Study On The Glycosaminoglycans From Ray

There are three parts in this dissertation. Studies on isolation and structure characterization of Ray cartilage glycosaminoglycans(RCG) were discussed in the first part. Studies on the preparation of Ray Cartilage polysaccharides and its injection were discussed in the second part. The third part mainly concerned on studies on pHarmacodynamics of Ray cartilage glycosaminoglycans.1 Studies on isolated of RCG and its structure characterizationFor the first time, Two homogenous glycosaminoglycans (RCG I and RCGII) was isolated from Dasyatidae. akajei ( MulleretHenle ) after extraction by alkali , deposition on grade by acetone , deproteinization, Sephadex G-75 elution. Through analisis of 13C-NMR, GC, GC-MS, IR and HPLC , Its means molecular weight was estimated to be 9. 7 104and 1. 8X 105 respectively from the standard curve with Detran T system determined by HPLC method. Suger analysis showed RCG I and RCGII were composed of -D-galactosamine , -D-glucuronic , -D-galactose and 3 -D-glucose in 1, 4 linkage. The molar ratio of galactosamine and glucuronic is 1:1. Methylatin analysis showed the sulfate was at 0-6 of galactosamine , galactose and glucose. The configuration is confirmed by IR and NMR data. The analysis of NMR showed that there was acetyl at amidocyanogen of galactosamine. Galactose and glucose of RCG I and RCGII was respectively in the molar ratio of 1:2.5, 1: 5. These results showed that the structure of RCG I is consistent with RCGII, there was tiny difference at terminal linkage chain with protein.The proliferation of EC was controlled by RCG, this showed that RCG was one of active component of Ray cartilage.2 Studies on preparation and injection of RCG and its quality standardAccording to the orthogonal test, preparative technological process of RCG was defined. The optimum preparative technological process was that extractionwith 4% NaOH, two times and 18 hours, the protein of extractive hydrolyzed by acid , trypsin and salt, deproteinization by argil and active carbon, deposition with enthanol, dehydration with enthanol and acetone , dryness. The injection of RCG was made of the material. The material was solve with water, putting into NaCl to adjust penetration press, through filtration, dispart, extinguish microbe process, becoming into production.To established the general check standard and method of content determination. To measure content of glucuronic , in order to control the content of RCG in preparation. The method of content was checked from precision , repetition , stability and recovery .the results showed that the method of content accord with request.3 Studies on anti-tumor and cholesterol-lowering research The results of pHarmacodynamics experiments showed that RCG could anti-tumor clearly , and promote immunological function, decrease blood-lipid obviously. RCG could control collagenase and control the proliferation of EC.