The Influence Of Intraocular Surgery On ACAID In Mice Model

锘库槄Anterior chamber associated immune deviation (ACAID) plays a key role in the ocular immune privilege. It is one of the important reasons of the high success rate in corneal allografts. The existence of ACAID depends on intact blood-ocular barriers, an absence of blood and lymph vessels in the graft and its bed and soluble immunomodulatory factors in aqueous humor. Because intraocular surgeries must break the blood-ocular barriers and may also change the way of aqueous humor flow. So It was possible that intraocular surgeries might destroy the ACAID. Moreover, the numbers of corneal allografts after the intraocular surgeries and combined intraocular surgeries with corneal allografts are increasing recently. So It is necessary to investigate the influence of intraocular surgeries on the ACAID. The results will be helpful to choose the time of corneal transplantation and to direct the medicine treatment after the transplantation. In this study we made two kinds of intraocular surgeries models which were trabeculectomy, lens and vitreous resection to evaluate the influence of intraocular surgeries on the ACAID. The results indicated that the post-surgery eyes lost the ability to induce ACAID in the research duration of this study, but the results also indicated the trend that the ability to induce ACAID might recover with the time. According to the primary results, we thought the combined intraocular surgeries with corneal allografts and the corneal allografts after the early stage of the other intraocular surgeries should be avoided.鈽匬art鈪?鈽匱he Influence of trabeculectomy on ACAID in Mice Model鈽卲urpose: To study the changes of the ability to induce the ACAID in Mice trabeculectomy model and the relationship with the post-surgery duration.鈽匨ethods: Wistar mice were chosen to involve this study. After the surgeries, the mice were randomly divided into 5 groups. Other 5 SD mice and 1 wistar mouse were used as the providers of epithelium removed corneal grafts to induce ACAID. Group A: corneal grafts from wistar mouse were implanted into the anterior chamber 1 week after the trabeculectomy. Group B: the spleen cells were injected into the nape of neck 1 week after the trabeculectomy. The corneal grafts from SD mice were implanted into the anterior chamber 1 week, 4 week and 8 week after the trabeculectomy in group C, D, and E respectively. To induce the DTH, spleen cells were injected into the right ear pinna 1 week after the neck injection in group B, 2 weeks after the corneal grafts anterior chamber implantation in other groups. At the same time point as the DTH inducement, heart blood was collected to detect the concentration of IL-4 and IL-10．And the spleens were removed to evaluate the expression of the GATA-3 mRNA by RT-PCR. The eyeballs were enucleated to evaluate the histopathologic changes.Results: 1) The evaluation of DTH. The DTH were found in group B, C, D, and E. 2) The analysis of the IL-4 and IL-10 concentrations. The serum concentrations of IL-4 and IL-10 were statistically different in group C, D, and E (p<0．001) . The concentrations of IL-4 and IL-10 were significantly lower in group D than those in group C and E. The trend that the concentrations of IL-4 and IL-10 increased in the early stage after the surgeries, then decreased, and finally increased again was observed. 3) The expression of GATA-3 mRNA in spleen. An obvious up-regulation of the GATA-3 mRNA was noted in group D and E. 4) Histopathologic changes of the eyeballs. The exudation and few inflammatory cells in the anterior chamber were observed in group C. No obvious exudation and inflammation in anterior chamber were found in group D and E.Conclusions: The eye after the trabeculectomy lost the ability to induce ACAID in the research duration of this study. But with the gradually decrease of the intraocular inflammation and the increase of the levels of IL-4, IL-10 and GATA-3 mRNA, the ability to induce ACAID might recover with the time.PartⅡThe Influence of lens and vitreous resection on ACAID in MiceModelpurpose: To study the changes of the ability to induce the ACAID in Mice lens andvitreous resection model and the relationship with the post-surgery duration.Methods: Wistar mice were chosen to involve this study. After the surgeries, themice were randomly divided into 5 groups. Other 5 SD mice and 1 wistar mouse wereused as the providers of epithelium removed corneal grafts to induce ACAID. GroupF: corneal grafts from wistar mouse were implanted into the anterior chamber 1 weekafter the lens and vitreous resection. Group G: the spleen cells were injected into thenape of neck 1 week after the lens and vitreous resection. The corneal grafts from SD mice were implanted into the anterior chamber 1 week, 4 week and 8 week after the lens and vitreous resection in group H, I, and J respectively. To induce the DTH, spleen cells were injected into the right ear pinna 1 week after the neck injection in group G, 2 weeks after the corneal grafts anterior chamber implantation in other groups. At the same time point as the DTH inducement, aqueous humor was collected to measure the concentration of TGF-β2 and IL-10; heart blood was collected to detect the concentration of IL-4 and IL-10．And the spleens were removed to evaluate the expression of the GATA-3 mRNA by RT-PCR. The eyeballs were enucleated to evaluate the histopathologic changes.Results: 1) The evaluation of DTH. The DTH were found in group G,H,I and J. 2)The analysis of the TGF-β2 and IL-10 concentrations in aqueous humor and the IL-4, IL-10 concentrations in serum. Significant differences were found in concentrations of TGF-β2 and IL-10 in aqueous humor and the concentrations of IL-4 and IL-10 in serum in group H, I, and J (p<0．001) . The concentrations of TGF-β2, IL-4 and IL-10 were significantly lower in group I than those in group H and J. The trend that the concentrations of TGF-β2, IL-4 and IL-10 increased in the early stage after the surgeries, then decreased, and finally increased again was observed. 3) The expression of GATA-3 mRNA in spleen. An obvious up-regulation of the GATA-3 mRNA was noted in group I and J. 4) Histopathologic changes of the eyeballs. The exudation and few inflammatory cells in the anterior chamber were observed in group H and I. No obvious exudation and inflammation in anterior chamber were found in group J. Conclusions: The eye after the lens and vitreous resection lost the ability to induce ACAID in the research duration of this study. But with the gradually decrease of the intraocular inflammation and the increase of the levels of TGF-β2, IL-4, IL-10 and GATA-3 mRNA, the ability to induce ACAID might recover with the time.