The Study The Mechanism And Effect Of Yisuijiannao Capsule On Alzheimer Disease Rat

Purpose:The present study discussed the mechanism of the treatment of Yi Sui Jian Nao Capsule to Alzheimer's (AD).Method:The study demonstrates the validity of Yi Sui Jian Nao Capsule treating AD throμgh the water maze experiment and step throμgh test by determining the latency of water maze experiment and step throμgh and performing the brain histopathological examination after establishing the animal models of AD throμgh duplicating Aβinjection hippocampus of mice. On the basis of the cholinergic hypothesis theory of AD, the AchE, BchE, MDA, SOD, CAT, GSH-Px, MAO were also determined by applying immunohistochemical method and chemical colorimetric method and RT-PCR.The results:1.After oral administration of Yi Sui Jian Nao Capsule to AD mice for 15 days, the brain histopathological examination indicates that the AD mice after oral administration are better than AD mice. The latency of water maze experiment of the oral administration AD mice. The differences were considered statistically significant.2.After oral administration of Yi Sui Jian Nao Capsule to AD mice for 15 days.the content of MDA and the activity of MAO of oral administration AD mice's brain tissue decreased compared with AD mice, but the activity of SOD, CAT,GSH-Px increased. The differences were considered statistically significant.3.After oral administration of Yi Sui Jian Nao Capsule to AD mice for 15 days.the activity of AchE and BchE of the oral administration AD mice's brain tissue is lower than that of AD mice. The differences were considered statistically significant.4.After oral administration of Yi Sui Jian Nao Capsule to AD rats for 15 days,the number of oral administration AD rats'brain tissue apoptosis cells is lower than that of AD rats. The differences were considered statistically significant. 5.After oral administration of Yi Sui Jian Nao Capsule to AD rats for 15 days, the expression of Fas, Fas-L of the treatment AD rats'brain tissue is lower than that of AD rats. The differences were considered statistically significant.6.After oral administration of Yi Sui Jian Nao Capsule to AD rats for 15 days, the expression of Bcl-2 of the treatment AD rats' brain tissue increased compared with AD rats, while the expression of Bax decreased. The differences were considered statistically significant.Summary:1.In conclusion, the results of experiment indicate that the Yi Sui Jian Nao Capsule could shorten the water maze experiment latercy of mice. And the drμg could prolong the step throμgh validate the effect of Yi Sui Jian Nao Capsule to AD.2.The study shown that the Yi Sui Jian Nao Capsule could decrease the MDA production and the activity of MAO, but increase the activity of SOD, CAT, GSH-Px. They verify that antioxidation injury is one of the mechanisms of AD treatment by Yi Sui Jian Nao Capsule.3.The present study shown that the Yi Sui Jian Nao Capsule could reduce the activity of AchE and BchE of the brain tissue. It verifies that modulation the central cholinergic transmission is one of the mechanisms of the Yi Sui Jian Nao Capsule to AD.4.The present study shown that the Yi Sui Jian Nao Capsule could upregulate the expression of bcl-2 of brain tissue, while down-regulate the expression of Bax, Fas-L, Fas. And reduce the number of brain tissue apoptosis cells. It is one of the mechanisms of the effect of Yi Sui Jian Nao Capsule to AD.