The Experimental Research On Alterations Of Ca～(2+) Steady-state Regulation And Effects Of SODmRNA Expression In Rat Brain Cells After Intracerebral Hemorrhage (ICH) And Intervention Of Acupuncture

Obective: To study the mechanisms of acupuncture following intracerebral hemorrhage(ICH) and alterations of brain cell calcium inos regulation, actions of intracellular Ca2+ concentration in isolated ratbrain cell effects of SODmRNA expression in rat brain cell and its relationship with cell calcium and SOD, It provides scientific basis for clinical from the areas of cytobiology and molecular biology. Methods: Rat model of cerebral hemorrhage was made with collagenase, The rats were divided into 4 groups: the normal group, the sham experiments group, the intracerebral hemorrhage(ICH) group, the acupuncture group. According to the time of the intracerebral hemorrhage(ICH) formation, the intracerebral hemorrhage(ICH) group and acupuncture group were divided into 5 groups respectively, the groups of 5^ 15>3(K6(K 180 min are studied the influence of cell calcium ions and intervention of acupuncture ,the groups of 3 hour % 6 hour > 24 hour> 3 day > 7 day are studied the effects of SODmRNA and intervention of acupuncture.Brain cell were isolated from rat brain with Fura2-AM measurements of intracellular Ca2+ concentration([Ca2+]j). The distribution of Ca2+ and changes of ultrastural in brain neuron were determined by using K-pyroantimonate cytochemistry method, in the mean time, the energy-dispersive X-ray microanalysis method was used to measure the intracellular Ca2+ concentration. The SODmRNA expression in brain tissue were observed with In situ hybridization method.Resuts: The Ca2+ concentration and distribution of the endoplasmic reticulum in brain cell was obvious at 5 min after intracerebral hemorrhage(ICH), descended rpidly at 30 min, peaked at 60min, then risen gradually. The Ca2+ concentration and distribution of the cytoblast, cytoplasm and mitochondria in brain cell was obvious at 5min after intracerebral hemorrhage(ICH), rose rpidly at 30 min, peaked at 60 min, then descended gradually. The changes of ultrastructral in neuron was obvious at 5 min after intracerebral hemorrhage(ICH), peaked at 60min and alleviated gradually. Calcium ions fluxes from the extracellular fuid into the intracellular space may trigger irreversible injury that is the endoplasmic reticulum expind, mitochondria swelling at 5 min, karyopyknsis, cytoplasm cavi tion and organell decrease at 60 min and cytoarchitecture destroy and endoplasmic reticulum expand at 180 min. Acupuncture can two-sided regulate Ca2+ concentration and distribution of organell, de ;reased Ca2+ verloading, the alleviating the injury of neurons.The abnormal expressioi of SOD mRNA in rat brain tissue(cortex, hippocampus, striatum) was obvious at 3 hour after intracerebral hemorrhage(ICH), rose rapic y at 6 hour, peaked at 24 hour, then descended gradually at 3 da . Acupuncture can down-regulating the experimental of SODmRNA andd alleviating the injury of neurons. Conclusion: (i) Brain hem torn, brain edema formation following intracerebral tv morrhage(ICI I) lead to ischemia and hypoxia, Ca2+ overloading of ,he brain cell. The Ca2+ distribution of ultrastructural in the brain neuroh changed in time and pace, cytoarchitecture destroy and the peaked at 6) min. Acupuncture can play a role in inhibiting fluxed from the extracellular fluid into the intracellular space and descendedCa2+ overloading of brain cell and protected the neuronal injury inducey intracerebral hemorrhage. (2) The expression of SODmRNA is up-regulated in rat brain tissue that it was obvious at 3 hour after intracerebral hemorrhage(ICH), rose rapidly at 6 hour, peaked at 24 hour. Acupuncture can down-regulated the experimental of SODmRNA and alleviating the injury of neurons after intracerebral hemorrhage.