| Background:Previous studies found that some bone morphogenetic proteins(BMPs) can induce mesenchymal stem cells to differentiate into osteoblast, but there is no report about the ability of BMP9 to induce skeletal muscle-derived stem cells ( MDSCs) to differentiate into osteoblast.Objective: To assess the capacity of BMP9 induced skeletal muscle-derived stem cells into osteoblasts.Method: Rabbit skeletal muscle-derived stem cells were obtained by the two—step method of collagenase-I and trypsin, and transfected by the BMP9 through recombinant adenoviruses. Then observe the effect of BMP9 induced skeletal muscle-derived stem cells into osteoblasts by testing the alkaline phosphatase quantitation and calcium deposition.Results and Conclusions: Muscle-derived stem cells were successfully isolated from the rabbit and over 80% of Muscle-derived stem cells showed Sca-1 positive immuno reactivity. BMP9 induced alkaline phosphatase activity in skeletal muscle-derived stem cells and promoted calcium deposition of in the cells.Compared with the control group, the quantity of alkaline phosphatase and calcium deposition for BMP9 group are increased obviously(P<0.01) . From the above, BMP9 has the capability to induce the skeletal muscle-derived stem cells into osteoblasts. |
