| ObjectiveInfluenza is a common frequent acute respiratory tract infectious disease caused by Influenzal Virus (â…£) which rages in some places even around the world. There were five big rages worldwide in the 20th century that destroyed seriously. Losses caused by flu deserve anything but ignoring. According to the report of American National Science and Technology Council, recently about 20,000 to 40,000 people die of flu annually in U. S. Influenza has been a viral infectious disease with high mortality rate. Expenses for preventing flu and labor loss due to flu amount to over 120 million dollars each year. Flu is still an infectious disease, formidably threatening to human health and life.There is great advance in the anti-virus study in medicine and vaccine in the field of western medicine. However, for the continuous variation of antigen of bird-flu, the limitations and the side effect of the anti-virus medicine and vaccine in some degree, the perfect method to prevent and cure flu has not come out yet. The research for controlling flu has a long way to go.Flu belongs to "epidemic cold" in traditional Chinese medicine (TCM) theory, one of exogenous febrile diseases. So far the doctors of TCM have accumulated rich experiences in prevention and curing for eternal febrile diseases by different ways with advantages. Then the paper studied the way of treating influenza in basis of unification of "Differentiation of Six Channels" and "Differentiation of Four Systems(wei, qi, ying, and xue)" and "Differentiation of Tri-energizer".Then in order to study the mechanism and pharmacology of cold-warm combinational compound recipe Yinqiaochaigui granules treating influenza, some experiments are did.Experimental research were done for investigating the mechanism and evaluating the security and effectiveness of Yinqiaochaigui granules on regulation of the immune, protection of the organs and inhibition of the virus. Clinical research were done by observing the effect, the improvement of symptom scores, the curve of temperature drops, blood cells to clear about Yinqiaochaigui granules' practical, security and effectiveness. So it will provide the experimental evidence for the clinical application of Yinqiaochaigui granules.Experimental Research MethodsTo study the mechanism of Yinqiaochaigui granules to cure influenza, we did some experiments. This experiments takes mouse infected by influenza virus dropping nose as the model and uses Yinqiaochaigui granules to carry out experiments. The NIH into the mice is randomly divided into nine groups:normal control group,model control group, Amantadine group, the group on high dose of traditional Chinese medicine, the group on medium dose of traditional Chinese medicine, the group on low dose of traditional Chinese medicine.There are 10 mice in each group. Under slight anesthesia caused by ether, the mice are infected by dropping influenza virus into the nose. After the day the virus attacks, medicine is given. Then, according to the objective of every expeiment, relevant indexes are measured. The experiments included:1. Acute toxicity of Yinqiaochaigui granules;2.The pharmacodynamics study including the lung exponent and life protection;3. Study of Yinqiaochaigui granules' influence on the phagocytizing function of IV FMi infected mice's macrophages.Result(1) Acute toxicity of Yinqiaochaigui granules:There is no mice died. The traditional Chinese medicine low/medium/high dose have no toxicity.(2)Study of Yinqiaochaigui granules'influence on the inhibition of the IV FM1 infected mice's pneumonia:compared with normal control group,the lung exponent of the model group increase obviously which have significant difference(P<0.05). Compared with the model group,the lung exponent of amantadine group and traditional Chinese medicine low/medium/high dose group all reduced which have significant difference(P<0.05). Besides, the correlation of lung exponent and the medicine dose are negative, inhibition rate of lung and the medicine are positive.(3) Study of Yinqiaochaigui granules, influence on the protection of theâ…£FM1 infected mice, s death:Compared with model group, the average life time of the FM1 infected mice in traditional Chinese medicine of high/medium/low dose groups all improved obviously;The life protection rate of the FM1 infected mice in traditional Chinese medicine of high/medium/low dose groups were 35%,25%,15%. The health extension rate of the FM1 infected mice in traditional Chinese medicine of high/medium/low dose groups were 38.06%, 32.26%,25.18%. The life protection rate and the health extension rate of the FM1 infected mice in amantadine group were 60%,43.31%.(4) Study of Yinqiaochaigui granules'influence on the phagocytizing function ofâ…£FM1 infected mice's macrophages:Compared with normal control group, the OD(optical delnsity) of model group was lower (P<0.05) which indicate the phagocytizing function ofâ…£FM1 infected mice's macrophages reduced obviously;Compared with model group, the OD of medication groups were higher(P<0.01).ConclusionSummary, Yinqiaochaigui granules can alleviate the pathological changes in the lung of FM1 infected mice and have a protective and life extension effect on FM1 infected mice. Meanwhile, Yinqiaochaigui granules can improve the phagocytizing function ofâ…£infected mice's macrophages so that have the regulative funcation of the immune.Clinical Research MethodsThis research uses the forward-looking clinical stochastic comparison plan. The selected cases collected from the Guangzhou University of Traditional Chinese Medicine First Affiliated Hospital of outpatient and emergency patients, were randomly divided into the traditional Chinese medicine high dose group, the traditional Chinese medicine conventional dose group and the pure western medicine group three groups, integrates the case number is 142 examples. The symptoms, signs, the improvement of symptom scores, the curve of temperature drops of patients were observed in the research. Result(1)Compared with the western medicine group,the changing rate of temperature in different time of Yinqiaochaigui granules' groups were higher(P<0.01). And The traditional Chinese medicine conventional dose group and traditional Chinese medicine high dose group were more significant statistic difference (P<0.01). Comparing the curve of temperature drops of the three groups with test of sphericity, the statistic difference were all significant(P<0.05).The result indicate that the effect of defervescence of Yinqiaochaigui granules was superior to western group, high dose Yinqiaoachaigui granules was more effective.(2) The improvement rate of symptom scores of Yinqiaochaigui granules groups were higher than western group(P<0.01). But the difference between the improvement rate of symptom scores of Yinqiaochaigui granules high dose group and conventional dose group was not significant(P>0.05). Compare with the reduction of integration of flu syndromes, the Yinqiaochaigui groups were superior to the western one. There was not dose-effect relationship between the two groups of Yinqiaochaigui.(3)The WBC and Neutrophils of Yinqiaochaigui granules goups were reduced after treatment. But the change of WBC and neutrophils was in the normal range. The lymphocytes have increasing tendency after the treatment of Yinqiaochaigui granules groups, the difference was significant (P<0.01).(4)The therapeutic effect, TCM syndromes effect of Yinqiaochaigui granules groups were superior to the western group, the difference was significant (P<0.05). The integrated effect of Yinqiaochaigui granules was obviously superior to the western medicine.ConclusionYinqiaochaigui granules have the significant clinical effect, especially in the fact of defervescence and alleviating flu symptoms. And the immune regulative funcation of Yinqiaochaigui granules was confirmed.
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