| The hepatic fibrosis (HF) refers to the textile fiber of the liver which resulted by each kind of pathogenic factor knot to contract the organization unusual proliferation. The main pathology characteristic is extracellular matrix (ECM) that synthesizes by the collagen Extracellular matrix increases, degrades the relative insufficiency, excessively many depositions in liver, thus forms HF. The chronic disease of the liver caused by each kind of cause of disease can appear the liver fibrosis. if further development, it causes the hepatic lobe reconstruction, the vacation small leaf and the tubercle formation, then enters liver Because the Chinese traditional medicine has the multi-components, the multi-targets, the multi-way function characteristic, unfolds the superiority in the anti-HF aspect, has demonstrated the good prospect.HF belongs to Chinese medicine" liver zhao", the "liver accumulating" , "jaundice" , "costal pleurisy" , "agglomerates illness and so on categories. The Chinese medicine believed that, the cause of the disease pathogenesis is the feeling hot and damp epidemic disease poison, the diet is inappropriate, causes the liver to release the function to be abnormal sparsely, the vital energy of the liver stagnates strongly fragrant, blood line of impeded;The vital energy of the liver violates the spleen earth horizontally, spleen does not transport, the phlegm wet in lives . Forms the liver spleen same sickness, the liver strongly fragrant spleen weakness pathological change. The hot and damp epidemic disease poisonous pent up, further hinders the vitality body fluids movement, produces the phlegm wet, pathology products and so on;Gets sick lingers evilly, the damage healthytendency, causes the liver the cloudy blood to be insufficient, the spleen kidney is weak, thus forms, the heat, the poison, the stasis, the phlegm wet ties mutually, the liver, the spleen, the kidney are insufficient pathological change. This empty sign reality, actual situation mixture are the pathogenesis characteristics which this gets sick, the two cause and effect relationship, penetration pathological change throughout.Zhuangganzhuyujian by the stasis decease country' s name old doctor of traditional Chinese medicine. Chinese medicine disease of the liver expert Professor Lin Peixiang treats the chronic hepatitis, hepatitis liver cirrhosis passes through the proven prescription effectively, by the ganoderma lucidum, radix astragali, the Chinese angelica, twists the stock blue, fries the turtle armor, the ground beetle insect, radix notoginseng, rhizome polygonicuspidati and so on giant knotweed Chinese native medicine group Fang Ercheng. The earlier period clinical research indicated that, the strong liver fries by the stasis to HF, the chronic hepatitis some biochemistry target has improves the effect well, can obviously improve the patient clinical symptom, has the anti-virus, guarantees the liver, the promotion collagen textile fiber degrades, functions and so on the suppression liver inflammation activity, also not obvious poisonous side effect. The experimental study indicated that, it can obviously reduce HF rat blood serum HA, LN, IV- C, PCIIIThe level, suppresses HF rat HSC the activation and the multiplication, reduces the liver inflammation and the fibrosis degree, has the anti-HF function.The old doctor of traditional Chinese medicine experience is the motherland medicine precious inheritance, take the Chinese medicine theory as the instruction, the utilization modern biological science technology, conducts the research to an old doctor of traditional Chinese medicine after the proven prescription, is advantageous treats HF in the discussion Chinese native medicine the function mechanism and an old doctor of traditional Chinese medicine treats the disease of the liver the academic theory;And can accumulates for anti-HF and other organization fibrosis Chinese medicine research may supply the model the research mentality, for uses the modern scientific theory to expound the Chinese medicine action mechanism research provides a good breakthrough point. At present, take the cell factor as the breach, using the molecular biology method expounded the HF formation mechanism, is the anti-HF research direction. TGF-P, is most importantpresses HF to form the factor, its biology effect oughts with the cell membrane in specificity acceptor (TPR) unifies can display. This research discussed the strong liver to fry the influence by the stasis which TGF-3 1 and the acceptor expressed to the HF rat, was for the purpose of to we anti-HF drug efficacy and the possible function machine manufacture further research. The laboratory procedure and the method are as follows: Selects health clean level Wistar rat 180, half female and male, body weight 200~220g, takes 24 to take the normal control group stochastically (A group). The surplus rat carries on HF to make the mold. Uses the CC14 compound factor to make the mold . Gives rat food with high fat and low protein (take corn flour as feed, adds 0.5% cholesterol, tests 1, 2 weeks adds 20% lard), 10% ethyl alcohol are the only drink, hypodermic injection CC14 (1st time uses 5ml/ kg body weight, later 3 day hypodermic injection 40% oil emulsion CCl43ml/kg body weight). In order to prevent the liver nature repair to tests the result the influence, each group started still each week after the medication to inject 40% CCL4oil emulsion 3ml/kg body weight 1 time (A group of exceptions). In the end of 4W, makes the mold rat also to remain 107, after A group and makes the mold rat each to execute 8 to confirm stochastically HF forms makes the correlation detection. From makes in the mold rat to take 96 stochastically, divides into the pathology model group stochastically (B group), the strong liver fries the granular formulation large dose by the stasis (adult 20 time of quantities), the dosage (adult 10 time of quantities), the small dosage (adult 5 time of quantities) treats the group (C, D, E group), the colchicine control group (F group), dahuangzhechongwan (DHZCW) control group (G group) the altogether 6 groups, each group of 16 rats. After makes mold 4W each group to give to p. o separately to the medicine, A group of normal feeds feed, p. o physiological saline that the cubqge is same as treatment group co-content;B group administrates physiological saline that the cubqge is same as treatment group co-content;C, D, E group give the strong liver to fry by the stasis exempt fry the granular formulation, the dosage respectively are lOg/kg body weight, 5g/kg body weight, 2. 