 months old SD female rats whose oestrus elongation were set as primary senile female rat model. Primary senile female rats were devided into 5 groups randomly as following:Model group; High dose GuaShiTang group; Middle dose GuaShiTang group; Liuwei dihuang Wan group; Ginkui shenqi Wan group. After 4 weeks, weighed and sacrificed the rats by decapitation during anestrum underlying the result of vagina cell smear.Get blood by decapitation, centrifugalization, separating serum, placed them respectively, determined the level E2, P, IGF-1, FSH and VEGF by means of radio immunoassay and enzyme-linked immunosorbent assay, dissected and got rats ovary, uterus, spleen and paranephros quikly,rejected the surrounding tissues,slightly wiped filter paper,weighed and calculated the ponderal index. Fixed ovary and uterus in 4% polyoxymethylene, dehydrated normally, soaked with wax,embedding,slice in 4μm,HE dyeing,observed the constructure of uterus and ovarian histomorphology under light microscope. The ovaries were treated by immune histochemistry method to observe the expression of INHB, ER, FSHR, Bcl-2 and Caspase-3Results:The contents of serum:Compared with normal control group,the contents of serum E2 and P in model group are obviously decreased (P<0.05).Treated with GuaShiTang, the contents of serum E2 and P raised obviously in middle dose and high dose group,compared with model group,there are statistical significance (P<0.05); Liuwei dihuang Wan group and Ginkuishenqi Wan group could increase the contents of serum E2 and P, while compared with model group, there are no obviously statistical significance (P>0.05); GuaShiTang, Liu wei dihuang Wan and Ginkuishenqi Wan had no obvious influence to FSH. GuaShiTang, Liuweidihuang Wan and Ginkuishenqi Wan could increase the ponderal index of ovary, uterus, spleen and paranephros, raise the contents of serum VEGF and IGF-1 obviously, as compared with model group, there are significant difference (P<0.05).Histomorphology changes in ovary of rats:the appearance of ovary is normal,grades of follicles and corepus luteums can be seen on it in normal control group.While in model group,there can be seen very less grades of follicles, mature follicles and corepus luteums are less,the caliber of vessels become thinner, compared with normal control group,there are statistical significance (P<0.05).There can be seen grades of follicles on it in the middle dose and high dose groups of GuaShiTang, Liuweidihuang Wan group and Ginkuishenqi Wan group, the blood vessel in the ovary is normal basically,grades of follicles can be seen on these groups, compared with model group,there are statistical significance (P<0.05). Compared with model group, the hilus vessel wall in other groups'rats became thinner and its cavity expanded to increase blood supply to the ovary.The expression of Bcl-2, Caspase-3:Compared with normal control group? the model group has a very significant difference (P<0.01); As compared with the model group, Liuweidihuang Wan group, Ginkui shenqi Wan group and the high dose GuaShiTang group have significant difference (P<0.05). The expression of INHB:compared with normal control group, the model group has a very significant difference (P<0.01); as compared with the model group, Liuweidihuang Wan group and the high dose GuaShiTang group have significant difference (P<0.05). Compared with the model group, GuaShiTang could increase the expression of ERβ和FSHR and there are statistical significance (P<0.05), but not to the degree of the normal control group.Conclusion:①GuashiTang and nourishing kidney herbs might have the effect of improving ovaries function and raising the contents of serum E2 and P.②poptosis regulator Bcl-2,Caspase-3 protein and INHB are closely related with the ovarian reserve. The inhibition of ovary apoptosis and the reduction of follicular atresia are potential mechanism of improvement of ovary reservation.③Gua Shi Tang can delay ovary aging possibly by regulating vasomotion and vascularization to improve blood supply to the ovary in order to improve the function of the ovary. These might be the mechanisms of GuaShiTang delaying the aging of ovary in primary senile rat...