The Research Of Mechanism About Lung Injury By Exogenous Dryness

Exogenous Dryness is one of the unique concept of traditional chinese medicine (TCM),It also one of the six exopathogens, was first recorded in NEIJING. also discussed by later physicians, or list its like, or cause of the sex, or shown in the square, all kinds of records and scattered among some theoretical writings. Exogenous Dryness leads to lots of diseases, but its characteristics less than wind, cold, heat, summer heat and other exopathogens,farthermore, Exogenous Dryness sometimes was confused with wind, cold, heat, summer heat and other exopathogens,it was found have both warm and cold performance in clinical, so the research of exogenous dryness could not keep abreast of with the other exopathogens. On the base of previous studies,this study maked a research with the concept of exogenous dryness,the considerations of ancient experts with exogenous dryness,the seasonal of exogenous dryness,the regional of exogenous dryness,the Property of Yin and Yang with exogenous dryness,the natural characteristic of exogenous dryness,the Pathogenic characteristics of exogenous dryness,the Law of mass change of exogenous dryness,The theory of the lungs Weather medical research. And On the basis of the experimental study, based on the natural characteristics of dry outside, to build a simulation model under natural conditions exogenous dryness, project to pulmonary surfactant mice DPPC, SP-A, SP-D, bronchial and lung epithelial cell nuclear factor NF-κB, lung mucus gene MUC5AC impact as the contact point, delve into the Mechanism about Lung Injury, Found the higher level Experimental evidence of the Mechanism about Lung Injury, Then play a positive role in the research of six exopathogens and the clinical traditional Chinese medicine. Theory researchAccess to relevant information, summarizing the characteristics of exogenous dryness. Chinese medicine " dryness " concept, is the " dryness " the phenomenon of long-term understanding and observation, based on image-based thinking get analog formed. It is difficult to make precise, single definition, it should be from the etiology, pathogenesis, symptoms and other different aspects of the analysis and understanding. Ancient physicians will be a natural phenomenon of air-drying after the onset of symptoms and characteristics of the human body analogy, who summed up and dry climate-related, with drying Kuse, damage to body fluid, easy to violations of the characteristics of lung and other risk factors, is the " drypathogen " " Dryness " is one of the six natural climates, and six climates is a natural yin and yang result of changes in movement. Produced outside the dry and year, season, geographic and other closely related. According to theory of Five and Six, Kim Yun too much of the year " drypathogen gas pop", prone to excessive dry air occurs, the situation changes too fast, resulting in the case of dry diseases; timber transport operation is less than less than a year of wood, metal, wood, and the " drypathogen is full of the air" dry diseases of the situation is more likely to occur. With the development of social productive forces, generated exogenous dryness content of a new, man-made "exogenous dryness" is increasing. Penetration of air conditioning in modern society more and more people enjoying the cool, at the same time, more prone to a little "exogenous dryness " environment. Mainly in autumn, Four Seasons all; in Place it shows West higher, and the exogenous dryness was found in every directions. There is a lot of controversy with the Property of Yin and Yang with exogenous dryness, partakers yin, yang, and with the both views, exogenous dryness, there are five main pathogenic characteristics:lesions in the lung-based, easily hurt the body fluid, easy from the cremation, easy cutting grams of liver, hinders the qi activity, exogenous dryness intrusion from the outside into the human body to follow, from top to bottom, from the mass changes and blood gas law, according to the nature of the cold temperature, respectively, have different symptoms. "NEIJIN" explain the general principles point of "moisten the dryness" and the treatment "treat with Kuwen, Sasuke with Ganxin, purgation with Kun", ancestry doctors on this basis tapped a lots, inside dryness and exogenous dryness the two are mutually different, but also closely linked concepts. Differences are:the concept of property different syndromes of a different nature, different clinical manifestations; contact mainly two:in the pathogenesis, clinical manifestations are related.This research subjects of the collection, collation, analysis of ancient Chinese literature, "exogenous dryness" the relevant content, the concept of external dry, seasonal, regional, Yin and Yang properties, pathogenic characteristics, mass change rule, the natural characteristics and production, therapy summarized, compared to complete system of