Lush Film Sponge Microbial Diversity Research

The diversity of the bacterial community with marine sponge Hymeniacidonperleve was studied. In the first part of this work, combined with molecularanalysis morphological and physiological characteristics of three dominantisolates demonstrate that all of the 3 strains belong to γ-Proteobacteria. Two ofthem are Pseudoalteromonas.In the second part of this study, the variation of the microbial populationduring the process of sponge primmorphs cultivation was also studied, and thephylogenetic affiliation of the two dominants were determined by 16S rDNAanalysis.The phylogenetic affiliation of sponge-associated microorganisms wasdetermined by 16S rDNA sequencing of cloned DNA fragments. The sequences ofthe clone in sponge tissue were mainly attributed to be α-Proteobacteria,γ-Proteobacteria,Actinobacteria and low G+C% gram positive bacteria. The 16SrDNA sequences comparison between the 3 dominant culturable isolates and theclone in sponge tissue show that Pseudoalteromonas constantly exist.The same method was used to identify the microbial diversity in sponge cellculture. The sequences of the clones isolated from primmorph cultures weremainly attributed to be α-Proteobacteria,γ-Proteobacteria,Actinobacteriaand Plactomycete. Some sequences identified in the primmorphs are distantlyrelated to Pseudoalteromonas. All of the data show a higher probability ofPseudoalteromonas being the symbionts in sponge Hymeniacidon perleve.