Isolation And Diversity Study Of Culturable Actinobacteria From Chinese Marine Sponges

The class Actinobacteria is a very important group of bacteria that has considerable value as a prolific producer of antibiotics and other therapeutic compounds. As the filter-feeding invertebrate animal, sponge accumulate large amount of microbes during the filtering process. It is urgent to study the isolation of actinobacteria from marine sponges for the exploration of this resource.To better understand the actinobacteria diversity associated with marine sponges, we attempted to isolate and cultivate actinobacteria from the intertidal marine sponge Hymeniacidon perleve for further physiological studies and to discover bioactive natural products. We selected 13 kinds of different actinobacteria isolation media and the results showed that the nutrient-rich media, in the addition of suitable inhibitors, could yield more actinobacteria from marine sponge Hymeniacidon perleve. This could be related with the relative nutrient host environment. The pre-treatments of heat and phenol to the sample did not improve the actinobacteria yield from the sponge. A total of 244 actinobacteria associated with the marine sponge Hymeniacidon perleve collected from the Yellow Sea, China were isolated using 13 different media. The number of species and genera of actinobacteria recovered from the different media varied significantly, underlining the importance of optimizing the isolation conditions. The phylogenetic diversity of the actinobacteria isolates was assessed using 16S rDNA amplification-restriction fragment length polymorphism (RFLP) analysis of 106...