5g/kg body weight;F group fills the clothing colchicine 100ug/kg body weight;G group fills clothing DHZ.CW" 1. 5g/kg body weight. Above each group fills the gastric juice amount of $pace occupied is lOml/kg body weight, gives the medicine 1 time per day, its density and the equivalent dosage according to the body surface area conjpersioj*,altogether give medicine 12W.A, B, C, D, E, F, G each group after the medication treatment 6W, 12W, fasts separately 12h, selects 8 big mice to call stochastically heavily, the femoral artery picks the blood, after the blood specimen room temperature laying aside approximately 1 hour 1500rpm/min centrifugal 15~20min, the absorption upper formation blood serum carries on the examination. After picks the blood to execute the animal, splits takes the liver, the spleen, and takes the liver right leaf same spot to organize 2, the accurate name takes approximately 200mg on the electronic balance, then puts in it in the ep tube, the serial number, and joins 400ul the trizol solution, after the seal membrane seal, puts in the liquid nitrogen to save frozen, prepares withdraws RNA. Another fixes in 10% formaldehyde, paraffin wax embedding, slice, distinction line of HE, VG, immune histochemical dyeing.The observation target is as follows:1. Rat ordinary circumstances observation;The rat liver, the spleen organization shape observation, measured roughly the liver, the spleen weight, calculates the liver spleen index (the internal organs index = internal organs weight/Body weight).2.Light microscope observation liver organization liver cell denatured degree, HF degree.3.Account for the total field of vision area ratio with the MIAS image analysis system computation liver organization collagen area and the collagen area.4. Determines the liver with the MIAS image analysis system under 40X10 the enlargement factor to organize Des and a-SMA the masculine cell number and the masculine region area ratio.5. The double immune body contains filling the ABC-ELISA law examination blood serum TGF-3 1 expression difference.6. RT-PCR technical examination liver organization TGF- 0 1 mRNA expression difference.7. Real-time fluorescence quota PCR technology examination liver organization TPR-I, TPR-II expression difference.The findings are as follows:1. The rat ordinary circumstances observation demonstrated that, The strong liver fries by the stasis may obviously improve the HF rat's state of mind,the diet, the superficial knowledge luster, increases its body weight.2. The rat liver, the spleen organization roughly shape observation and the light microscope observation demonstrated that, The strong liver fries by the stasis can obviously reduce the rat liver organization inflammation activity and the fibrosis degree, manifests strongly guarantees the liver, the anti-HF function.3.The liver, the spleen weight determination and the liver spleen index computed result showed that, The strong liver fries by the stasis can reduce the liver, the spleen weight, reduces the liver, the spleen index.4. Account for the total field of vision area ratio with the MIAS image analysis system computation liver organization collagen area and the collagen area the result to demonstrate that, The strong liver fries by the stasis can reduce the liver organization collagen total area and the collagen area occupies the total field of vision area the proportion.5. Organizes Des with the MIAS image analysis system determination liver and a -SMA the masculine cell number and the masculine region area ratio result demonstrated that, The strong liver fries by the stasis can reduce Des and a-SMA the masculine cell number and the masculine region area proportion.6. TGF-^iand the liver organization TGF-PimRNA expression findings showed to the HF rat blood serum that, The strong liver fries by the stasis can reduce in the blood serum TGF-Pi level, suppresses liver organization TGF-p,mRNA the expression.7. To HF rat T P R- I T ï¿¡ R-IIThe expression research demonstrated that, The strong liver fries by the stasis can suppress T0R- I TPR-IIThe expression, big, the middle monitoring team' s improvement effect is best, with colchicine drug efficacy quite, three non-significance difference;Small monitoring team and DHZCW drug efficacy quite, the two non-significance difference.8. The strong liver the drug efficacy which fries by the stasis has certain dosage dependence relations: Big, the middle monitoring team curative effect surpasses the small monitoring team, the big monitoring team surpasses the monitoring team slightly. Relates not obviously with the treatment course.Research conclusion:1. Zhuangganzhuyujian by the stasis can obviously reduce the rat liver organization inflammation activity and the fibrosis degree, presents the good local constable liver, the anti-HF function.2. Die starken Leberfischrogen durch das stasis konnen das ganze Gebiet des Leberorganisation Kollagens verringern und der Kollagenbereich besetzt die Gesamtmenge auffangen vom Anblickbereich den Anteil, Aufforderungen hat die Ausgleichkollagen-starke Verbreitung Funktion.3. The strong liver fries by the stasis can reduce Des and a-SMA the masculine cell number and the masculine region area proportion, thus suppresses HSC the activation, the multiplication.4. Zhuangganzhuyujian by the stasis can reduce in the blood serum TGF-p i level, suppresses liver organization TGF- P lmRNA, T 3 R- I T3 R-II Expression, thus suppresses TGF-3 1 presses HF to affect.5. Suppresses HSC the activation, the multiplication, reduces in the blood serum TGF-Pi level, suppresses liver organization TGF-P,mRNA, T 3 R- I T 3 R-IIThe expression, possibly is the strong liver fries anti-HF by the stasis the main mechanism.6. The strong liver the function which fries by the stasis for raises the liver the cloudy blood, is good for the spleen invigorates the kidney, blood circulation removing extravasted blood dispels phlegm, refrigeration disintoxicating. The clear heating wet disintoxicating may shut off the cause of disease, blood circulation removing extravasted blood dispels phlegm insolubly already the collagen which forms, raises the liver to be good for the spleen invigorates the kidney may enhance organism resistivity, protects the liver cell shape and the vigor, promotes the liver cell regeneration;Suppresses HSC the activation, the multiplication;Suppression TGF-3imRNA, T 3 R- I T 3 R-II Excessive expression.
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