Experimental Studies On The Treatment Of Hypothermia Induced By Adenosine 5'-Monophosphate On Gouty

Objectives:To investigate the effect of the hypothermia induced by Adenosine 5'-Monophosphate (5'-AMP) on gouty arthritis.Methods:Ankle joint injection with monosodium urate monohydrate (MSU) crystals in hypothermia rats model which was induced by 5'-AMP, and then observe the effect of hypothermia induced by 5'-AMP on acute gouty arthritis via detecting the swelling of rat ankle, serum level of Interleukin-1β(IL-1β) and metalloproteinase-9 (MMP-9), the synovium tissue of rat ankle and the Nuclear Factor-KappaB (NF-κB) activity.Results:The hypothermia induced by 5'-AMP has protective effects on acute gouty arthritis, which was demonstrated by the following criteria:1) a significant reduction of the ankle swelling (p<0.001); 2) a significant decrease in the occurrence of leukocyte infiltration and mild hemorrhage; and 3) a significant reduction in the presence of IL-1β(p<0.001) and MMP-9 (p<0.001).4) a significant inhibition in the Nuclear Factor-κappaB (NF-κB) activity (p<0.001).Conclusions:Hypothermia induced by 5'-AMP could inhibit acute inflammation reaction and protect the synovial tissue against acute injury in a rat acute gouty arthritis model.