The Study On Mitochondria Activity Of Muscles-Bones In Ovariectomized Rats And The Intervention Of GuKang

Objective:Analysis the mitochondria activity of muscles-bones in ovariectomized rats and the intervention of GuKang, which to investigate the relationship between the change of qualitative and quantitative of muscle-bone and the formation mechanism of osteoporosis.Method:48 female non-pregnant SD rats(6 months) were randomly divided into 4 groups: normal group(n=12), ovariectomized group(n=12), control group(n=12) and observation group(n=12). After establishing model, the rats of observation group were given the intragastric administration with GuKang. The rats of control group were given estradiol valerate, other groups were given distilled water. After treatment for 12 weeks, the bone mineral density (BMD) and the content of super oxide dismutase(SOD), malondialdehyde(MDA) were detected, mitochondrion were extracted from the muscle tissue that was collected during the operation, and cytochrome c(Cyt C), the mitochondrial permeability transition pore(MPPT) and Succinatedehydrogenase (SDH) were measured. 4 New Zealand rabbits(3 months) were randomly divided into control group(n=2) and observation group(n=2). The observation group was given the intragastric administration with GuKang. The control group was given the intragastric administration with estradiol valerate, After treatment for 1 week, blood sampling was used to separate plasma.1 day old SD rats were used to culture osteoblasts, which were divided into3 groups:normal group, control group and observation group, normal group was not added any drug-containing plasma, observation group was added culture medium of drug-containing plasma of GuKang, control group was added culture medium of drug-containing plasma of estradiol valerate. after 1 week, MPTP,SDH of osteoblasts were measured.Result:1. Femoral BMD:normal group was higher than ovariectomized group (P<0.01); There was a statistically significant difference that the value of observation group, of the control group respectively compared with the ovariectomized group (p<0.05).2. In MPPT,Cyt C and SDH of skeletal muscle, OD ratio of normal group, control group and observation group were lower than ovariectomized group in MPTP(P<0.05). But there was not a statistically significant difference that the content of observation group, of the control group and of normal group respectively compared with the ovariectomized group in Cyt C (P>0.05). There was a statistically significant difference between observation group and ovariectomized group (P<0.05). There was not a statistically significant difference normal group and ovariectomized group(P>0.05).3. In-SOD and MDA, there was a statistically significant difference between normal group and ovariectomized group (P<0.05). There was a statistically significant difference that the value of observation group, of the control group respectively compared with the ovariectomized group (P<0.05).4. In MPTP and SDH of osteoblast, there was a statistically significant difference that the RFU of observation group, of the normal group respectively compared with the control group (P<0.05). But there was not a statistically significant difference that the content of observation group, of normal group respectively compared with the control group in SDH(P>0.05). Conclusion:GuKang could improve BMD of Ovariectomized Rats. Through intervention of GuKang, the permeability of MPTP in skeletal muscle and osteoblast can be controlled. In addition it could improve the content of SDH and reduce the content of MDA, which.can clean up free radicals to inhibit cell apoptosis. These researches could provide the objective reasonable quantification basis for mitochondrion as a therapeutic target from the theory of "kidney controlling bones, spleen controlling muscle and spleen-renal interrelationship" in the treatment of osteoporosis.