Effect Of Complex Prescription Herba Siegesbeckiae Capsule On The Express Of Cytokine And COX-2 Of Acute Gouty Arthritis In Rats

Objective The aim of the study is to research the mechanism of therapyaction for acute gouty arthritis through complex prescription herbasiegesbeckiae capsule.Methods The herb is used in the model of animal which get acute goutyarthritis. The method of the tumefaction on the toe of rats test, pathologicalexamination, ELISA euzymelinked immunosorbent assay, Immunohistochemistry, RT-PCR are used to determine the cytokine level andCOX-2, VCAM-1, NF-KB expression.We compare the treatment effect in rats ofherb to the effect of anti-inflammatory(Diclofenac) and evaluate the herb'scurative effect.Results1. The complex prescription herba siegesbeckiae capsule have evidentanti-inflammatory effect and can significant restrain the arthrocele ofankle-joints of rats which get acute gouty arthritis. The restrain effect is inferiorto the effect of Diclofenac, but herb is more safe and has no adverse effect..2. The complex prescription herba siegesbeckiae capsule can significantrestrain leukocytic infiltrate of the rat's affection joints and the accrementitionof synovial cell of the rat's affection joints.3. The complex prescription herba siegesbeckiae capsule can significantcut down the contents of beta-imidazolyle thylamine, IL-1β,IL-8,TNF-αofthe rats's affection joints membrana synovialis architecture, which can also cutdown COX-2, VCAM-1, NF-κB expression, then restrain the inflammation ofmembrana synovialis architecture.Conclusion The complex prescription herba singesbeckiae capsule'santi-inflammation and analgesia mechanism may be related to inhibiting thebeta-imidazolyle thylamine, IL-1β,IL-8,TNF-αexcreting, acceleratingCOX-2,VCAM-1 and NF-κB expression.