Effects Of Haoqingqindan Decoction On Aquaporin In Damp-heat Syndrome Of Mouse By Influenza Virus Infection

Viral infections of respirovirus are common and frequently occurring diseases, in which the influenza was most severe and broke out actively recent years. Viral diseases clinical manifestations of damp-heat syndrome characteristics, especially in the south of the Five Ridges area because of the climate and geographical environment, eating habits and other effects, viral infection of the respiratory tract are more obvious. Damp-heat syndrom and fluid metabolism are closely related, and aquaporin (AQP) is the important material of body's regulation of water metabolism, so the damp-heat syndrome and aquaporin relationship also caused the extensive concern of scholars. At the same time, according to Chinese medicine theory, damp-heat pathogen diffuse the whole organs, cause body fluid metabolism disfunction, therefore to observe the aquaporin changes, we should observe all water metabolism related organs changes. Preliminary experimental and clinical practice showed Haoqin Qingdan Decoction in treating virus infection of respirovirus infection in south of the Five Ridges of damp-heat syndrome have a significant effect, through observe the effects of damp-heat factor on aquaporin in damp-heat syndrome of mouse by influenza virus infection, and Haoqin Qingdan Decoction and its disassembled prescription to this intervention effect, to discuss the material foundation of damp-heat in body fluid metabolism disfunction and Haoqin Qingdan Decoction in regulating the metabolism of body fluid.Object ive:We use heat and humid environment+richly fatty and sweet diet+pathogenic factor to establish the animal model of influenza virus infect mouse with damp-heat syndrome. Observe endogenous and exogenous damp-heal factors'effects on the expressions of influenza virus in lung, AQP1,AQP5in lung, AQP4in stomach, AQP1in colon and AQP2in kidney in mice, to discuss the material foundation of damp-heat in body fluid metabolism disfunction. At the same time, Observe Haoqin Qingdan Decoction and its disassembled prescription effects on the expressions of influenza virus in lung, AQP1,AQP5in lung, AQP4in stomach, AQP1in colon and AQP2in kidney in mice, to discuss the treatment mechanisms of Haoqin Qingdan Decoction in viral diseases in damp-heat syndrome by eliminating dampness.Methods1. To study the effect of exo-endo damp-heat factors on AQPs in different organ of mouse with damp-heat syndrome and influenza virus infection:50mice of BALB/c were devided into4group randomly (control group, damp-heat group, model group, viral group), then observed the symptom and sign of mice. After executions, observe lung index and the expression of Influenza virus in lung, AQP1,AQP5in lung, AQP4in stomach, AQP1in colon and AQP2in kidney in mice by immunohistochemical method and FQ-PCR.2. To study the effect of Haoqin Qingdan Decoction and its disassembled prescription on AQPs in different organ of mouse with damp-heat syndrome and influenza virus infection:115mice of BALB/c were devided into8group randomly (control group, model group, Ribavirin group, HQQD group, decomposed1group, decomposed2group, decomposed3group, decomposed4group), then observed the symptom and sign of mice. After executions, observe lung index and the expression of Influenza virus in lung, AQP1,AQP5in lung, AQP4in stomach, AQP1in colon and AQP2in kidney in mice by immunohistochemical method and FQ-PCR.Results:1. The mortality of model group was a little higher than viral group. The lung index of model group is a little lower than viral group, but the difference wasn't significant (P>0.05).2. The expression of AQP1,AQP5in lung, AQP4in stomach, AQP1in colon and AQP2in kidney in model group shows significant difference compaired with control group. The expression of AQPs shows no signi ficant difference between damp-heat group and control group (P>0.05). The expression of AQPs shows significant difference between damp-heat group and control group (P<0.01 或P<0.05). Viral group cmopaired with control group, the expression of AQP1in lung, AQP4in stomach, AQP1in colon and AQP2in kidney shows no significant difference (P>0.05), and the expression of AQP5in lung shows significant difference (P<0.05). Model group cmopaired with Viral group, the expression of AQPland AQP5in lung shows no significant difference (P>0.05), the expression of AQP4in stomach, AQP1in colon and AQP2in kidney shows significant difference (P<0.01).3. The expression of influenza virus mRNA in HQQD group, Ribavirin group and decomposed3group shows significant difference compaired with Model group (P<0.01or P<0.05)4. HQQD group, decomposed3group and decomposed4group compaired with Model group, the expression of AQP1,AQP5in lung, AQP4in stomach, AQP1in colon and AQP2in kidney in model group shows significant difference (P<0.01or P<0.05). Decomposed1group compaired with Model group, the expression of AQP4in stomach, AQP1in colon and AQP2in kidney shows significant difference (P<0.01or P<0.05). Decomposed2group compaired with Model group, the expression of AQP4in stomach and AQP2in kidney shows significant difference (P<0.01or P<0.05)5. HQQD group compaired with decomposed3group and decomposed4group, the expression of AQP1,AQP5in lung, AQP4in stomach, AQP1in colon and AQP2in kidney shows no significant difference (P>0.05). HQQD group compaired with decomposed1group, the expression of AQP1,AQP5in lung, AQP4in stomach shows significant difference (P<0.01or P<0.05). HQQD group compaired with decomposed2group, the expression of AQP1in lung, AQP4in stomach, AQP1in colon and AQP2in kidney shows significant difference (P<0.01or P<0.05)Conelusions:1. Model group compaired with viral group, the expression of influenza virus shows no significant difference, it means that exo-endo damp-heat factors can not influence the copy of influenza virus.2. The joint action of exo-endo damp-heat factors and influenza virus infection can cause body fluid metabolism disfunction, but the single factor exo-endo damp-heat or influenza virus infection shows no such effect.3. The patho]ogical organ of model group is lung, but the expression of AQPs in many organ are abnormal, this result fits the theory of damp-heat diffuse triple energizer in TCM. This result indicates that abnormal expression of AQPs may be one of the important, material foundations of damp-heat syndrome. It also indicates that we should study the relationship between AQPs and damp-heat syndrome through the integral view.4. Haoqin Qingdan Decoction can inhibite the copy of influenza virus in influenza virus infect mouse with damp-heat syndrome. Zhuru, Banxia, Puling, Huashi, Gancao in Haoqin Qingdan Decoction can inhibite the copy of influenza virus, we assume the reason is that this kind of herb can regulate the disfunction of body fluid metabolism, and coordinate the function of Qinghao, Huangqin, Daqingye, Banlangen.5. Haoqin Qingdan Decoction can regulate the expression of AQP1,AQP5in lung, AQP4in stomach, AQP1in colon and AQP2in kidney of influenza virus infect mouse with damp-heat syndrome. This may be the material foundation of eliminating dampness caused by Haoqin Qingdan Decoction, and the effect influence the whole bady.6. Qinghao, Huangqin, Zhuru, Banxia, Fuling, Huashi, Gancao in Haoqin Qingdan Decoction show important effect to regulate the disfunction of body fluid metabolism, Chenpi, Zhike, Daqingye, Banlangen in Haoqin Qingdan Decoction show no such effect.