Radiata Is A Plant Flowering Control Technology And Flowering Physiology

Lycoris Amaryllidaceae is bulb flower and a kind of excellent ornamental plant which has been applied in garden landscape and cut flower. It sprout in early spring or shoot out leaves after flowering in fall, and keeps on green until early summer, and it effloresce in summer when flower is lack. The flower stem shoot out from nake ground and flower are beautiful and courlorful, so it is significant potential to study its flowering time regulator. And meanwhile modern flower industry bring forward high demand for the flowering time of ornamental which directly contact with the market time, business value and variety cultivate and so on. Therefore flowering time regulator has become an important nucleus technic in ornamental managing.Using L.longituba, L.sprengeri, L.radiata as material in Nanjing, aim at climate characteristic and ecology and biology speciality of Lycoris in Nanjing, researched from several aspect:(1) Study on flower bud differentiation of L.radiata. Observed flower bud differentiation through paraffin method at the same time, measurate endogenesis hormone content and nucleic acid content in different stage in order to see the change of physiology during flower bud differentiation. The result shows nucleic acid content in bulb bud is ascending during flower bud differentiation course, and reduce IAA and ABA content accelerate inflorescence shape, high ABA and IAA content accelerate flower bud gestate. ZR content is always in low level before flower bud differentiation, and then rised in anaphase; GA content shows no directly correlation with flowering. Maybe the four hormone correspond to accelerate flower development.(2) Florescence mode. Observing florescence rule during flowering time. Result shows that the three species demand different day average temperature. L.longituba demand the highest temperature in flowering time, so it flower in the highest temperature during one year. The next is L.sprengeri and L.radiata. And the three species show different standing time during flowering fastigium. The longest is L.longituba which can standing six days. In addition the wide and high of stem diametr of the three species is different, L.longituba is far exceed L.sprengeri and L.radiata. The neck stem diameter of the three species stop growing after shoot out ground, and the ground stem diameter stop growing after the first fleuret open.(3) The change of physiology and ultrastructure of cell during senescence of L.longituba. This study mensurate the index of physiological and observed ultrastructure of cell in four different period in senescing corolla of Lycoris longituba. The result shows that from florescence to senescence, soluble protein content in petal declined gradually, but MDA content and membrane relatively permeability increased gradually. However there is no negative correlation between soluble protein content and concentration free amino acid is possibly related to nutrient exhausting. Meanwhile, with the senescence of petal, the cytoarchitecture disassembled, nucleus distortion, cell wall separate from cytoplasm, but endoplasmic reticulum show no destroyed obviously. Therefore, senescence for Lycoris longitube. is caused byprogrammed cell death controlled by many factors.(4) Treatment with plant growth regulator. After soak L.radiata bulb with GA, Ethrel, boric acid, mensurate physiology index in the leaves. Result shows that applicate of Ethrel within the concentration of 100-200mg/L and 50~200mg/L GA3 increase chlorophyll content. All concentration of Ethrel reduce carotenoid content. Boric acid of concentration of 150mg/L and 200mg/L increase carotenoid content. Ethrel increase MDA content and reduce SOD activity. GA3 is in opposition with Ethrel. With the treatment concentration of Ethrel increase, GA3 and IAA content in leaves reduced gradually. ABA content in leaves is the highest when Ethrel treat with 150mg/L. The higher concentration of GA3, the higher GA3 content and the lower ABA content in leaves. IAA content in leaves are significantly increase by applicati...