| With the the ocean exploitation, the abundance of marine drug resources provided the Traditional Marine Chinese Medicine (TMCM) with a broad prospect. It became an essential issue to promote the development of marine drug resources and apply them on clinic for the oceanic scientists. Many compounds were obtained from the marine organisms after the separations of the marine drug resources. A lot of compounds were tested for various activities but a few of them became the candidates and were studied on clinic which may be still condemned at last, therefore, lots of marine drug resources couldn't be used on clinic.The theory of Traditional Chinese Medicine is an unique theory in China and played an important role on the health of the people in China for thousands of years. The modern analysis and application of the theory of Traditional Chinese Medicine may play a huge role in promoting the development of Marine Chinese Medicine. Meridian Tropism Theory (MTT) is an important part of the theory of Traditional Chinese Medicine which can not only instruct rational use of drugs in clinic practice but also develop the potential efficacy of the drug. So applying the MTT to develop the marine drug could not only improve the target of the marine drug but also expand the screening range of the marine drug.The study was based on the preliminary work of our laboratory and selected Baibu (Stemona tuberosa Lour.) which was a traditional drug of Lung Meridian Tropism to study its chemical components such as mineral elements, amino acids, polysaccharides. alkaloids and especially its migration component in plasma. The results showed that the stemoninine was its migration component in plasma. The pharmacokinetics of stemoninine showed that its in vivo behavior could be described with two-compartment mode and the Tmax and Cmax were 1.67±0.11 h and 44.35±4.67μg/mL after oral administration of STE. The bioavailability of stemoninine was 12.41%. The tissue distributions showed that the stemoninine could distribute in the tissues and achieved the max concentration in lung at 90 min which suggested that the distribution of stemoninine was targeted. The result was concordant with the traditional judgement of Baibu. Besides, the stemoninine could permeate the blood-brain barrier and achieve the max concentration in brain at 120 min. The stemoninine was reported to exert its antitussive action via both central and peripheral actions which concordant with its tissue distributions.Base on the studies of the Baibu, a continue study was carried out on the complex prescription "Zhi Sou San" which contents the Baibu. The influence of the complex prescription on the meridian tropism on Baibu was approached. The pharmacokinetics results showed that the in vivo behavior of stemoninine was still two-compartment mode and the Tmax and Cmax were 1.30±0.27 h and 47.57±9.61μg/mL after oral administration of "Zhi Sou San". The bioavailability of stemoninine was 19.19%. The tissue distributions showed that the stemoninine could distribute in the tissues and also achieved the max concentration in lung at 90 min and in brain at 120 min. The results showed that the complex prescription could promote the bioavailability and tissue distribution concentrations but not change the meridian tropism on Baibu.The pharmacodynamic components of the "Zhi Sou San" were summaried and the results showed that alkaloids, flavones, steroids and terpenes were the pharmacodynamic foundations of the "Zhi Sou San". After a summary of relationship between the chemical structures and activities, two marine Chinese medicine Chengliu (Tamarix chinensis Lour.) and Haizao (Sargassum pallidum (Turn.) C. Ag.) were selected to replace the Chenpi (Citrus reticulata Blanco.) and Baiqian (Cynanchum stauntonii (Decne.) Schltr. ex levl.) and a new complex prescription was made which was called "Hai Yang Zi Ni Fang". The continue study was similar to the study of "Zhi Sou San". The influence of the new complex prescription on the meridian tropism on Baibu was approached. The pharmacokinetics results showed that the in vivo behavior of stemoninine was still two-compartment mode and the Tmax and Cmax were 1.20±0.27 h and 45.16±9.74μg/mL after oral administration of "Hai Yang Zi Ni Fang". The bioavailability of stemoninine was 19.29%. The tissue distributions showed that the stemoninine could distribute in the tissues and also achieved the max concentration in lung at 90 min and in brain at 120 min. The results showed that the new complex prescription could also promote the bioavailability and tissue distribution concentrations but not change the meridian tropism on Baibu.Three kinds of pharmacological activity including antitussive, antiasthmatic and expectorant were selected to compare the "Zhi Sou San" with "Hai Yang Zi Ni Fang" The results showed that the two complex prescriptions were similar on the pharmacodynamic actions except for the antiasthmatic, of which the latter was more powerful than the former. The result suggested that the combination of Chengliu and Haizao had an important contribution to the antiasthmatic activity of the "Hai Yang Zi Ni Fang".The above results showed that it was feasible to the judge the meridian tropism on Baibu by the tissue distributions of the pharmacodynamic component. The complex prescriptions could promote the targeted distribution of stemoninine which suggested that the complex prescriptions could enhance the meridian tropism action of Baibu. After replacing marine Chinese medicines which had similar chemical structures and pharmaco-activities with the land medicines, the complex prescription could display a similar even more powerful pharmacodynamic action, which provided a brand-new path to utilize the marine drug resources with much efficiency.
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