Isolation And Bioactive Screening Of Secondary Metabolites From Sponge-associated Fungi Penicillium Chrysogenum And Cryptococcus Sp

Marine sponges, which are a group of multicellular marine organism, hold large amounts of microorganisms because of its unique ingestive and digestive system. Marine-derived microbes have proven to be a rich source of metabolites exhibiting a high rate of antibacterial, anticancer or even antivirus HIV activity; Therefore, to search for novel bioactive second products, we investigated the chemical constitutes and bioactivitives of the marine-derived fungi HLS-111(Penicillium chrysogenum) and HLS-105(Cryptococcus sp.) collected from LingShuiXian saemaul port waters in Hainan island.Forteen compounds were isolated from the low polarity extracts by repeated column chromatography on silica gel, Sephadex LH-20followed by High Performanc-e Liguid Chromatography. On the basis of spectroscopic methods (EI, ESI,’H-NMR,13C-NMR, DEPT),12compounds were elucidated as Secalonic F (1), Secalonic D (2), Ergosterol (3), β-sitosterol (4), Cyclicdiketopiperazine-L-phenylalanyl-L-tryptophan(5),1,3,8-Trihydroxy-6-methyl-9,10-anthraquinone (6), Stearic acid (7),(4E,8E)-N-D-2’-Hydroxy-palmitoyl-l-O-β-D-glyco-pyranosyl-9-methyl-4,8-sphingadienine(8),4-Hydroxybenzoic acid (9),24-Methylenecycloartanol trans-ferulate (10), Androst-7-en e-3,5,6-triol,17-[(1R,2E)-1,5,6-trimethyl-2-hepten-1-yl]-(3β,5α,6β,17β)-(11),(22E,24R)-5,8-Epidioxyergosta-6,22-dien-3-ol (12). The structures of compounds13and14are still in affirming. Compounds1,6,8,9and11were isolated from this genus for the first time while Compounds2,4and5were obtained from Penicillium chrysogenum for the first time. Five componds were isolated from petroleum ether portion of HLS-105. By the same spectroscopic methods, they were elucidated as Ergosterol (3),β-sitosterol (4),3-O-β-D-Glucosyl-β-sitosterol(15),β-D-Glucopyrano-side,(3)-stigmast-5-en-3-yl,6-decanoate (9C1)(16). Compounds4,15and16have never been reported from this genus before. The structure of compound17is still in affirming.Furthermore, bioassay results indicated that Compounds1and2showed significant antitumor activities to HCT-8, BEL-7402, BGC823, A549, A2780. Compounds2and13showed somewhat antivirus HIV activity; At the same time, compound13displayed anti-inflammatory activity.