Classification And Systematics Of The Aquilegiinae Tamura

Subtribe Aquilegiinae belong to the tribe Isopyreae of subfamily Isopyroidea, which was seperated from the tribe Isopyreae by staminode between the the inner stamen and capels. Characters as basic chromosome number 7, petal nearly sessile, saccate or spurred at the base, and the innermost numbers of stamens transformed into sterile staminode, which consists of three genus:Aquilegia, Semiaquilegia and Urophysa. The present study examined its habits, morphology, pollen morphology, seed surface and molecular phylogeny based on multiple regions of DNA sequences. We made a taxonomic research combining all of the available data on the Aquilegiinae. The results have been summarized as follows:1. Pollen morphologyPollen morphology of 16 taxa within Aquilegiinae was studied under Scanning Electron Microcopy (SEM). Combining the previous research on the pollen morphology, the present study indicated that: the plants of Aquilegiinae shared the common characters in pollen morphology. Their 3-colpate pollen grains are spheroidal, subprolate and prolate, the pollen could be divided into three types in genus Aquilegia. The pollen morphology has a few variations in Aquilegiinae, but it is of some significance for the specific classification within Aquilegia and the Aquilegiinae2. Seed surfaceSeed surface of 13 taxa in Aquilegiinae and 5 taxa of allies was first studied under Scanning Electron Microcopy (SEM). The result shows that characters such as the shape of epidermis, and the type of ornamentation of outer cell are variable between genera within Aquilegiinae and Isopyreae. Urophysa and Semiaquilega with verrucose papillae in outer cell wall are different from Aquilegia and the related genera out of Aquilegiinae, the difference is significant of the specific classification and systematic analysis in Aquilegiinae and Isopyreae.3. Phylogenetic analysis molecular sequence...