Transplantation Of Autologous Peripheral Blood Stem Cells For The Treatment Of Severe Limb Ischemia

Objective Treatment of severe limb ischemia (SLI) remains a clinica) challenge. Preclinical studies and one pilot study have shown that local bone marrow cell implantation induces a neovascular response with a significantly increased blood flow into the ischemic limbs. To develop a more effective approach, we at first evaluated the clinical efficacy of autologous transplantation of mobilized peripheral blood stem cells (PBSCs) for the treatment of CLI. Methods In the two cases of SLI as control group, rhG-CSF 600 μg/day for 5 days to mobilize stem cells. In the patients with SLI, including 7 cases of severe lower limb arteriosclerosis obliterans, 10 case of severe lower limb ischaemia in diabetes and 4 cases of severe thromboangiitis obliterans as transplant group, PB-SCs were collected with a Version4 blood-cells separator after 5-days rhG-CSF mobilization. Three hours late, the PB-SCs were intramuscularly injected into the ischemic areass of the limbs (3×10~9cells per limb). About forty days after the implantation, the severe diseased limb was given an additional implantation of the same number of cells as the first. The clinical and laboratory findings...