Chinese medicine " exogenous dryness" theory. Experimental studymethodsEach in man-made climate cabinet and mice, using "reference literature temperature-humidity-wind" comprehensive stimulation method to establish the model, by artificially climate box experimental conditions are controlled accurately. Each in the first 6,12, respectively,18 days after handling animals broken neck, specimens-80℃refrigerator frozen. Set aside.1. Lung tissue MUC5AC gene expressionUsing real-time fluorescence quantitative polymerase chain reaction (PCR) method to detect.2. Lung tissue NF-κB gene expressionUsing real-time fluorescence quantitative polymerase chain reaction (PCR) method to detect.3. Bronchial NF-κB activityAdopt protein immune imprinting (Western blotting) method to detect4. Alveolar lavage appear SP-A contentBy using the quantitative ELISA test5. Alveolar lavage appear SP-D contentBy using the quantitative ELISA test6. Alveolar lavage appear DPPC contentUsing HPLC method detection.The results1. MUC5AC gene expression. Normal temperature often wet when groups of gene expression MUC5AC significantly below other group (P< 0.01), temperature cool dry dry group, the first 12 days, the first of 18 days than their MUC5AC gene expression for the first six days express (P< 0.01), and normal day 18 of the dry MUC5AC of gene expression below its first 12 days express level, higher than the sixth day level.2. NF-κB gene expression. Wen dry and cool dry group phase NF-κB gene expression significantly lower than normal temperature often dry group (P< 0.01), temperature phase dry groupsκB gene expression-NF compared with normal temperature reduced dry group (P< 0.05), dry first 12 days, the first of 18 days above normal gene expression for the first six days, dry group expressed significantly higher (P< 0.01). 3. NF-κB activity. Cool the first six days, dry group for the first 12 days, the first 18 days lung tissue NF-κB activities were lower than normal temperature often wet group (P< 0.01), the first 12 days of normal, dry day 18 of the NF-κB activity also below the normal group (P< 0.01). Wen dry group, cool dry group day six NF-were lower than normalκB active dry, while the second group 12 days, the first 18 days, no obvious difference.4. SP-A content. Wen dry group and cool dry in the first six days of the first 12 days, first,18 days SP-A content were higher than normal temperature often wet group (P< 0.01), and normal phase dry groups SP-A content were significantly higher than normal temperature often wet group (P < 0.01). Wen with cool dry dry group SP-A content of the first six days, the first 12 days no difference.5. SP-D content. Wen dry group and cool dry in the first six days of the first 12 days, first,18 days SP-D levels are higher than normal group (P< 0.01), temperature cool dry dry group, the sixth day, the first 12 days SP-D levels higher than normal dry group (P< 0.05), wen day 18 of the dry group at SP-D levels higher than dry group (P< 0.05), and cool dry group content and no difference under dry group.6. DPPC content. Wen dry group, DPPC content and cool dry group compared significantly reduce the normal group (P< 0.01), wen dry, cool dry without difference compared the two groups. The first six days of temperature dry, cool dry on the sixth day, first of DPPC content at 18 days than dry group (P< 0.05), wen dry group 12 days, the first 18 days cool dry DPPC content for the first 12 days of significantly higher than normal dry group (P< 0.01), cool dry group day 12, the first 18 days of cool dry DPPC content for the first 12 days of significantly higher than normal dry group (P< 0.01),conclusions1. different categories of the infringing mice, dry evil MUC5AC gene expression of mice were raised, and there exists degree of difference. Explain the different categories of infringing mice after dry evil, lungs, pulmonary cannot ramrod waterway fluid retention become phlegm retention.2. each made mold conditions, each made timely phase, mould condition promptly phase of bronchial NF-κB in mice, the degree of gene expression cut increases over time, suggesting the extended dry lung, damage vital qi pullbacks injury.3. outside dry evil invade mice led to the lungs and reduceκB activity-NF, immune function, including temperature drop to dry and cool dryκB activity of NF-no differences influence.4. outside dryⅡstruck lung, mice alveolar type cells and airway Clara cells increased SP-A synthesis and secretion, used to resist the evil influence outside to dry damage the lungs.5. in different temperature humidity space under the condition of dry model, mice SP-stimulate the synthesis and secretion of D increases, tip the body after dry, lung injury against the pericardium inspired upright.6. outside dry model condition, temperature, dry group DPPC content and cool dry group less than the normal group, tip the dry evil fluid, and mice lung injury affecting its main gas, company breathing function.