| The auther studied the Septoria microspra Speg.and the occurence rules of gentian leaf blight and also investigated the effect of gentian leaf blight on the output and quality of gentian by taking the weight of root and the content of efficient component of gentian as the index and established the effective controlling measures on agricultral measures,soil treatment ,seedling treatment, germicides control and traditional Chinese medicines control and the studies on the effect of these measures on the output and quality of gentian.had been carried out either.First, this experiment proved that the gentian leaf blight was caused by Septoria microspra Speg.and Septoria gentianae Thum.In this paper, the relation of the occurence of gentian leaf blight and cultivating factors had been studied .The method of graded counting had been used to investigate the relations of gentian leaf blight and the following factors:the grade of seedling,the cultivated density ,the cultivated time,the preceding crop,sunshading,the variety of gentian.The results showed that gentian leaf blight was reliefed when the following measures were applied:the first or second grade seedling was cultivated;the cultivated density was higher;cultivation began at autumn;interplanted technology was applied;rotation; the preceding crop was corn.Gentian leaf blight occured in Gentiana manshurica Kitag was lighter than that of Gentiana scabra Bunge.The effect of gentian leaf blight on the output and quality of gentian was studied in this experiment and the output loss model of gentian was established by determining the output of gentian and the loss of the efficient components and the account formula of control index was established also.The study of the effect of gentian leaf blight on the output of gentian showed that the disease could cause the decrease of the output of gentian and the decrease was obviously when the disease was severely. The output loss assessment model of gentian leaf blight in Heilongjiang was established by detected analyzing of different curves,the model was as following :(L- the radio of output loss ; d- the disease index; the correlativecoefficient r=0.8392 ; standard deviation s=10.5 ; the maximal value of loss radio K=54.11%) the account formula of control index was as following:(dET-the control index; W- cost; P-the price of crude drug; V-the anticipant output; M-the time for controlling disease; R-the aggravating speed of disease; Q-the controlling effect)HPLC was applied to determine the content of gentiopicroside and swertiamarin and the method of quality evaluation of gentian was established.The studied showed that the content of gentiopicroside was decreased when the disease index was increased, the decrease was not obviously when the index of disease was under 70,. the content began to drop obviously when the index of disease was above 70.The studied showed that the loss ratio of gentiopicroside and the loss of gentiopicroside was increased with the aggravation of the disease, the loss ratio of gentiopicroside and the loss of gentiopicroside was not obviously when the disease index was under 55 and was increased fastly when the disease index was above 55.The loss would be small when the disease index was under 60 .The results showed that gentian leaf blight was reliefed and the output and quality of gentian was higher when the following measures were applied:the first grade seedling was cultivatedjthe cultivated density was higher(125plant/m2); cultivation began at autumn; rotation.Spraied five germicides at different concentration on seedling bed in order to prevent the occurence of the disease of gentian. The results showed that:Carbendaxol(l:500) had the best effect of keeping seedling, carbendaxol(l:600), carbendaxol(l:700), chlorothalonil (1:500) and chlorothalonil(l:600) were secondly, the amount of keeping seedling was 5000 plant/m2, the amount of keeping seedling conformed with the standard of production.In this experiment ,the seedling was soaked by different germicide...
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