Studies On Kidney Failing To Store Essence In Behavioral Model Of Two Generations And Its Gene Expression Profile

BackgroundThe ring will move over a few years time, December 26,2004 Indian Ocean tsunami killed 22.5 million people, the natural disasters has left a heavy human thinking; shocked the world, "9.11" incident, it is man-made the shadow of disaster to humanity; 12 May 2008 Wenchuan earthquake has once again sounded the alarm:disaster-will be accompanied by the pace of human progress! Natural disasters, human beings can be formed for different degrees of damage, not only by direct stimulation of the parties, their offspring will also have an indirect negative impactParticularly in the period of individual life and health related pregnant women, fetuses, newborns, infants and young children most affected, the consequences of bad. Assess and mitigate earthquake disasters in pregnant women, production and new-born, infants and young children has become an urgent and serious Jun reality. Although many people made the observation, also tried to simulate earthquakes were a number of simulated earthquake test, go into the mechanism of the earthquake. However, the existing analog seismic experiments, mostly geophysical experiment, difficult to simulate earthquake for biological experiments, more difficult to transfer seismic field Investigation, to study the combination of both. This paper paid a heavy price of 5·12 Wenchuan earthquake in human resources, the simulated experimental study of earthquake seismic nurtured on mother and daughter and physiological behavior of the danger and severity of adverse effects, fear of earthquakes as a stress posttraumatic stress disorder (PTSD), on human and animal physiology and behavior of internal principles.Objectives ①Used to pay a heavy price for the 5·12 earthquake resources, earthquake revealed women of childbearing age, pregnant women and newborns have a negative impact of the characteristics of law;②Biological experiments by simulating the earthquake, seismic exploration capability and mice bred offspring biological characteristics, growth, development and behavior patterns;③Screening of the offspring based on gene expression differences in genes critical to explore the "virtual mother and child" of molecular biological mechanisms.Methods①"Kidney essence is not hidden" literatureancient, modern and contemporary to "kidney essence Zhi", "main reproductive kidney, the main growth", "as the birth of the kidney" and other kidney has a very rich theory of and research, and summary of "kidneys" in the seismic stress state involved m stress responses.②Macroscopic behavior of mother and two generations of Statistics and Analysis of PhysiologicalAccording to "health care facilities in Sichuan hospital delivery register," 5·12 earthquake to collect heavy, heavy, light, etc.7 before the earthquake disaster area after the earthquake record of delivery, application spss13.0 multi-channel analysis of statistical software; the same time to simulate seismic study of biological experiments, the mice two generations of mother and child related information, mining seismic events as a catastrophic stress on the mother and two generations of behavior studied.③Microscopic features of gene microarrayUsing gene chip technology, the offspring during pregnancy and pregnancy earthquake group 2 group of non-seismic design of gene expression profiling test components, using SAS, FatiGO + and many other databases screening effect of earthquakes on the characteristics of offspring gene expression.ResultsO field research of 69822 cases in the four degree disaster areasEarthquake on the fetus of the degree of stimulus intensity relationships, often expressed in males, premature child ratios and birth defects in the proportion of the earthquake group and after the earthquake group than before the earthquake group there were significant differences, some of neonatal length, weight also change.②six groups in two approveds of the seismic experiment Simulated earthquake by carrying out biological experiments and found that chronic stress adversely affect reproductive function in mice. Ability of specific performance marks the decline in primary mouse embryo and growth retardation, some behavioral retardation, low immunity, even for the normal development of the brain affected offspring.③Gene expression profiles of seismic stressBody response to stressful events, altered expression of many genes (including up and down), accompanied by a number of channel changes, especially disease-related pathway to a maximum of (birth defects and congenital diseases); followed for the signal transduction system and the endocrine system-related immune pathway.DiscussionO Simulated earthquake biological experiment is feasible, broaden the simulated earthquake test applications;②Interpretation using traditional Chinese medicine theory, "earthquake" for pregnant women and women of childbearing age two generations of mother-child mechanism;③Reaction and number of progeny differences in gene expression changes related to multiple channels.