| ObjectiveIn this study the classical theory of Chinese medicine is based on the use of temperature,cold air under the actual situation of drug production in the rat model,based on the combination of environment,diet and other factors,the production of dominant damp over heat and dominant heat over damp in the rat model,Qingre Huashi recipe with addition and subtraction of the two models, respectively,to intervene in rats were observed before and after treatment of rats with body temperature,food intake,water intake, the second will,spirit,activity,color changes and other macroeconomic indicators,The use of radioimmunoassay and immunohistochemical detection of rat tumor necrosis factor-α(TNF-α),IL-1β(IL-1β),nuclear factorκB(NF-κB),were analyzed in order to investigate the evidence of heat some properties and dampness over heat and heat re-wet model in the method of in-depth study and experiments provide a basis for clinical practice. MethodsMulti-factor "Chinese medicine garage+high-fat diet+ high-temperature and humid environment" composite model method model damp-heat syndrome.The establishment of the normal control group(A),wet weight in the thermal model group(B),wet weight in the heat treatment group(C),wet heat than the model group(D) focus on the wet heat treatment group(E)①A:Under normal temperature and humidity environment,the ordinary mixed diet 29 days.②B,C,D,E:in the normal temperature and humidity environment,with high-fat diet for two weeks,during which the B, C group of the first week garage Dachengqitang small(each administered orally 2g/ml/200g,the second day),D,Egroup fed with the first week of rhubarb breviscapus Aconite Decoction(oral administration of each 2g/ml/200g.the second day),after two weeks, then put me in Phytotron 12 days(B,C temperature 25℃,relative humidity 95%;D,E temperature of 35℃,relative humidity 75%), the daily model time(the morning of 8-12 pm,13-17 pm),12 days after the were fed with E,coli 109/ml/200g.4 hours after garage once again stepped up their efforts to continue to observe the three days.③C,E:in the first E.coli after garage,garage decoction of Chinese medicine for the corresponding evidence to the contrary. To observe the rat's body temperature,mental state,activity, water intake,food intake,and the two will change color and so on. The 30th day rats were killed in the electron microscopy of rat liver tissue biopsy,using radioimmunoassay and immunohistochemical detection of rat serum and liver tissue of TNF-α,IL-1β,NF-κB level.SPSS13.0 data package are one-way ANOVA,the results to mean±standard deviation((?)±s) indicated,P＜0.05,P＜0.01 indicated statistical significance.ResultsNormal rats had no significant symptoms and signs of change, partial partial wet hot model group rats increased body temperature, weight loss,water intake and food intake than normal decrease in fatigue prone Anopheles,yellow turbid urine,thin soft stool, Matte color dark yellow,hot side temperature of the model group than the high side wet model group.Hot wet treatment of migraine migraine symptoms in rats,signs of the model group than those of light,and fast restore,partial restore hot treatment group than the treatment of migraine-type group quickly.The model group,serum and liver tissue TNF-α,IL-1β,NF-κB was significantly increased compared with the normal group were significantly different(P＜0.05 or P＜0.01),comparison between the two models are significant difference(P＜0.05).Treatment of rats TNF-α,IL-1β,NF-κB level than the model group significantly lower(P＜0.05),compared with the normal group no significant differences between the two treatment groups showed no significant difference(P＞0.05).Electron microscopy of liver biopsy:group liver tissue two models are damaged,liver cells in the central belt of fat change,portal inflammatory cell infiltration area shows inflammatory reaction heat model.Thermal model biased towards the model group than in the wet change of fat liver cells,inflammatory cells and more.The two treatment groups narrowed liver tissue damaged,some animals with intact peripheral liver tissue cell cytoplasm darker colored,more Alkalophilic quality,no significant difference between the treatment group. ConclusionThe adoption of the model group rats under cold temperature or interfere with the formation of two different drugs of the actual situation in the gas model of E.coli infection,the two rats of model group were increased body temperature and body temperature of heat towards the model group than the wet high emphasis on the model group,after the intervention by Chinese medicine,hotter over damp more emphasis on the treatment group than the treatment group to restore the body temperature of fast notes to actual situation on the dampness over heat of gas in the heat and hot models focus on the formation of Damp-heat syndrome impact,the two models in rats are the basic signs and symptoms disease in line with the temperature in the characteristics of evidence.Model group TNF-α,IL-1β,NF-κB levels compared with the normal group has a very significant difference,and its pathophysiological effects,from the immunological aspects of a good interpretation of the incidence of heat illness laws of evidence and clinical performance.Which TNF-α,IL-1β,NF-κB levels in the two comparison between the model group were significantly different on the two model damp-heat syndrome group,wet weight and heat in the heat of the different emphasis on the Damp-heat syndromeQingre Huashi recipe addition and subtraction can improve the damp-heat syndrome rat model of signs and symptoms,and the two immune parameters in model group and the nuclear factor NF-κB back to normal on Qingre Huashi side model addition and subtraction of the two intervention groups have better inhibit the transcription of inflammatory mediators to suspend,limit the acute inflammatory response,with better results Qingre Qushi. In short,the model from the above signs,indicators,and Qingre Huashi pathological side interventions,such as addition and subtraction,it may be some evidence that there is hot and damp dominant in the Damp-heat syndrome had different dominant part, different aspects of that heat from the wet weight of evidence in heat and heat re-create the success of the Damp-heat syndrome model.
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